ID   COBQ_MICAN              Reviewed;         491 AA.
AC   B0JHX2;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; OrderedLocusNames=MAE_57120;
OS   Microcystis aeruginosa (strain NIES-843 / IAM M-2473).
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Microcystaceae; Microcystis.
OX   NCBI_TaxID=449447;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIES-843 / IAM M-247;
RX   PubMed=18192279; DOI=10.1093/dnares/dsm026;
RA   Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M.,
RA   Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A.,
RA   Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M.,
RA   Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M.;
RT   "Complete genomic structure of the bloom-forming toxic cyanobacterium
RT   Microcystis aeruginosa NIES-843.";
RL   DNA Res. 14:247-256(2007).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR   EMBL; AP009552; BAG05534.1; -; Genomic_DNA.
DR   RefSeq; WP_012267957.1; NC_010296.1.
DR   AlphaFoldDB; B0JHX2; -.
DR   STRING; 449447.MAE_57120; -.
DR   PaxDb; B0JHX2; -.
DR   EnsemblBacteria; BAG05534; BAG05534; MAE_57120.
DR   KEGG; mar:MAE_57120; -.
DR   PATRIC; fig|449447.4.peg.5221; -.
DR   eggNOG; COG1492; Bacteria.
DR   HOGENOM; CLU_019250_2_2_3; -.
DR   OMA; EIHHGVA; -.
DR   OrthoDB; 744477at2; -.
DR   BioCyc; MAER449447:MAE_RS24890-MON; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000001510; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..491
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_1000074401"
FT   DOMAIN          250..439
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        331
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        431
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   491 AA;  53963 MW;  B4B3B46DBC2C99FF CRC64;
     MKAIMAVGTT SHAGKSFLTA ALCRLFNRKG WQVTPFKGQN MALNAYVTAG GGEMGHAQAV
     QAWAAGTIPR VEMNPILLKP QGNMTSQVII KGKAVGVTTA VDYYQNYFEQ GWQAIKESLA
     KLSAEFDLVV CEGAGSPAEI NLKHRDLTNM RVAKYLDAAT ILVVDIDRGG AFAHVVGTLE
     LLEPEERALI KGIVINKFRG QKSLLDSGIT WLEDYTKIPV LGVLPYSDIF LSAEDSLSLL
     DRPAQKPKAE LNIIVIRLPH IANFTDFDPL CGEATVNVRY LELQESLGDP DGVIIPGSKT
     TVADLIALNQ SGMANQLQAY HQRGGIIFGI CGGLQMLGRL ILDTDHREGP ESEAAGLNLL
     PLKTVITAEK ITRQRQVLSN YPQGGLPVMG YEIHQGISQW ESESGYQKMF EEDASLGIVN
     DSLSIWGCYL HGIFDNGSWR RTWLNHLRQR RGLLSLPTGI ANYCEQREIT LDNMANLLEE
     HLNLQPIFAH L