ID   COBQ_METJA              Reviewed;         492 AA.
AC   Q57908;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   25-MAY-2022, entry version 119.
DE   RecName: Full=Probable cobyric acid synthase;
GN   Name=cobQ; OrderedLocusNames=MJ0484;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation (By
CC       similarity). {ECO:0000250}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB98475.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; L77117; AAB98475.1; ALT_INIT; Genomic_DNA.
DR   PIR; D64360; D64360.
DR   RefSeq; WP_064496916.1; NC_000909.1.
DR   AlphaFoldDB; Q57908; -.
DR   STRING; 243232.MJ_0484; -.
DR   EnsemblBacteria; AAB98475; AAB98475; MJ_0484.
DR   GeneID; 1451346; -.
DR   KEGG; mja:MJ_0484; -.
DR   eggNOG; arCOG00105; Archaea.
DR   HOGENOM; CLU_019250_2_2_2; -.
DR   InParanoid; Q57908; -.
DR   OMA; EIHHGVA; -.
DR   OrthoDB; 34382at2157; -.
DR   PhylomeDB; Q57908; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..492
FT                   /note="Probable cobyric acid synthase"
FT                   /id="PRO_0000141348"
FT   DOMAIN          252..444
FT                   /note="GATase cobBQ-type"
FT   ACT_SITE        330
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        436
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   492 AA;  55419 MW;  0B8E8803B12E5F44 CRC64;
     MAEFIMVVGT SSNSGKTTIT AGLCRILANK GYKVAPFKSQ NMSLNSRVAK EDGEIAIAQY
     TQSLACRVEP SVHFNPILLK PKGNFISQVI VHGRPYKDMN YNEYRKNKDF FLKKIKESLE
     ILDREYDYVI MEGAGSCCEI NLLKDDIANL RIAELVNAKA ILVADIDRGG VFASIYGTIK
     LLPENWRKLI KGIIINKFRG NVEVLKEGIE KIEELTGIPV LGIVPYDENL VLPEEDSQVL
     QSMRSFGNAK SGVEINVVRF SKISNFTDLD PLRYDAFIKF IDFDDDITGD ILIFPGTRSS
     TKEAYYLKQH NFDEKVLEFL KDGGIVIGIC GGYQVLGKEL IDKEKKESDV GDIEGLKIFD
     AKTYFGNDKV VKNSCGFLEI DNKTFNVKGY EIHEGFTYSK EKPLIKIERG FGNCGNGFDG
     SIKKFGDGLA IGTYFHGIFE NYEFRNYIIN LIRKRKGLDE IYGDSYKDSI EKSLNYFAEV
     VERSVNLKPL GI