ID   COBQ_PSEAE              Reviewed;         490 AA.
AC   Q9I467;
DT   04-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; OrderedLocusNames=PA1277;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR   EMBL; AE004091; AAG04666.1; -; Genomic_DNA.
DR   PIR; C83486; C83486.
DR   RefSeq; NP_249968.1; NC_002516.2.
DR   RefSeq; WP_003112353.1; NZ_QZGE01000005.1.
DR   AlphaFoldDB; Q9I467; -.
DR   SMR; Q9I467; -.
DR   STRING; 287.DR97_658; -.
DR   PaxDb; Q9I467; -.
DR   EnsemblBacteria; AAG04666; AAG04666; PA1277.
DR   GeneID; 881421; -.
DR   KEGG; pae:PA1277; -.
DR   PATRIC; fig|208964.12.peg.1327; -.
DR   PseudoCAP; PA1277; -.
DR   HOGENOM; CLU_019250_2_2_6; -.
DR   InParanoid; Q9I467; -.
DR   OMA; EIHHGVA; -.
DR   PhylomeDB; Q9I467; -.
DR   BioCyc; PAER208964:G1FZ6-1302-MON; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..490
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_0000141320"
FT   DOMAIN          252..439
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        333
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        431
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   490 AA;  52372 MW;  473D9B618A2B2757 CRC64;
     MSDRGRTLMV QGTTSDAGKS TLVTALCRWL ARRGVAVVPF KPQNMALNSA VTADGGEIGR
     AQAVQAQACR LAPHTDMNPV LLKPNTDIGA QVIIHGRAVT SMDAAAYHDY KRVAMEAVLA
     SHGRLAAAYR VVMVEGAGSP AEINLRANDI ANMGFAEAVD CPVILVADID RGGVFAHLVG
     TLELLSDSER ERVKGFVINR FRGDIALLQP GLDWLEARTG KPVLGVLPYV SDLHLEAEDA
     IDTRQAAKVG PRLKVVVPVL PRISNHTDFD PLRLHPQVEL SFVGPGQALP SADLIVLPGS
     KSVRADLAAL RERGWDEAIL RHLRYGGRLL GICGGLQMLG ERLHDPLGLE GAAGSSAGLG
     LLALETTLEA DKQLRNVQGR LSLEDAPLSG YEIHAGVTRG EALARPAVVL DDGRADGARS
     VDGNVMGTYL HGLFESTAAC SALLRWAGLR EVQAVDYQAL RERDIERLAD LVERHLDTGR
     LLALCGEPHA