COBQ_NOCFA
ID COBQ_NOCFA Reviewed; 520 AA.
AC Q5YSA4;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; OrderedLocusNames=NFA_40880;
OS Nocardia farcinica (strain IFM 10152).
OC Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Nocardia.
OX NCBI_TaxID=247156;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IFM 10152;
RX PubMed=15466710; DOI=10.1073/pnas.0406410101;
RA Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K.,
RA Shiba T., Hattori M.;
RT "The complete genomic sequence of Nocardia farcinica IFM 10152.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004).
CC -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC and one molecule of ATP is hydrogenolyzed for each amidation.
CC {ECO:0000255|HAMAP-Rule:MF_00028}.
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00028}.
CC -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR EMBL; AP006618; BAD58937.1; -; Genomic_DNA.
DR RefSeq; WP_011210622.1; NC_006361.1.
DR AlphaFoldDB; Q5YSA4; -.
DR SMR; Q5YSA4; -.
DR STRING; 247156.NFA_40880; -.
DR EnsemblBacteria; BAD58937; BAD58937; NFA_40880.
DR GeneID; 61134730; -.
DR KEGG; nfa:NFA_40880; -.
DR eggNOG; COG1492; Bacteria.
DR HOGENOM; CLU_019250_2_2_11; -.
DR OMA; EIHHGVA; -.
DR BioCyc; NFAR247156:NFA_RS20320-MON; -.
DR UniPathway; UPA00148; -.
DR Proteomes; UP000006820; Chromosome.
DR GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR CDD; cd01750; GATase1_CobQ; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR HAMAP; MF_00028; CobQ; 1.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR InterPro; IPR033949; CobQ_GATase1.
DR InterPro; IPR004459; CobQ_synth.
DR InterPro; IPR011698; GATase_3.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR Pfam; PF01656; CbiA; 1.
DR Pfam; PF07685; GATase_3; 1.
DR SUPFAM; SSF52317; SSF52317; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00313; cobQ; 1.
DR PROSITE; PS51274; GATASE_COBBQ; 1.
PE 3: Inferred from homology;
KW Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT CHAIN 1..520
FT /note="Cobyric acid synthase"
FT /id="PRO_0000141313"
FT DOMAIN 257..451
FT /note="GATase cobBQ-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT ACT_SITE 338
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT ACT_SITE 443
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ SEQUENCE 520 AA; 54607 MW; 9A3AA08EC5C95F0B CRC64;
MKGALLVAGT TSDAGKSVVV AGLCRMLARR GVRVAPFKAQ NMSNNSVVTL DGGEIGRAQA
LQARACGLEP SVRFNPVLLK PGSDRRSQLV VRGQAVDTVG AADYFRHRTA LREVVAAELA
SLRAEFDVVL CEGAGSPAEI NLRATDLANM GLARAADLPV LVVGDIDRGG VLAHLFGTVA
VLEPDDQRLI AGFVVNKFRG DVDLLRPGLD RLAELTGRPT LGVLPFAEEL WLDAEDSLGT
VADAPVGRPR PPVGTEWLTV AAVRLPRISN STDVEALACE PGVAVRWVSE PSRLADADLV
VVPGSKSTVS DLAWLRRTGL GEALRARVTA GRPVLAICGG YQMLGRRIVD EVESGAGEVA
GLGVFDLETE FAAPKVLRRV TGMGAGNPVR GYEIHHGRVR RSGDAPWLGL VDAPEAAVDG
GEDAVPLTPE GSVTGGAWGT HVHGLLESDG FRRAWLREVA ARAGRHGFQV APDTSVAAVR
AGQLDLLADL VEKHLDQDAL ERILTDGAPT GLPVLTVGQA
