ACOT8_MOUSE
ID ACOT8_MOUSE Reviewed; 320 AA.
AC P58137; Q8VHM4;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Acyl-coenzyme A thioesterase 8;
DE Short=Acyl-CoA thioesterase 8;
DE EC=3.1.2.1 {ECO:0000269|PubMed:11673457};
DE EC=3.1.2.11 {ECO:0000269|PubMed:11673457};
DE EC=3.1.2.2 {ECO:0000269|PubMed:11673457};
DE EC=3.1.2.3 {ECO:0000269|PubMed:16141203};
DE EC=3.1.2.5 {ECO:0000269|PubMed:11673457};
DE AltName: Full=Choloyl-coenzyme A thioesterase;
DE EC=3.1.2.27 {ECO:0000269|PubMed:11673457};
DE AltName: Full=Peroxisomal acyl-CoA thioesterase 2 {ECO:0000303|PubMed:11673457};
DE Short=PTE-2 {ECO:0000303|PubMed:11673457};
DE AltName: Full=Peroxisomal acyl-coenzyme A thioester hydrolase 1;
DE Short=PTE-1;
DE AltName: Full=Peroxisomal long-chain acyl-CoA thioesterase 1;
GN Name=Acot8; Synonyms=Pte1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, ACTIVITY
RP REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=129/Sv;
RX PubMed=11673457; DOI=10.1074/jbc.m106458200;
RA Hunt M.C., Solaas K., Kase B.F., Alexson S.E.H.;
RT "Characterization of an acyl-CoA thioesterase that functions as a major
RT regulator of peroxisomal lipid metabolism.";
RL J. Biol. Chem. 277:1128-1138(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=16141203; DOI=10.1074/jbc.m508479200;
RA Westin M.A., Hunt M.C., Alexson S.E.;
RT "The identification of a succinyl-CoA thioesterase suggests a novel pathway
RT for succinate production in peroxisomes.";
RL J. Biol. Chem. 280:38125-38132(2005).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Kidney, Liver, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Catalyzes the hydrolysis of acyl-CoAs into free fatty acids
CC and coenzyme A (CoASH), regulating their respective intracellular
CC levels (PubMed:11673457). Displays no strong substrate specificity with
CC respect to the carboxylic acid moiety of Acyl-CoAs (PubMed:11673457).
CC Hydrolyzes medium length (C2 to C20) straight-chain, saturated and
CC unsaturated acyl-CoAS but is inactive towards substrates with longer
CC aliphatic chains (PubMed:11673457). Moreover, it catalyzes the
CC hydrolysis of CoA esters of bile acids, such as choloyl-CoA and
CC chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-
CC acyltransferase (BAAT) (PubMed:11673457). Is also able to hydrolyze CoA
CC esters of dicarboxylic acids (PubMed:16141203). It is involved in the
CC metabolic regulation of peroxisome proliferation (By similarity).
CC {ECO:0000250|UniProtKB:O14734, ECO:0000269|PubMed:11673457,
CC ECO:0000269|PubMed:16141203}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=choloyl-CoA + H2O = cholate + CoA + H(+);
CC Xref=Rhea:RHEA:14541, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29747, ChEBI:CHEBI:57287, ChEBI:CHEBI:57373; EC=3.1.2.27;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14542;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chenodeoxycholoyl-CoA + H2O = chenodeoxycholate + CoA + H(+);
CC Xref=Rhea:RHEA:31511, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:36234, ChEBI:CHEBI:57287, ChEBI:CHEBI:62989; EC=3.1.2.27;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31512;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O = acetate + CoA + H(+); Xref=Rhea:RHEA:20289,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=3.1.2.1;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20290;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + malonyl-CoA = CoA + H(+) + malonate;
CC Xref=Rhea:RHEA:40219, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15792, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384;
CC Evidence={ECO:0000269|PubMed:11673457, ECO:0000269|PubMed:16141203};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40220;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetoacetyl-CoA + H2O = acetoacetate + CoA + H(+);
CC Xref=Rhea:RHEA:15673, ChEBI:CHEBI:13705, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57286, ChEBI:CHEBI:57287; EC=3.1.2.11;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15674;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + propanoyl-CoA = CoA + H(+) + propanoate;
CC Xref=Rhea:RHEA:40103, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17272, ChEBI:CHEBI:57287, ChEBI:CHEBI:57392;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40104;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=butanoyl-CoA + H2O = butanoate + CoA + H(+);
CC Xref=Rhea:RHEA:40111, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17968, ChEBI:CHEBI:57287, ChEBI:CHEBI:57371;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40112;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + succinyl-CoA = CoA + H(+) + succinate;
CC Xref=Rhea:RHEA:11516, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30031, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292; EC=3.1.2.3;
