ID   COBQ_METAR              Reviewed;         487 AA.
AC   Q0W1N4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Probable cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; OrderedLocusNames=UNCMA_05470;
GN   ORFNames=RCIX2662;
OS   Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanocellales; Methanocellaceae; Methanocella.
OX   NCBI_TaxID=351160;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 22066 / NBRC 105507 / MRE50;
RX   PubMed=16857943; DOI=10.1126/science.1127062;
RA   Erkel C., Kube M., Reinhardt R., Liesack W.;
RT   "Genome of rice cluster I archaea -- the key methane producers in the rice
RT   rhizosphere.";
RL   Science 313:370-372(2006).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR   EMBL; AM114193; CAJ37709.1; -; Genomic_DNA.
DR   RefSeq; WP_012034876.1; NC_009464.1.
DR   AlphaFoldDB; Q0W1N4; -.
DR   SMR; Q0W1N4; -.
DR   STRING; 351160.RCIX2662; -.
DR   EnsemblBacteria; CAJ37709; CAJ37709; RCIX2662.
DR   GeneID; 5145445; -.
DR   KEGG; rci:RCIX2662; -.
DR   PATRIC; fig|351160.9.peg.572; -.
DR   eggNOG; arCOG00105; Archaea.
DR   OMA; EIHHGVA; -.
DR   OrthoDB; 34382at2157; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000000663; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..487
FT                   /note="Probable cobyric acid synthase"
FT                   /id="PRO_1000002380"
FT   DOMAIN          249..435
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        328
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        427
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   487 AA;  51959 MW;  56CBC643D7622C82 CRC64;
     MARYLTVLGT QSHVGKSILV TALCRILKRR GYRVAPFKAQ NMSNNSWITA DGSEIGIAQA
     IQAFAAGAEP RAEMNPVLLK PKGNMTSQVV VLGKPLGDRV VGQYYESIES MMAIALNALY
     KLSEDYDVIV VEGAGGAAEI NLYDRDIANI PLARAIHSPV LLVGDIERGG VFASLYGTIA
     LLPPGDRELV KGLIINKFCG DPSLLGSGVG ELEKLTGVPV MGIVPYSGLR IPSEDSVSLG
     DKKPEGLLDV DIAVVRFPLI SNFTDFEALE RIARVRYVSL DDSLGRPDIV ILPGSKNTVS
     DLKMLKNSPL ASEICSLASD GVPVIGICGG YQMLGRQVVD SGIEGGMPET IDGLGLLPVT
     TVFDRYEKCT CQSTKTVTGA GPLLAGIRGS AVTGYEIHMG QTRTDSPAFG DDGCVSDSGT
     ILGTYLHGIF NNASFTDAVL KYACERKGLR YVRPEGVRDP YDELADIVES AIDLDAIIRL
     IESQDKV