ACOT8_RAT
ID ACOT8_RAT Reviewed; 320 AA.
AC Q8VHK0;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Acyl-coenzyme A thioesterase 8;
DE Short=Acyl-CoA thioesterase 8;
DE EC=3.1.2.1 {ECO:0000269|PubMed:11785945};
DE EC=3.1.2.11 {ECO:0000250|UniProtKB:P58137};
DE EC=3.1.2.2 {ECO:0000250|UniProtKB:P58137};
DE EC=3.1.2.3 {ECO:0000250|UniProtKB:P58137};
DE EC=3.1.2.5 {ECO:0000250|UniProtKB:P58137};
DE AltName: Full=Choloyl-coenzyme A thioesterase;
DE EC=3.1.2.27 {ECO:0000269|PubMed:11785945};
DE AltName: Full=Peroxisomal acyl-coenzyme A thioester hydrolase 1 {ECO:0000303|PubMed:11785945};
DE Short=PTE-1 {ECO:0000303|PubMed:11785945};
DE AltName: Full=Peroxisomal long-chain acyl-CoA thioesterase 1;
GN Name=Acot8;
GN Synonyms=Pte1 {ECO:0000303|PubMed:10092594, ECO:0000303|PubMed:11785945};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR
RP LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11785945; DOI=10.1006/bbrc.2001.6245;
RA Ofman R., el Mrabet L., Dacremont G., Spijer D., Wanders R.J.;
RT "Demonstration of dimethylnonanoyl-CoA thioesterase activity in rat liver
RT peroxisomes followed by purification and molecular cloning of the
RT thioesterase involved.";
RL Biochem. Biophys. Res. Commun. 290:629-634(2002).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=10092594; DOI=10.1074/jbc.274.14.9216;
RA Jones J.M., Nau K., Geraghty M.T., Erdmann R., Gould S.J.;
RT "Identification of peroxisomal acyl-CoA thioesterases in yeast and
RT humans.";
RL J. Biol. Chem. 274:9216-9223(1999).
CC -!- FUNCTION: Catalyzes the hydrolysis of acyl-CoAs into free fatty acids
CC and coenzyme A (CoASH), regulating their respective intracellular
CC levels (PubMed:11785945). Displays no strong substrate specificity with
CC respect to the carboxylic acid moiety of Acyl-CoAs (By similarity).
CC Hydrolyzes medium length (C2 to C20) straight-chain, saturated and
CC unsaturated acyl-CoAS but is inactive towards substrates with longer
CC aliphatic chains (PubMed:11785945). Moreover, it catalyzes the
CC hydrolysis of CoA esters of bile acids, such as choloyl-CoA and
CC chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-
CC acyltransferase (BAAT) (By similarity). Is also able to hydrolyze CoA
CC esters of dicarboxylic acids (By similarity). It is involved in the
CC metabolic regulation of peroxisome proliferation (By similarity).