CC Evidence={ECO:0000269|PubMed:16141203};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11517;
CC Evidence={ECO:0000305|PubMed:16141203};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glutaryl-CoA + H2O = CoA + glutarate + H(+);
CC Xref=Rhea:RHEA:40575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30921, ChEBI:CHEBI:57287, ChEBI:CHEBI:57378;
CC Evidence={ECO:0000269|PubMed:16141203};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40576;
CC Evidence={ECO:0000305|PubMed:16141203};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + hexanoyl-CoA = CoA + H(+) + hexanoate;
CC Xref=Rhea:RHEA:40115, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17120, ChEBI:CHEBI:57287, ChEBI:CHEBI:62620;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40116;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + hexanedioyl-CoA = CoA + H(+) + hexanedioate;
CC Xref=Rhea:RHEA:40583, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17128, ChEBI:CHEBI:57287, ChEBI:CHEBI:76327;
CC Evidence={ECO:0000269|PubMed:16141203};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40584;
CC Evidence={ECO:0000305|PubMed:16141203};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + octanoyl-CoA = CoA + H(+) + octanoate;
CC Xref=Rhea:RHEA:30143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:25646, ChEBI:CHEBI:57287, ChEBI:CHEBI:57386;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30144;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + octanedioyl-CoA = CoA + H(+) + octanedioate;
CC Xref=Rhea:RHEA:40587, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:76282, ChEBI:CHEBI:76317;
CC Evidence={ECO:0000269|PubMed:16141203};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40588;
CC Evidence={ECO:0000305|PubMed:16141203};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=decanoyl-CoA + H2O = CoA + decanoate + H(+);
CC Xref=Rhea:RHEA:40059, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:27689, ChEBI:CHEBI:57287, ChEBI:CHEBI:61430;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40060;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=decanedioyl-CoA + H2O = CoA + decanedioate + H(+);
CC Xref=Rhea:RHEA:40591, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:76283, ChEBI:CHEBI:76316;
CC Evidence={ECO:0000269|PubMed:16141203};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40592;
CC Evidence={ECO:0000305|PubMed:16141203};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dodecanoyl-CoA + H2O = CoA + dodecanoate + H(+);
CC Xref=Rhea:RHEA:30135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:18262, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30136;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dodecanedioyl-CoA + H2O = CoA + dodecanedioate + H(+);
CC Xref=Rhea:RHEA:40595, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:76273, ChEBI:CHEBI:76315;
CC Evidence={ECO:0000269|PubMed:16141203};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40596;
CC Evidence={ECO:0000305|PubMed:16141203};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + tetradecanoyl-CoA = CoA + H(+) + tetradecanoate;
CC Xref=Rhea:RHEA:40119, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30807, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40120;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-tetradecenoyl-CoA + H2O = (9Z)-tetradecenoate + CoA +
CC H(+); Xref=Rhea:RHEA:40135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:32370, ChEBI:CHEBI:57287, ChEBI:CHEBI:65060;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40136;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate;
CC Xref=Rhea:RHEA:16645, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379; EC=3.1.2.2;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16646;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-hexadecenoyl-CoA + H2O = (9Z)-hexadecenoate + CoA + H(+);
CC Xref=Rhea:RHEA:40131, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:32372, ChEBI:CHEBI:57287, ChEBI:CHEBI:61540;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40132;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + octadecanoyl-CoA = CoA + H(+) + octadecanoate;
CC Xref=Rhea:RHEA:30139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:25629, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30140;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+);
CC Xref=Rhea:RHEA:40139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30823, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40140;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + H2O = (9Z,12Z)-octadecadienoate
CC + CoA + H(+); Xref=Rhea:RHEA:40143, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30245, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57383; Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40144;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=eicosanoyl-CoA + H2O = CoA + eicosanoate + H(+);
