ID   COBQ_NOSP7              Reviewed;         494 AA.
AC   B2J764;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; OrderedLocusNames=Npun_F0495;
OS   Nostoc punctiforme (strain ATCC 29133 / PCC 73102).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=63737;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29133 / PCC 73102;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C., Elhai J.,
RA   Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.;
RT   "Complete sequence of chromosome of Nostoc punctiforme ATCC 29133.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR   EMBL; CP001037; ACC79272.1; -; Genomic_DNA.
DR   RefSeq; WP_012407297.1; NC_010628.1.
DR   AlphaFoldDB; B2J764; -.
DR   SMR; B2J764; -.
DR   STRING; 63737.Npun_F0495; -.
DR   EnsemblBacteria; ACC79272; ACC79272; Npun_F0495.
DR   KEGG; npu:Npun_F0495; -.
DR   eggNOG; COG1492; Bacteria.
DR   HOGENOM; CLU_019250_2_2_3; -.
DR   OMA; EIHHGVA; -.
DR   OrthoDB; 744477at2; -.
DR   PhylomeDB; B2J764; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000001191; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..494
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_1000090237"
FT   DOMAIN          252..444
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        333
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        436
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   494 AA;  54343 MW;  E82E1B036F2A96CF CRC64;
     MKSIMVVGTT SHAGKSLLTT AICRILSRRG WRVAPFKGQN MALNAYVTAS GGEIGYAQAV
     QAWAAGVVPW VEMNPILLKP QGDMTSQVII KGRSVGKVSA SDYYEQYFEL GWRTIEESLQ
     HLGTEFDLLV CEGAGSPAEI NLKHRDLTNM RVAKYLNAPT MLVVDIDRGG AFAHVVGTLE
     LLEPDERALI KGVVINKFRG QRSLLDPGIK WLEERTGIPV IGVIPYLQEV FSTEDSLDLL
     ERQSSSSKAQ TDLNIAVIRL PRIANFTDFD PLESESTVSV KYLSPKQDLG HPDAVIIPGT
     KTTIADLLLL QKSGMAEAIQ HYAASGGTVL GICGGYQMLG QIIADPEGIE GQAGRFQGLN
     LLPIRTVITG QKIARQRQVS SNYPQQGLPV NGFEIHQGRS RIEQQGIDPQ SYHALFDDIN
     LGLVDSCQSV WGSYLHGLFD NGPWRRAWLN RLRQQRGLKS LPTGVANYRE QREQILDSLA
     TEVESHLDLT PFLS