ID   ACP1_CYCRE              Reviewed;           9 AA.
AC   B3EWE7;
DT   21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 1.
DT   11-DEC-2019, entry version 5.
DE   RecName: Full=Anticancerous peptide 1 {ECO:0000303|PubMed:21882228};
DE            Short=Cr-ACP1 {ECO:0000303|PubMed:21882228};
OS   Cycas revoluta (Sago palm).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Cycadidae; Cycadales; Cycadaceae; Cycas.
OX   NCBI_TaxID=3396;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SYNTHESIS, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Seed {ECO:0000269|PubMed:21882228};
RX   PubMed=21882228; DOI=10.1002/jcb.23343;
RA   Mandal S.M., Migliolo L., Das S., Mandal M., Franco O.L., Hazra T.K.;
RT   "Identification and characterization of a bactericidal and proapoptotic
RT   peptide from cycas revoluta seeds with DNA binding properties.";
RL   J. Cell. Biochem. 113:184-193(2012).
CC   -!- FUNCTION: The synthetic peptide inhibits cell proliferation and induces
CC       apoptosis in cancer-derived cell lines Hep2 (IC(50)=1.5 mM) and HCT15
CC       and, to a lesser extent, in non-cancerous NIH/3T3 cells. The mode of
CC       action is presumably an arrest in the G0/G1 phase. Has DNA-binding
CC       activity. Antiproliferative, proapoptotic and DNA-binding activities
CC       are increased by acetylation at Ala-1 and Lys-3. Has no hemolytic
CC       activity against mouse erythrocytes. Has antibacterial activity against
CC       S.epidermitis (MIC=60 ug), B.subtilis (MIC=30 ug), P.aeruginosa (MIC=30
CC       ug) and E.coli strain ATCC 8739 (MIC=30 ug). Antibacterial activity is
CC       decreased by acetylation. {ECO:0000269|PubMed:21882228}.
CC   -!- MASS SPECTROMETRY: Mass=1050.89; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:21882228};
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DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; DNA-binding.
FT   PEPTIDE         1..9
FT                   /note="Anticancerous peptide 1"
FT                   /evidence="ECO:0000269|PubMed:21882228"
FT                   /id="PRO_0000415956"
SQ   SEQUENCE   9 AA;  1050 MW;  39CD7AAAA9C72323 CRC64;
     AWKLFDDGV