CC Xref=Rhea:RHEA:40147, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:32360, ChEBI:CHEBI:57287, ChEBI:CHEBI:57380;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40148;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-
CC eicosatetraenoate + CoA + H(+); Xref=Rhea:RHEA:40151,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32395,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57368;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40152;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4,8-dimethylnonanoyl-CoA + H2O = 4,8-dimethylnonanoate + CoA +
CC H(+); Xref=Rhea:RHEA:40223, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77061, ChEBI:CHEBI:77063;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40224;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2,6-dimethylheptanoyl-CoA + H2O = 2,6-dimethylheptanoate + CoA
CC + H(+); Xref=Rhea:RHEA:59952, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:84847, ChEBI:CHEBI:143533;
CC Evidence={ECO:0000250|UniProtKB:Q8VHK0};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59953;
CC Evidence={ECO:0000250|UniProtKB:Q8VHK0};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3S)-hydroxy-3-methylglutaryl-CoA + H2O = 3-hydroxy-3-
CC methylglutarate + CoA + H(+); Xref=Rhea:RHEA:16305,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17325,
CC ChEBI:CHEBI:43074, ChEBI:CHEBI:57287; EC=3.1.2.5;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16306;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA +
CC H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA
CC + H(+); Xref=Rhea:RHEA:59936, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:63001, ChEBI:CHEBI:85674;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59937;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-methyloctadecanoyl-CoA + H2O = 2-methyloctadecanoate + CoA +
CC H(+); Xref=Rhea:RHEA:59940, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:143530, ChEBI:CHEBI:143531;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59941;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + prostaglandin F2alpha-CoA = CoA + H(+) + prostaglandin
CC F2alpha; Xref=Rhea:RHEA:59948, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57404, ChEBI:CHEBI:143532;
CC Evidence={ECO:0000269|PubMed:11673457};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59949;
CC Evidence={ECO:0000305|PubMed:11673457};
CC -!- ACTIVITY REGULATION: Inhibited by CoASH (IC(50)=10-15 uM). Also
CC inhibited by cysteine-reactive agents. {ECO:0000269|PubMed:11673457}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=29.4 uM for acetyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=8.0 uM for propionyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=22.6 uM for butyryl-CoA {ECO:0000269|PubMed:11673457};
CC KM=23.4 uM for hexanoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=6.9 uM for octanoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=2.9 uM for decanoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=2.8 uM for lauroyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=2.5 uM for myristoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=3.5 uM for myristoleoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=1.7 uM for palmitoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=1.4 uM for palmitoleoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=2.7 uM for stearoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=1.6 uM for oleoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=2.3 uM for linoleoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=4.2 uM for arachidoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=6.7 uM for arachidonoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=6.3 uM for 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-
CC oyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=14.6 uM for choloyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=8.8 uM for chenodeoxycholoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=5.5 uM for 4,8-dimethylnonanoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=0.92 uM for prostaglandin F2alpha-CoA
CC {ECO:0000269|PubMed:11673457};
CC KM=1.9 uM for 2-methyloctadecanoyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=22.9 uM for (3S)-hydroxy-3-methylglutaryl-CoA
CC {ECO:0000269|PubMed:11673457};
CC KM=12.9 uM for malonyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=22.9 uM for acetoacetyl-CoA {ECO:0000269|PubMed:11673457};
CC KM=34.7 uM for succinyl-CoA {ECO:0000269|PubMed:16141203};
CC KM=15.9 uM for glutaryl-CoA {ECO:0000269|PubMed:16141203};
CC KM=21.8 uM for hexanedioyl-CoA {ECO:0000269|PubMed:16141203};
CC KM=19.8 uM for octanoyl-CoA {ECO:0000269|PubMed:16141203};
CC KM=10.5 uM for decanedioyl-CoA {ECO:0000269|PubMed:16141203};
CC KM=14.1 uM for dodecanedioyl-CoA {ECO:0000269|PubMed:16141203};