CC {ECO:0000250|UniProtKB:O14734, ECO:0000250|UniProtKB:P58137,
CC ECO:0000269|PubMed:11785945}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=choloyl-CoA + H2O = cholate + CoA + H(+);
CC Xref=Rhea:RHEA:14541, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29747, ChEBI:CHEBI:57287, ChEBI:CHEBI:57373; EC=3.1.2.27;
CC Evidence={ECO:0000269|PubMed:11785945};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14542;
CC Evidence={ECO:0000305|PubMed:11785945};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chenodeoxycholoyl-CoA + H2O = chenodeoxycholate + CoA + H(+);
CC Xref=Rhea:RHEA:31511, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:36234, ChEBI:CHEBI:57287, ChEBI:CHEBI:62989; EC=3.1.2.27;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31512;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O = acetate + CoA + H(+); Xref=Rhea:RHEA:20289,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=3.1.2.1;
CC Evidence={ECO:0000269|PubMed:11785945};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20290;
CC Evidence={ECO:0000305|PubMed:11785945};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=butanoyl-CoA + H2O = butanoate + CoA + H(+);
CC Xref=Rhea:RHEA:40111, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17968, ChEBI:CHEBI:57287, ChEBI:CHEBI:57371;
CC Evidence={ECO:0000269|PubMed:11785945};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40112;
CC Evidence={ECO:0000305|PubMed:11785945};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + hexanoyl-CoA = CoA + H(+) + hexanoate;
CC Xref=Rhea:RHEA:40115, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17120, ChEBI:CHEBI:57287, ChEBI:CHEBI:62620;
CC Evidence={ECO:0000269|PubMed:11785945};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40116;
CC Evidence={ECO:0000305|PubMed:11785945};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + octanoyl-CoA = CoA + H(+) + octanoate;
CC Xref=Rhea:RHEA:30143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:25646, ChEBI:CHEBI:57287, ChEBI:CHEBI:57386;
CC Evidence={ECO:0000269|PubMed:11785945};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30144;
CC Evidence={ECO:0000305|PubMed:11785945};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=decanoyl-CoA + H2O = CoA + decanoate + H(+);
CC Xref=Rhea:RHEA:40059, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:27689, ChEBI:CHEBI:57287, ChEBI:CHEBI:61430;
CC Evidence={ECO:0000269|PubMed:11785945};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40060;
CC Evidence={ECO:0000305|PubMed:11785945};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dodecanoyl-CoA + H2O = CoA + dodecanoate + H(+);
CC Xref=Rhea:RHEA:30135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:18262, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375;
CC Evidence={ECO:0000269|PubMed:11785945};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30136;
CC Evidence={ECO:0000305|PubMed:11785945};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + tetradecanoyl-CoA = CoA + H(+) + tetradecanoate;
CC Xref=Rhea:RHEA:40119, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30807, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385;
CC Evidence={ECO:0000269|PubMed:11785945};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40120;
CC Evidence={ECO:0000305|PubMed:11785945};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4,8-dimethylnonanoyl-CoA + H2O = 4,8-dimethylnonanoate + CoA +
CC H(+); Xref=Rhea:RHEA:40223, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77061, ChEBI:CHEBI:77063;
CC Evidence={ECO:0000269|PubMed:11785945};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40224;
CC Evidence={ECO:0000305|PubMed:11785945};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2,6-dimethylheptanoyl-CoA + H2O = 2,6-dimethylheptanoate + CoA
CC + H(+); Xref=Rhea:RHEA:59952, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:84847, ChEBI:CHEBI:143533;
CC Evidence={ECO:0000269|PubMed:11785945};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59953;
CC Evidence={ECO:0000305|PubMed:11785945};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + malonyl-CoA = CoA + H(+) + malonate;
CC Xref=Rhea:RHEA:40219, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15792, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40220;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetoacetyl-CoA + H2O = acetoacetate + CoA + H(+);
CC Xref=Rhea:RHEA:15673, ChEBI:CHEBI:13705, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57286, ChEBI:CHEBI:57287; EC=3.1.2.11;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15674;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + propanoyl-CoA = CoA + H(+) + propanoate;
CC Xref=Rhea:RHEA:40103, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17272, ChEBI:CHEBI:57287, ChEBI:CHEBI:57392;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40104;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + succinyl-CoA = CoA + H(+) + succinate;
CC Xref=Rhea:RHEA:11516, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30031, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292; EC=3.1.2.3;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11517;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glutaryl-CoA + H2O = CoA + glutarate + H(+);
