ID   COBQ_XANP2              Reviewed;         488 AA.
AC   A7ILW0;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; OrderedLocusNames=Xaut_3778;
OS   Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Xanthobacteraceae; Xanthobacter.
OX   NCBI_TaxID=78245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1158 / Py2;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N.,
RA   Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D.,
RA   Detter J.C., Han C., Tapia R., Brainard J., Schmutz J., Larimer F.,
RA   Land M., Hauser L., Kyrpides N., Kim E., Ensigns S.A., Richardson P.;
RT   "Complete sequence of chromosome of Xanthobacter autotrophicus Py2.";
RL   Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR   EMBL; CP000781; ABS69003.1; -; Genomic_DNA.
DR   RefSeq; WP_012115750.1; NC_009720.1.
DR   AlphaFoldDB; A7ILW0; -.
DR   SMR; A7ILW0; -.
DR   STRING; 78245.Xaut_3778; -.
DR   PRIDE; A7ILW0; -.
DR   EnsemblBacteria; ABS69003; ABS69003; Xaut_3778.
DR   KEGG; xau:Xaut_3778; -.
DR   eggNOG; COG1492; Bacteria.
DR   HOGENOM; CLU_019250_2_2_5; -.
DR   OMA; EIHHGVA; -.
DR   OrthoDB; 744477at2; -.
DR   PhylomeDB; A7ILW0; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000002417; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..488
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_1000090254"
FT   DOMAIN          252..442
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        334
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        434
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   488 AA;  50840 MW;  DD055DEE2C6ED5DF CRC64;
     MARALMFQGT GSDVGKSLLV AGLARAFTLR GLKVRPFKPQ NMSNNAAVTA DGGEIGRAQA
     LQARAARVPL SVHMNPVLLK PQGETGAQVV VQGRVHGTAK AAAYQGMKPS LLPFVLDSFD
     RLKAEADLVL VEGAGSASEV NLRTGDIANM GFARAADVPV VVIGDIDRGG VIASLVGTKA
     VIDAADAALI KGFVVNRFRG DPSLFATGME LIARQTGWAA LGLVPHFSEA IRLPAEDALA
     LSAPPAPKPR ARTRICVPIL PHVSNFDDLD PLDAEPSVEV RRIRPHETLP VDTDLVLLIG
     SKATIADLAA LKAEGLHHDI LAFARRGGHV MGLCGGYQML GETIADPDGI EGEAKIARGL
     GLLKVHTVLS PEKRLVEVAG VALDPAVAAP FSGYEMHMGV TAGADAEQPF AVLSDGRQDG
     ARSASGRVSG TYVHGLFASD AFRSGLLGAL GGAPSQAAYE QGVEETLDRL AAHLAAHLDL
     DLLFSLAR