CC Vmax=4.6 umol/min/mg enzyme with acetyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=3.45 umol/min/mg enzyme with propionyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=2.6 umol/min/mg enzyme with butyryl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=5.6 umol/min/mg enzyme with hexanoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=3.9 umol/min/mg enzyme with octanoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=3.8 umol/min/mg enzyme with decanoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=3.0 umol/min/mg enzyme with lauroyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=3.6 umol/min/mg enzyme with myristoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=3.2 umol/min/mg enzyme with myristoleoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=1.7 umol/min/mg enzyme with palmitoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=2.7 umol/min/mg enzyme with palmitoleoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=3.2 umol/min/mg enzyme with stearoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=3.1 umol/min/mg enzyme with oleoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=2.3 umol/min/mg enzyme with linoleoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=2.2 umol/min/mg enzyme with arachidoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=1.6 umol/min/mg enzyme with arachidonoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=8.1 umol/min/mg enzyme with 3alpha,7alpha,12alpha-trihydroxy-
CC 5beta-cholestan-26-oyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=17.9 umol/min/mg enzyme with choloyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=17.1 umol/min/mg enzyme with chenodeoxycholoyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=10.69 umol/min/mg enzyme with 4,8-dimethylnonanoyl-CoA as
CC substrate {ECO:0000269|PubMed:11673457};
CC Vmax=2.25 umol/min/mg enzyme with prostaglandin F2alpha-CoA as
CC substrate {ECO:0000269|PubMed:11673457};
CC Vmax=1.65 umol/min/mg enzyme with 2-methyloctadecanoyl-CoA as
CC substrate {ECO:0000269|PubMed:11673457};
CC Vmax=2.85 umol/min/mg enzyme with (3S)-hydroxy-3-methylglutaryl-CoA
CC as substrate {ECO:0000269|PubMed:11673457};
CC Vmax=1.19 umol/min/mg enzyme with malonyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=6.0 umol/min/mg enzyme with acetoacetyl-CoA as substrate
CC {ECO:0000269|PubMed:11673457};
CC Vmax=0.82 umol/min/mg enzyme with succinyl-CoA as substrate
CC {ECO:0000269|PubMed:16141203};
CC Vmax=2.35 umol/min/mg enzyme with glutaryl-CoA as substrate
CC {ECO:0000269|PubMed:16141203};
CC Vmax=3.4 umol/min/mg enzyme with hexanedioyl-CoA as substrate
CC {ECO:0000269|PubMed:16141203};
CC Vmax=4.13 umol/min/mg enzyme with octanoyl-CoA as substrate
CC {ECO:0000269|PubMed:16141203};
CC Vmax=3.82 umol/min/mg enzyme with decanedioyl-CoA as substrate
CC {ECO:0000269|PubMed:16141203};
CC Vmax=5.03 umol/min/mg enzyme with dodecanedioyl-CoA as substrate
CC {ECO:0000269|PubMed:16141203};
CC Note=In summary, KM for medium- to long-chain acyl CoAs is in the
CC order 1.4-6.7 uM, and with short-chain acyl CoAs range from 8 to 30
CC uM. KM for bile acid-CoA esters is in the range 6-15 uM.
CC {ECO:0000269|PubMed:11673457};
CC -!- PATHWAY: Lipid metabolism; fatty acid metabolism.
CC {ECO:0000305|PubMed:11673457}.
CC -!- SUBUNIT: homodimer. {ECO:0000269|PubMed:11673457}.
CC -!- SUBCELLULAR LOCATION: Peroxisome matrix {ECO:0000269|PubMed:11673457}.
CC Note=Predominantly localized in the peroxisome but a localization to
CC the cytosol cannot be excluded. {ECO:0000250|UniProtKB:O14734}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:11673457}.
CC -!- INDUCTION: Induced in the liver, by peroxisome proliferator or fasting
CC via the peroxisome proliferator-activated receptors (PPARs). Diurnal
CC regulation of its expression. {ECO:0000269|PubMed:11673457}.
CC -!- MISCELLANEOUS: Constitutes about 1% of total peroxisomal protein.
CC -!- SIMILARITY: Belongs to the C/M/P thioester hydrolase family.
CC {ECO:0000305}.
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DR EMBL; AF441166; AAL35333.1; -; mRNA.
DR EMBL; BC005792; AAH05792.1; -; mRNA.
DR CCDS; CCDS17057.1; -.
DR RefSeq; NP_573503.2; NM_133240.2.
DR AlphaFoldDB; P58137; -.
DR SMR; P58137; -.
DR BioGRID; 228440; 14.
DR STRING; 10090.ENSMUSP00000099383; -.
DR SwissLipids; SLP:000000534; -.
DR iPTMnet; P58137; -.
DR PhosphoSitePlus; P58137; -.
DR EPD; P58137; -.
DR jPOST; P58137; -.
DR PaxDb; P58137; -.
DR PeptideAtlas; P58137; -.
DR PRIDE; P58137; -.
DR ProteomicsDB; 285654; -.
DR Antibodypedia; 4138; 250 antibodies from 28 providers.
DR DNASU; 170789; -.
DR Ensembl; ENSMUST00000103094; ENSMUSP00000099383; ENSMUSG00000017307.
DR GeneID; 170789; -.
DR KEGG; mmu:170789; -.
DR UCSC; uc008nwf.2; mouse.
DR CTD; 10005; -.
DR MGI; MGI:2158201; Acot8.
DR VEuPathDB; HostDB:ENSMUSG00000017307; -.
