COBT_ECOLI
ID COBT_ECOLI Reviewed; 359 AA.
AC P36562; P78075;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 3.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase;
DE Short=NN:DBI PRT;
DE EC=2.4.2.21;
DE AltName: Full=N(1)-alpha-phosphoribosyltransferase;
GN Name=cobT; OrderedLocusNames=b1991, JW1969;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=7592411; DOI=10.1128/jb.177.22.6371-6380.1995;
RA Lawrence J.G., Roth J.R.;
RT "The cobalamin (coenzyme B12) biosynthetic genes of Escherichia coli.";
RL J. Bacteriol. 177:6371-6380(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-188.
RC STRAIN=K12 / DH5-alpha;
RA Collins C.M., Gutman D.M., Isaza J.;
RT "Location of cobS and cobT gene sequences on the physical map of the
RT Escherichia coli K-12 chromosome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the synthesis of alpha-ribazole-5'-phosphate from
CC nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,6-dimethylbenzimidazole + nicotinate beta-D-ribonucleotide =
CC alpha-ribazole 5'-phosphate + H(+) + nicotinate;
CC Xref=Rhea:RHEA:11196, ChEBI:CHEBI:15378, ChEBI:CHEBI:15890,
CC ChEBI:CHEBI:32544, ChEBI:CHEBI:57502, ChEBI:CHEBI:57918; EC=2.4.2.21;
CC -!- PATHWAY: Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-
CC ribazole from 5,6-dimethylbenzimidazole: step 1/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CobT family. {ECO:0000305}.
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DR EMBL; U33333; AAA78908.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75052.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15808.1; -; Genomic_DNA.
DR EMBL; L25054; AAA56876.1; -; Genomic_DNA.
DR PIR; F64963; F64963.
DR RefSeq; NP_416495.1; NC_000913.3.
DR RefSeq; WP_001166160.1; NZ_SSUV01000031.1.
DR AlphaFoldDB; P36562; -.
DR SMR; P36562; -.
DR BioGRID; 4262028; 15.
DR IntAct; P36562; 5.
DR STRING; 511145.b1991; -.
DR jPOST; P36562; -.
DR PaxDb; P36562; -.
DR PRIDE; P36562; -.
DR EnsemblBacteria; AAC75052; AAC75052; b1991.
DR EnsemblBacteria; BAA15808; BAA15808; BAA15808.
DR GeneID; 946517; -.
DR KEGG; ecj:JW1969; -.
DR KEGG; eco:b1991; -.
DR PATRIC; fig|1411691.4.peg.263; -.
DR EchoBASE; EB2071; -.
DR eggNOG; COG2038; Bacteria.
DR HOGENOM; CLU_002982_0_0_6; -.
DR InParanoid; P36562; -.
DR OMA; AIWYAGW; -.
DR PhylomeDB; P36562; -.
DR BioCyc; EcoCyc:DMBPPRIBOSYLTRANS-MON; -.
DR UniPathway; UPA00061; UER00516.
DR PRO; PR:P36562; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0008939; F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IMP:EcoliWiki.
DR CDD; cd02439; DMB-PRT_CobT; 1.
DR Gene3D; 1.10.1610.10; -; 1.
DR Gene3D; 3.40.50.10210; -; 1.
DR HAMAP; MF_00230; CobT; 1.
DR InterPro; IPR003200; Nict_dMeBzImd_PRibTrfase.
DR InterPro; IPR017846; Nict_dMeBzImd_PRibTrfase_bact.
DR InterPro; IPR023195; Nict_dMeBzImd_PRibTrfase_N.
DR InterPro; IPR036087; Nict_dMeBzImd_PRibTrfase_sf.
DR Pfam; PF02277; DBI_PRT; 1.
DR SUPFAM; SSF52733; SSF52733; 1.
DR TIGRFAMs; TIGR03160; cobT_DBIPRT; 1.
PE 3: Inferred from homology;
KW Cobalamin biosynthesis; Glycosyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..359
FT /note="Nicotinate-nucleotide--dimethylbenzimidazole
FT phosphoribosyltransferase"
FT /id="PRO_0000167048"
FT ACT_SITE 318
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT CONFLICT 271
FT /note="A -> L (in Ref. 1; AAA78908)"
FT /evidence="ECO:0000305"
FT CONFLICT 301
FT /note="L -> V (in Ref. 1; AAA78908)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 359 AA; 36987 MW; 6058C6465FAE55EF CRC64;
MQILADLLNT IPAIDSTAMS RAQRHIDGLL KPVGSLGKLE VLAIQLAGMP GLNGIPHVGK
KAVLVMCADH GVWEEGVAIS PKEVTAIQAE NMTRGTTGVC VLAEQAGANV HVIDVGIDTA
EPIPGLINMR VARGSGNIAS APAMSRRQAE KLLLDVICYT QELAKNGVTL FGVGELGMAN
TTPAAAIVST ITGRDPEEVV GIGANLPTDK LANKIDVVRR AITLNQPNPQ DGVDVLAKVG
GFDLVGIAGV MLGAASCGLP VLLDGFLSYA AALAACQMSP AIKPYLIPSH LSAEKGARIA
LSHLGLEPYL NMEMRLGEGS GAALAMPIIE AACAIYNNMG ELAASNIVLP GNTTSDLNS
