COBRA_ARATH
ID COBRA_ARATH Reviewed; 456 AA.
AC Q94KT8; O81811; Q8LDZ4;
DT 22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=Protein COBRA;
DE AltName: Full=Cell expansion protein;
DE Flags: Precursor;
GN Name=COB; OrderedLocusNames=At5g60920; ORFNames=MSL3.40;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RX PubMed=11331607; DOI=10.1101/gad.879101;
RA Schindelman G., Morikami A., Jung J., Baskin T.I., Carpita N.C.,
RA Derbyshire P., McCann M.C., Benfey P.N.;
RT "COBRA encodes a putative GPI-anchored protein, which is polarly localized
RT and necessary for oriented cell expansion in Arabidopsis.";
RL Genes Dev. 15:1115-1127(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 62-456.
RA Leuchter R., Wolf K., Zimmermann M.;
RT "Isolation of an Arabidopsis thaliana cDNA complementing a
RT Schizosaccharomyces pombe mutant which is deficient in phytochelatin
RT synthesis.";
RL (er) Plant Gene Register PGR98-147(1998).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=12376623; DOI=10.1104/pp.007468;
RA Roudier F., Schindelman G., DeSalle R., Benfey P.N.;
RT "The COBRA family of putative GPI-anchored proteins in Arabidopsis. A new
RT fellowship in expansion.";
RL Plant Physiol. 130:538-548(2002).
CC -!- FUNCTION: Involved in determining the orientation of cell expansion,
CC probably by playing an important role in cellulose deposition. May act
CC by recruiting cellulose synthesizing complexes to discrete positions on
CC the cell surface.
CC -!- SUBCELLULAR LOCATION: Lateral cell membrane
CC {ECO:0000305|PubMed:11331607}; Lipid-anchor, GPI-anchor
CC {ECO:0000305|PubMed:11331607}; Extracellular side
CC {ECO:0000305|PubMed:11331607}. Note=Located on the longitudinal sides
CC of the cell rather than on the apical or basal sides.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, flowers and
CC siliques. Up-regulated in the root zone of rapid longitudinal
CC expansion. {ECO:0000269|PubMed:11331607, ECO:0000269|PubMed:12376623}.
CC -!- MISCELLANEOUS: A partial protein missing the N-terminal signal peptide
CC was reported to complement a yeast mutant defective in phytochelatin
CC synthesis. It is therefore possible that COBRA binds divalent metals.
CC -!- SIMILARITY: Belongs to the COBRA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10641.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAA07251.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF319663; AAK56072.1; -; Genomic_DNA.
DR EMBL; AB008269; BAB10641.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97397.1; -; Genomic_DNA.
DR EMBL; AY094402; AAM19781.1; -; mRNA.
DR EMBL; BT001119; AAN64510.1; -; mRNA.
DR EMBL; AY085712; AAM62930.1; -; mRNA.
DR EMBL; AJ006787; CAA07251.1; ALT_INIT; mRNA.
DR PIR; T52038; T52038.
DR RefSeq; NP_568930.1; NM_125485.4.
DR AlphaFoldDB; Q94KT8; -.
DR BioGRID; 21457; 1.
DR STRING; 3702.AT5G60920.1; -.
DR PaxDb; Q94KT8; -.
DR PRIDE; Q94KT8; -.
DR ProteomicsDB; 241141; -.
DR EnsemblPlants; AT5G60920.1; AT5G60920.1; AT5G60920.
DR GeneID; 836213; -.
DR Gramene; AT5G60920.1; AT5G60920.1; AT5G60920.
DR KEGG; ath:AT5G60920; -.
DR Araport; AT5G60920; -.
DR TAIR; locus:2173532; AT5G60920.
DR eggNOG; ENOG502QTGW; Eukaryota.
DR HOGENOM; CLU_038120_0_0_1; -.
DR InParanoid; Q94KT8; -.
DR OMA; IVFLWAY; -.
DR OrthoDB; 494933at2759; -.
DR PhylomeDB; Q94KT8; -.
DR PRO; PR:Q94KT8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q94KT8; baseline and differential.
DR Genevisible; Q94KT8; AT.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0046658; C:anchored component of plasma membrane; IDA:TAIR.
DR GO; GO:0009897; C:external side of plasma membrane; ISS:TAIR.
DR GO; GO:0016328; C:lateral plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009930; C:longitudinal side of cell surface; IDA:TAIR.
DR GO; GO:0009505; C:plant-type cell wall; IDA:TAIR.
DR GO; GO:0010215; P:cellulose microfibril organization; IMP:TAIR.
DR GO; GO:0009825; P:multidimensional cell growth; IMP:TAIR.
DR GO; GO:0052324; P:plant-type cell wall cellulose biosynthetic process; IBA:GO_Central.
DR GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR InterPro; IPR006918; COBRA_pln.
DR PANTHER; PTHR31673; PTHR31673; 1.
DR Pfam; PF04833; COBRA; 1.
DR PIRSF; PIRSF038122; COBRA; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Signal.
FT SIGNAL 1..36
FT /evidence="ECO:0000255"
FT CHAIN 37..431
FT /note="Protein COBRA"
FT /id="PRO_0000005570"
FT PROPEP 432..456
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000005571"
FT LIPID 431
FT /note="GPI-anchor amidated asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 170
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 178
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 217
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 242
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 258
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 328
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 343
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 362
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 456 AA; 51203 MW; 9868B40C2E76D2C3 CRC64;
MESFFSRSTS IVSKLSFLAL WIVFLISSSS FTSTEAYDAL DPEGNITMKW DVMSWTPDGY
VAVVTMFNFQ KYRHIQSPGW TLGWKWAKKE VIWSMVGAQT TEQGDCSKYK GNIPHCCKKD
PTVVDLLPGT PYNQQIANCC KGGVMNSWVQ DPATAASSFQ ISVGAAGTTN KTVRVPRNFT
LMGPGPGYTC GPAKIVRPTK FVTTDTRRTT QAMMTWNITC TYSQFLAQRT PTCCVSLSSF
YNETIVGCPT CACGCQNNRT ESGACLDPDT PHLASVVSPP TKKGTVLPPL VQCTRHMCPI
RVHWHVKQNY KEYWRVKITI TNFNYRLNYT QWNLVAQHPN LDNITQIFSF NYKSLTPYAG
LNDTAMLWGV KFYNDFLSEA GPLGNVQSEI LFRKDQSTFT FEKGWAFPRR IYFNGDNCVM
PPPDSYPFLP NGGSRSQFSF VAAVLLPLLV FFFFSA