DR eggNOG; KOG3016; Eukaryota.
DR GeneTree; ENSGT00390000004207; -.
DR HOGENOM; CLU_032690_1_0_1; -.
DR InParanoid; P58137; -.
DR OMA; SQVWFRT; -.
DR OrthoDB; 826588at2759; -.
DR PhylomeDB; P58137; -.
DR TreeFam; TF315124; -.
DR BRENDA; 3.1.2.2; 3474.
DR BRENDA; 3.1.2.20; 3474.
DR BRENDA; 3.1.2.27; 3474.
DR Reactome; R-MMU-193368; Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
DR Reactome; R-MMU-2046106; alpha-linolenic acid (ALA) metabolism.
DR Reactome; R-MMU-389887; Beta-oxidation of pristanoyl-CoA.
DR Reactome; R-MMU-390247; Beta-oxidation of very long chain fatty acids.
DR Reactome; R-MMU-9033241; Peroxisomal protein import.
DR UniPathway; UPA00199; -.
DR BioGRID-ORCS; 170789; 2 hits in 75 CRISPR screens.
DR ChiTaRS; Acot8; mouse.
DR PRO; PR:P58137; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; P58137; protein.
DR Bgee; ENSMUSG00000017307; Expressed in spermatid and 250 other tissues.
DR ExpressionAtlas; P58137; baseline and differential.
DR Genevisible; P58137; MM.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005782; C:peroxisomal matrix; ISO:MGI.
DR GO; GO:0005777; C:peroxisome; IDA:HGNC-UCL.
DR GO; GO:0047603; F:acetoacetyl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0003986; F:acetyl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0047617; F:acyl-CoA hydrolase activity; IDA:MGI.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0033882; F:choloyl-CoA hydrolase activity; ISO:MGI.
DR GO; GO:0044466; F:glutaryl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0047994; F:hydroxymethylglutaryl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0052815; F:medium-chain acyl-CoA hydrolase activity; ISS:UniProtKB.
DR GO; GO:0102991; F:myristoyl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0016290; F:palmitoyl-CoA hydrolase activity; ISS:UniProtKB.
DR GO; GO:0004778; F:succinyl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0006637; P:acyl-CoA metabolic process; IDA:MGI.
DR GO; GO:0043649; P:dicarboxylic acid catabolic process; ISO:MGI.
DR GO; GO:0009062; P:fatty acid catabolic process; IBA:GO_Central.
DR GO; GO:0045225; P:negative regulation of CD4 production; ISS:UniProtKB.
DR GO; GO:0016559; P:peroxisome fission; ISO:MGI.
DR Gene3D; 2.40.160.210; -; 1.
DR InterPro; IPR042171; Acyl-CoA_hotdog.
DR InterPro; IPR003703; Acyl_CoA_thio.
DR InterPro; IPR029069; HotDog_dom_sf.
DR PANTHER; PTHR11066; PTHR11066; 1.
DR SUPFAM; SSF54637; SSF54637; 2.
DR TIGRFAMs; TIGR00189; tesB; 1.
PE 1: Evidence at protein level;
KW Fatty acid metabolism; Hydrolase; Lipid metabolism; Peroxisome;
KW Peroxisome biogenesis; Reference proteome; Serine esterase.
FT CHAIN 1..320
FT /note="Acyl-coenzyme A thioesterase 8"
FT /id="PRO_0000202153"
FT MOTIF 318..320
FT /note="Microbody targeting signal"
FT /evidence="ECO:0000255"
FT ACT_SITE 233
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 255
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 305
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT CONFLICT 284
FT /note="S -> F (in Ref. 1; AAL35333)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 320 AA; 35827 MW; 94F6AFCFEEE2FA23 CRC64;
MSAPEGLGDA HGDADRGDLS GDLRSVLVTS VLNLEPLDED LYRGRHYWVP TSQRLFGGQI
MGQALVAAAK SVSEDVHVHS LHCYFVRAGD PKVPVLYHVE RIRTGASFSV RAVKAVQHGK
AIFICQASFQ QMQPSPLQHQ FSMPSVPPPE DLLDHEALID QYLRDPNLHK KYRVGLNRVA
AQEVPIEIKV VNPPTLTQLQ ALEPKQMFWV RARGYIGEGD IKMHCCVAAY ISDYAFLGTA
LLPHQSKYKV NFMASLDHSM WFHAPFRADH WMLYECESPW AGGSRGLVHG RLWRRDGVLA
VTCAQEGVIR LKPQVSESKL
