ID   COBT_RHOCB              Reviewed;         334 AA.
AC   D5AV20; O68087; Q52679;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase;
DE            Short=NN:DBI PRT;
DE            EC=2.4.2.21;
DE   AltName: Full=N(1)-alpha-phosphoribosyltransferase;
GN   Name=cobT; Synonyms=cobU; OrderedLocusNames=RCAP_rcc02058;
OS   Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=272942;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX   PubMed=9256491; DOI=10.1073/pnas.94.17.9384;
RA   Vlcek C., Paces V., Maltsev N., Paces J., Haselkorn R., Fonstein M.;
RT   "Sequence of a 189-kb segment of the chromosome of Rhodobacter capsulatus
RT   SB1003.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:9384-9388(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX   PubMed=20418398; DOI=10.1128/jb.00366-10;
RA   Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V.,
RA   Haselkorn R.;
RT   "Complete genome sequence of the photosynthetic purple nonsulfur bacterium
RT   Rhodobacter capsulatus SB 1003.";
RL   J. Bacteriol. 192:3545-3546(2010).
CC   -!- FUNCTION: Catalyzes the synthesis of alpha-ribazole-5'-phosphate from
CC       nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dimethylbenzimidazole + nicotinate beta-D-ribonucleotide =
CC         alpha-ribazole 5'-phosphate + H(+) + nicotinate;
CC         Xref=Rhea:RHEA:11196, ChEBI:CHEBI:15378, ChEBI:CHEBI:15890,
CC         ChEBI:CHEBI:32544, ChEBI:CHEBI:57502, ChEBI:CHEBI:57918; EC=2.4.2.21;
CC   -!- PATHWAY: Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-
CC       ribazole from 5,6-dimethylbenzimidazole: step 1/2.
CC   -!- SIMILARITY: Belongs to the CobT family. {ECO:0000305}.
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DR   EMBL; AF010496; AAC16173.1; -; Genomic_DNA.
DR   EMBL; CP001312; ADE85802.1; -; Genomic_DNA.
DR   PIR; T03520; T03520.
DR   RefSeq; WP_013067781.1; NC_014034.1.
DR   AlphaFoldDB; D5AV20; -.
DR   SMR; D5AV20; -.
DR   STRING; 272942.RCAP_rcc02058; -.
DR   EnsemblBacteria; ADE85802; ADE85802; RCAP_rcc02058.
DR   GeneID; 31490920; -.
DR   KEGG; rcp:RCAP_rcc02058; -.
DR   eggNOG; COG2038; Bacteria.
DR   HOGENOM; CLU_002982_0_1_5; -.
DR   OMA; AIWYAGW; -.
DR   OrthoDB; 1765140at2; -.
DR   UniPathway; UPA00061; UER00516.
DR   Proteomes; UP000002361; Chromosome.
DR   GO; GO:0008939; F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd02439; DMB-PRT_CobT; 1.
DR   Gene3D; 1.10.1610.10; -; 1.
DR   Gene3D; 3.40.50.10210; -; 1.
DR   HAMAP; MF_00230; CobT; 1.
DR   InterPro; IPR003200; Nict_dMeBzImd_PRibTrfase.
DR   InterPro; IPR017846; Nict_dMeBzImd_PRibTrfase_bact.
DR   InterPro; IPR023195; Nict_dMeBzImd_PRibTrfase_N.
DR   InterPro; IPR036087; Nict_dMeBzImd_PRibTrfase_sf.
DR   Pfam; PF02277; DBI_PRT; 1.
DR   SUPFAM; SSF52733; SSF52733; 1.
DR   TIGRFAMs; TIGR03160; cobT_DBIPRT; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glycosyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..334
FT                   /note="Nicotinate-nucleotide--dimethylbenzimidazole
FT                   phosphoribosyltransferase"
FT                   /id="PRO_0000409931"
FT   ACT_SITE        303
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   334 AA;  34096 MW;  356A74BDEA623637 CRC64;
     MLQLNSFAEI STLADRLPVA DEAARAAALA RQNSLTKPVG SLGRLEELAL HWAAWRATER
     PTITKAQALC FAGNHGVCAQ GVNVFPQEVT HLMVKNFEMG GAAINQLCRA AGADLQVISL
     DLDRPTQDFT QGPAMTEAET VAALNVGAGA VDVTADALII GEMGIGNSTI AAALAAAIYG
     GTAKDFVGPG TGSDAAGIAR KIAAIDKGLA LHGGLGGMET LAALGGREIA AMCGAVLGAR
     AARIPVILDG FISTAAVSTL FKVDPRLLDH CVVGHTSAEP GHQKVLTTMG KRALLQMEMR
     LGEGTGAALA LGVVRAALEC HNGMVTYAEA GITA