ID   COBQ_SYNY3              Reviewed;         498 AA.
AC   Q55860;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   25-MAY-2022, entry version 126.
DE   RecName: Full=Cobyric acid synthase;
GN   Name=cobQ; Synonyms=cbiP; OrderedLocusNames=slr0618;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX   PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA   Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA   Sugiura M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region
RT   from map positions 64% to 92% of the genome.";
RL   DNA Res. 2:153-166(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation (By
CC       similarity). {ECO:0000250}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA10617.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BA000022; BAA10617.1; ALT_INIT; Genomic_DNA.
DR   PIR; S76673; S76673.
DR   AlphaFoldDB; Q55860; -.
DR   SMR; Q55860; -.
DR   IntAct; Q55860; 1.
DR   STRING; 1148.1001778; -.
DR   PaxDb; Q55860; -.
DR   EnsemblBacteria; BAA10617; BAA10617; BAA10617.
DR   KEGG; syn:slr0618; -.
DR   eggNOG; COG1492; Bacteria.
DR   InParanoid; Q55860; -.
DR   OMA; EIHHGVA; -.
DR   PhylomeDB; Q55860; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..498
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_0000141336"
FT   DOMAIN          250..439
FT                   /note="GATase cobBQ-type"
FT   ACT_SITE        331
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        431
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   498 AA;  54813 MW;  AA265C71E2829299 CRC64;
     MKAIMVVGTT SHAGKSFLTT GLCRLLRRRG DRVTPFKGQN MALNAFVTID GGEMGHAQAV
     QAWAAGTVPR VAMNPILLKP QGDMTSQVIM LGQAVGTTRA QEYYQNYFDR GWQAIANSLE
     TLAQDFDVVV CEGAGSPAEI NLKHRDLTNM RVATHLQAAT ILIADIDRGG VFAHVVGTLQ
     LLEPEERKLI KGIVINKFRG QRSLLDSGIT WLEEYTGIPV LGVIPYGQNL FSAEDSLDIL
     ERSGRPSKED IRIVVLRLPR IANFTDFEPL EAESGVYVDY LPLEKQLGNP DAVIIPGSKT
     TIADLQALQN SGMAQQIMDY AQAGGTVMGI CGGYQMLGQT IADPENIEGN VSNCQGLGLL
     PLTTVINQEK ITRQCQTITH APPSGLTVTG YEIHQGLSRR SEHSQDFLPM FDQEELGMVN
     AQQNLWGCYL HGLFDNGAWR RCWLNNLRQR RGLDPLPTSI GNYQEQREGM LDSLADFVET
     HVNLEPILQS LPEENETP