ID   COBQ_STRCO              Reviewed;         502 AA.
AC   Q9RJ20;
DT   23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; OrderedLocusNames=SCO1848;
GN   ORFNames=SCI8.33;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR   EMBL; AL939110; CAB59464.1; -; Genomic_DNA.
DR   RefSeq; NP_626116.1; NC_003888.3.
DR   RefSeq; WP_011028007.1; NZ_VNID01000001.1.
DR   AlphaFoldDB; Q9RJ20; -.
DR   STRING; 100226.SCO1848; -.
DR   GeneID; 1097282; -.
DR   KEGG; sco:SCO1848; -.
DR   PATRIC; fig|100226.15.peg.1872; -.
DR   eggNOG; COG1492; Bacteria.
DR   HOGENOM; CLU_019250_2_2_11; -.
DR   InParanoid; Q9RJ20; -.
DR   OMA; EIHHGVA; -.
DR   PhylomeDB; Q9RJ20; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..502
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_0000141332"
FT   DOMAIN          260..433
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        341
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        425
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   502 AA;  53505 MW;  0E01E4B972EDC110 CRC64;
     MNGGLLVAGT TSDAGKSVVT AGICRWLVRQ GVKVAPFKAQ NMSLNSFVTR DGAEIGRAQA
     MQAQACRVEP TAHMNPVLLK PGGERSSQVV LLGRPVGEMS ARGYHGGRQE RLLGTVLDSL
     AELRGTYDAV ICEGAGSPAE INLRRTDIVN MGIARGAGLP VLVVGDIDRG GVFASFFGTV
     ALLAPEDQAL VAGFLVNKFR GDVSLLEPGL DMLHGLTGRR TYGVLPFRHG LGIDEEDGLR
     VSLRGTVRES VVAPPVGEDV LRVAVCAVPL MSNFTDVDAL AAEPGVVVRF VDRPEELADA
     DLVIVPGTRG TVRALEWLRE RGLADAIARR AAERRPVLGI CGGYQLLGEH IEDEVESRAG
     HVDGLGLLPV RVRFDREKTL TRAVGEALGE PVEGYEIHHG VAEVTGGAPF LDGCRVGRTW
     GTHWHGSLES DGFRRAFLRE VAAAAGRRFV PAPDTSFAAL REEQLDRLGD LIEHHADTDA
     LWRLIESGAP QGLPFIPPGA PA