CC Xref=Rhea:RHEA:40575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30921, ChEBI:CHEBI:57287, ChEBI:CHEBI:57378;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40576;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + hexanedioyl-CoA = CoA + H(+) + hexanedioate;
CC Xref=Rhea:RHEA:40583, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17128, ChEBI:CHEBI:57287, ChEBI:CHEBI:76327;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40584;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + octanedioyl-CoA = CoA + H(+) + octanedioate;
CC Xref=Rhea:RHEA:40587, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:76282, ChEBI:CHEBI:76317;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40588;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=decanedioyl-CoA + H2O = CoA + decanedioate + H(+);
CC Xref=Rhea:RHEA:40591, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:76283, ChEBI:CHEBI:76316;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40592;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dodecanedioyl-CoA + H2O = CoA + dodecanedioate + H(+);
CC Xref=Rhea:RHEA:40595, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:76273, ChEBI:CHEBI:76315;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40596;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-tetradecenoyl-CoA + H2O = (9Z)-tetradecenoate + CoA +
CC H(+); Xref=Rhea:RHEA:40135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:32370, ChEBI:CHEBI:57287, ChEBI:CHEBI:65060;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40136;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate;
CC Xref=Rhea:RHEA:16645, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379; EC=3.1.2.2;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16646;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-hexadecenoyl-CoA + H2O = (9Z)-hexadecenoate + CoA + H(+);
CC Xref=Rhea:RHEA:40131, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:32372, ChEBI:CHEBI:57287, ChEBI:CHEBI:61540;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40132;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + octadecanoyl-CoA = CoA + H(+) + octadecanoate;
CC Xref=Rhea:RHEA:30139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:25629, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30140;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+);
CC Xref=Rhea:RHEA:40139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30823, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40140;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + H2O = (9Z,12Z)-octadecadienoate
CC + CoA + H(+); Xref=Rhea:RHEA:40143, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30245, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57383; Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40144;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=eicosanoyl-CoA + H2O = CoA + eicosanoate + H(+);
CC Xref=Rhea:RHEA:40147, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:32360, ChEBI:CHEBI:57287, ChEBI:CHEBI:57380;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40148;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-
CC eicosatetraenoate + CoA + H(+); Xref=Rhea:RHEA:40151,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32395,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57368;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40152;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3S)-hydroxy-3-methylglutaryl-CoA + H2O = 3-hydroxy-3-
CC methylglutarate + CoA + H(+); Xref=Rhea:RHEA:16305,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17325,
CC ChEBI:CHEBI:43074, ChEBI:CHEBI:57287; EC=3.1.2.5;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16306;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA +
CC H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA
CC + H(+); Xref=Rhea:RHEA:59936, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:63001, ChEBI:CHEBI:85674;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59937;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-methyloctadecanoyl-CoA + H2O = 2-methyloctadecanoate + CoA +
CC H(+); Xref=Rhea:RHEA:59940, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:143530, ChEBI:CHEBI:143531;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59941;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + prostaglandin F2alpha-CoA = CoA + H(+) + prostaglandin
CC F2alpha; Xref=Rhea:RHEA:59948, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57404, ChEBI:CHEBI:143532;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59949;
CC Evidence={ECO:0000250|UniProtKB:P58137};
CC -!- ACTIVITY REGULATION: Inhibited by CoASH (IC(50)=10-15 uM). Also
CC inhibited by cysteine-reactive agents. {ECO:0000250|UniProtKB:P58137}.
CC -!- SUBUNIT: homodimer. {ECO:0000250|UniProtKB:P58137}.
CC -!- SUBCELLULAR LOCATION: Peroxisome matrix {ECO:0000269|PubMed:10092594,
CC ECO:0000269|PubMed:11785945}. Note=Predominantly localized in the
CC peroxisome but a localization to the cytosol cannot be excluded.
CC {ECO:0000269|PubMed:10092594, ECO:0000269|PubMed:11785945}.
CC -!- SIMILARITY: Belongs to the C/M/P thioester hydrolase family.
CC {ECO:0000305}.
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DR EMBL; AF452100; AAL66289.1; -; mRNA.
DR AlphaFoldDB; Q8VHK0; -.
DR SMR; Q8VHK0; -.
DR STRING; 10116.ENSRNOP00000020740; -.
DR SwissLipids; SLP:000001955; -.
DR iPTMnet; Q8VHK0; -.
DR PhosphoSitePlus; Q8VHK0; -.
DR jPOST; Q8VHK0; -.
