ID   COBT_METC4              Reviewed;         342 AA.
AC   Q9X7F4; B7L3L2;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase;
DE            Short=NN:DBI PRT;
DE            EC=2.4.2.21;
DE   AltName: Full=N(1)-alpha-phosphoribosyltransferase;
GN   Name=cobT; Synonyms=cobU; OrderedLocusNames=Mchl_5702;
OS   Methylorubrum extorquens (strain CM4 / NCIMB 13688) (Methylobacterium
OS   extorquens).
OG   Plasmid pMCHL01.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylorubrum.
OX   NCBI_TaxID=440085;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10200311; DOI=10.1073/pnas.96.8.4615;
RA   Vannelli T., Messmer M., Studer A., Vuilleumier S., Leisinger T.;
RT   "A corrinoid-dependent catabolic pathway for growth of a Methylobacterium
RT   strain with chloromethane.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:4615-4620(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CM4 / NCIMB 13688;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C.,
RA   Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA   Marx C., Richardson P.;
RT   "Complete sequence of plasmid1 of Methylobacterium chloromethanicum CM4.";
RL   Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the synthesis of alpha-ribazole-5'-phosphate from
CC       nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dimethylbenzimidazole + nicotinate beta-D-ribonucleotide =
CC         alpha-ribazole 5'-phosphate + H(+) + nicotinate;
CC         Xref=Rhea:RHEA:11196, ChEBI:CHEBI:15378, ChEBI:CHEBI:15890,
CC         ChEBI:CHEBI:32544, ChEBI:CHEBI:57502, ChEBI:CHEBI:57918; EC=2.4.2.21;
CC   -!- PATHWAY: Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-
CC       ribazole from 5,6-dimethylbenzimidazole: step 1/2.
CC   -!- SIMILARITY: Belongs to the CobT family. {ECO:0000305}.
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DR   EMBL; AJ011316; CAB39398.1; -; Genomic_DNA.
DR   EMBL; CP001299; ACK86420.1; -; Genomic_DNA.
DR   PIR; T51703; T51703.
DR   RefSeq; WP_012606310.1; NC_011758.1.
DR   AlphaFoldDB; Q9X7F4; -.
DR   SMR; Q9X7F4; -.
DR   EnsemblBacteria; ACK86420; ACK86420; Mchl_5702.
DR   KEGG; mch:Mchl_5702; -.
DR   HOGENOM; CLU_002982_0_1_5; -.
DR   BioCyc; MEXT440085:MCHL_RS27755-MON; -.
DR   UniPathway; UPA00061; UER00516.
DR   Proteomes; UP000002385; Plasmid pCMU01.
DR   GO; GO:0008939; F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd02439; DMB-PRT_CobT; 1.
DR   Gene3D; 1.10.1610.10; -; 1.
DR   Gene3D; 3.40.50.10210; -; 1.
DR   HAMAP; MF_00230; CobT; 1.
DR   InterPro; IPR003200; Nict_dMeBzImd_PRibTrfase.
DR   InterPro; IPR017846; Nict_dMeBzImd_PRibTrfase_bact.
DR   InterPro; IPR023195; Nict_dMeBzImd_PRibTrfase_N.
DR   InterPro; IPR036087; Nict_dMeBzImd_PRibTrfase_sf.
DR   Pfam; PF02277; DBI_PRT; 1.
DR   SUPFAM; SSF52733; SSF52733; 1.
DR   TIGRFAMs; TIGR03160; cobT_DBIPRT; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glycosyltransferase; Plasmid; Transferase.
FT   CHAIN           1..342
FT                   /note="Nicotinate-nucleotide--dimethylbenzimidazole
FT                   phosphoribosyltransferase"
FT                   /id="PRO_0000167054"
FT   ACT_SITE        308
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   342 AA;  34913 MW;  0819BB838D519223 CRC64;
     MGEIGGNALD GIHARVRTAP LADQAAIAAV RQRDRLLTKP PGSLGRLEAI VEWLAAWQGK
     SPPVLESAAA VVFAGNHGVA KHGVSAYPAE VTAQMVANFE AGGAAINQIA KANGLKLSIV
     PIDLDRPTAD IIIADAMSVQ ECAAAVNLGA SVAAADLDLL CIGEMGIGNT TPASAILQAL
     FGGPAHIWVG PGTGVERDAL TNKIRAVESA VARVIGTDGT SPLEFLRRLG GREIAALVGA
     IIEARHRRIP VLIDGFVATA AAAVVHAIEP GAIDHCLIGH LSAEPAHRRA VQALGKTPIL
     ELNMRLGEAS GAALAAGIVR SALAVHTGMA TFAEAGVTDR QT