COBS_ECOLI
ID COBS_ECOLI Reviewed; 247 AA.
AC P36561; P76354;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 3.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Adenosylcobinamide-GDP ribazoletransferase;
DE EC=2.7.8.26;
DE AltName: Full=Cobalamin synthase;
DE AltName: Full=Cobalamin-5'-phosphate synthase;
GN Name=cobS; OrderedLocusNames=b1992, JW1970;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=7592411; DOI=10.1128/jb.177.22.6371-6380.1995;
RA Lawrence J.G., Roth J.R.;
RT "The cobalamin (coenzyme B12) biosynthetic genes of Escherichia coli.";
RL J. Bacteriol. 177:6371-6380(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-247.
RC STRAIN=K12 / DH5-alpha;
RA Collins C.M., Gutman D.M., Isaza J.;
RT "Location of cobS and cobT gene sequences on the physical map of the
RT Escherichia coli K-12 chromosome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Joins adenosylcobinamide-GDP and alpha-ribazole to generate
CC adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin
CC 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-
CC phosphate (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=adenosylcob(III)inamide-GDP + alpha-ribazole =
CC adenosylcob(III)alamin + GMP + H(+); Xref=Rhea:RHEA:16049,
CC ChEBI:CHEBI:10329, ChEBI:CHEBI:15378, ChEBI:CHEBI:18408,
CC ChEBI:CHEBI:58115, ChEBI:CHEBI:60487; EC=2.7.8.26;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=adenosylcob(III)inamide-GDP + alpha-ribazole 5'-phosphate =
CC adenosylcob(III)alamin 5'-phosphate + GMP + H(+);
CC Xref=Rhea:RHEA:23560, ChEBI:CHEBI:15378, ChEBI:CHEBI:57918,
CC ChEBI:CHEBI:58115, ChEBI:CHEBI:60487, ChEBI:CHEBI:60493; EC=2.7.8.26;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
CC -!- INDUCTION: By cobinamide.
CC -!- SIMILARITY: Belongs to the CobS family. {ECO:0000305}.
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DR EMBL; U33333; AAA78907.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75053.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15809.1; -; Genomic_DNA.
DR EMBL; L25054; AAA56875.1; -; Genomic_DNA.
DR PIR; G64963; G64963.
DR RefSeq; NP_416496.1; NC_000913.3.
DR RefSeq; WP_001326708.1; NZ_SSUV01000031.1.
DR AlphaFoldDB; P36561; -.
DR BioGRID; 4260409; 10.
DR IntAct; P36561; 5.
DR STRING; 511145.b1992; -.
DR PaxDb; P36561; -.
DR PRIDE; P36561; -.
DR EnsemblBacteria; AAC75053; AAC75053; b1992.
DR EnsemblBacteria; BAA15809; BAA15809; BAA15809.
DR GeneID; 946520; -.
DR KEGG; ecj:JW1970; -.
DR KEGG; eco:b1992; -.
DR PATRIC; fig|1411691.4.peg.262; -.
DR EchoBASE; EB2070; -.
DR eggNOG; COG0368; Bacteria.
DR HOGENOM; CLU_057426_1_1_6; -.
DR InParanoid; P36561; -.
DR OMA; GHTGDTY; -.
DR PhylomeDB; P36561; -.
DR BioCyc; EcoCyc:COBS-MON; -.
DR BioCyc; MetaCyc:COBS-MON; -.
DR UniPathway; UPA00148; UER00238.
DR PRO; PR:P36561; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0051073; F:adenosylcobinamide-GDP ribazoletransferase activity; IBA:GO_Central.
DR GO; GO:0008818; F:cobalamin 5'-phosphate synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IMP:EcoliWiki.
DR HAMAP; MF_00719; CobS; 1.
DR InterPro; IPR003805; CobS.
DR PANTHER; PTHR34148; PTHR34148; 1.
DR Pfam; PF02654; CobS; 1.
DR TIGRFAMs; TIGR00317; cobS; 1.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Cobalamin biosynthesis; Magnesium;
KW Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..247
FT /note="Adenosylcobinamide-GDP ribazoletransferase"
FT /id="PRO_0000146876"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 48..49
FT /note="SG -> RR (in Ref. 1 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 145
FT /note="A -> R (in Ref. 5; AAA56875)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="A -> T (in Ref. 5; AAA56875)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 247 AA; 26386 MW; 8D48B8C91428F13F CRC64;
MSKLFWAMLS FITRLPVPRR WSQGLDFEHY SRGIITFPLI GLLLGAISGL VFMVLQAWCG
APLAALFSVL VLVLMTGGFH LDGLADTCDG VFSARSRDRM LEIMRDSRLG THGGLALIFV
VLAKILVLSE LALRGESILA SLAAACAVSR GTAALLMYRH RYAREEGLGN VFIGKIDGRQ
TCVTLGLAAI FAAVLLPGMH GVAAMVVTMV AIFILGQLLK RTLGGQTGDT LGAAIELGEL
VFLLALL