DR PaxDb; Q8VHK0; -.
DR PRIDE; Q8VHK0; -.
DR UCSC; RGD:70368; rat.
DR RGD; 70368; Acot8.
DR eggNOG; KOG3016; Eukaryota.
DR InParanoid; Q8VHK0; -.
DR PhylomeDB; Q8VHK0; -.
DR BRENDA; 3.1.2.2; 5301.
DR BRENDA; 3.1.2.20; 5301.
DR Reactome; R-RNO-193368; Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
DR Reactome; R-RNO-2046106; alpha-linolenic acid (ALA) metabolism.
DR Reactome; R-RNO-389887; Beta-oxidation of pristanoyl-CoA.
DR Reactome; R-RNO-390247; Beta-oxidation of very long chain fatty acids.
DR Reactome; R-RNO-9033241; Peroxisomal protein import.
DR PRO; PR:Q8VHK0; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005782; C:peroxisomal matrix; ISO:RGD.
DR GO; GO:0005777; C:peroxisome; IDA:UniProtKB.
DR GO; GO:0047603; F:acetoacetyl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0003986; F:acetyl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0047617; F:acyl-CoA hydrolase activity; IDA:UniProtKB.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0033882; F:choloyl-CoA hydrolase activity; IDA:UniProtKB.
DR GO; GO:0044466; F:glutaryl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0047994; F:hydroxymethylglutaryl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0052815; F:medium-chain acyl-CoA hydrolase activity; ISS:UniProtKB.
DR GO; GO:0102991; F:myristoyl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0016290; F:palmitoyl-CoA hydrolase activity; ISS:UniProtKB.
DR GO; GO:0004778; F:succinyl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0006637; P:acyl-CoA metabolic process; IDA:UniProtKB.
DR GO; GO:0043649; P:dicarboxylic acid catabolic process; ISO:RGD.
DR GO; GO:0009062; P:fatty acid catabolic process; IBA:GO_Central.
DR GO; GO:0045225; P:negative regulation of CD4 production; ISS:UniProtKB.
DR GO; GO:0016559; P:peroxisome fission; ISO:RGD.
DR Gene3D; 2.40.160.210; -; 1.
DR InterPro; IPR042171; Acyl-CoA_hotdog.
DR InterPro; IPR003703; Acyl_CoA_thio.
DR InterPro; IPR029069; HotDog_dom_sf.
DR PANTHER; PTHR11066; PTHR11066; 1.
DR SUPFAM; SSF54637; SSF54637; 2.
DR TIGRFAMs; TIGR00189; tesB; 1.
PE 1: Evidence at protein level;
KW Fatty acid metabolism; Hydrolase; Lipid metabolism; Peroxisome;
KW Peroxisome biogenesis; Reference proteome; Serine esterase.
FT CHAIN 1..320
FT /note="Acyl-coenzyme A thioesterase 8"
FT /id="PRO_0000202154"
FT MOTIF 318..320
FT /note="Microbody targeting signal"
FT /evidence="ECO:0000255"
FT ACT_SITE 233
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 255
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 305
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
SQ SEQUENCE 320 AA; 36003 MW; E0EAB4B7D26C79C0 CRC64;
MSKPEDLGDA NGDADRGDLS GDLRSVLVTS VLNLEPLDED LYRGRHYWVP TSQRLFGGQI
VGQALVAAAK SVSEDVHVHS LHCYFVRAGD PKVPVLYHVE RTRTGASFSV RAVKAVQHGK
AIFICQASFQ QMQPSPLQHQ FSMPTVPPPE ELLDHEALID QYLRDPNLHE KYRVGLNRIA
AREVPIEIKL VNPPALNQLQ TLEPKQMFWV RARGYIGEGD IKMHCCVAAY ISDYAFLGTA
LLPHQSKYKV NFMVSLDHSM WFHAPFRADH WMLYECESPW AGGSRGLVHG RLWRRDGVLA
VTCAQEGVIR SKPRVSESKL
