ID   COBU_SINSX              Reviewed;         338 AA.
AC   P29935;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase;
DE            Short=NN:DBI PRT;
DE            EC=2.4.2.21;
DE   AltName: Full=N(1)-alpha-phosphoribosyltransferase;
GN   Name=cobU;
OS   Sinorhizobium sp.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=42445;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-16.
RC   STRAIN=SC510;
RX   PubMed=1917841; DOI=10.1128/jb.173.19.6066-6073.1991;
RA   Cameron B., Blanche F., Rouyez M.-C., Bisch D., Famechon A., Couder M.,
RA   Cauchois L., Thibaut D., Debussche L., Crouzet J.;
RT   "Genetic analysis, nucleotide sequence, and products of two Pseudomonas
RT   denitrificans cob genes encoding nicotinate-nucleotide:
RT   dimethylbenzimidazole phosphoribosyltransferase and cobalamin (5'-
RT   phosphate) synthase.";
RL   J. Bacteriol. 173:6066-6073(1991).
CC   -!- FUNCTION: Catalyzes the synthesis of alpha-ribazole-5'-phosphate from
CC       nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dimethylbenzimidazole + nicotinate beta-D-ribonucleotide =
CC         alpha-ribazole 5'-phosphate + H(+) + nicotinate;
CC         Xref=Rhea:RHEA:11196, ChEBI:CHEBI:15378, ChEBI:CHEBI:15890,
CC         ChEBI:CHEBI:32544, ChEBI:CHEBI:57502, ChEBI:CHEBI:57918; EC=2.4.2.21;
CC   -!- PATHWAY: Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-
CC       ribazole from 5,6-dimethylbenzimidazole: step 1/2.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the CobT family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to originate from Pseudomonas
CC       denitrificans, but similarity searches show that the sequence is much
CC       closer to Sinorhizobium. The entry's taxonomy has been changed.
CC       {ECO:0000305}.
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DR   EMBL; M62868; AAA25788.1; -; Genomic_DNA.
DR   AlphaFoldDB; P29935; -.
DR   SMR; P29935; -.
DR   KEGG; ag:AAA25788; -.
DR   BioCyc; MetaCyc:MON-13241; -.
DR   SABIO-RK; P29935; -.
DR   UniPathway; UPA00061; UER00516.
DR   GO; GO:0008939; F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd02439; DMB-PRT_CobT; 1.
DR   Gene3D; 1.10.1610.10; -; 1.
DR   Gene3D; 3.40.50.10210; -; 1.
DR   HAMAP; MF_00230; CobT; 1.
DR   InterPro; IPR003200; Nict_dMeBzImd_PRibTrfase.
DR   InterPro; IPR017846; Nict_dMeBzImd_PRibTrfase_bact.
DR   InterPro; IPR023195; Nict_dMeBzImd_PRibTrfase_N.
DR   InterPro; IPR036087; Nict_dMeBzImd_PRibTrfase_sf.
DR   Pfam; PF02277; DBI_PRT; 1.
DR   SUPFAM; SSF52733; SSF52733; 1.
DR   TIGRFAMs; TIGR03160; cobT_DBIPRT; 1.
PE   1: Evidence at protein level;
KW   Cobalamin biosynthesis; Direct protein sequencing; Glycosyltransferase;
KW   Transferase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:1917841"
FT   CHAIN           2..338
FT                   /note="Nicotinate-nucleotide--dimethylbenzimidazole
FT                   phosphoribosyltransferase"
FT                   /id="PRO_0000167061"
FT   ACT_SITE        305
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   338 AA;  34682 MW;  B7E7B51CCA471861 CRC64;
     MSASGLPFDD FRELLRNLPG PDAAALVAAR ERDAQLTKPP GALGRLEEIA FWLAAWTGKA
     PVVNRPLVAI FAGNHGVTRQ GVTPFPSSVT AQMVENFAAG GAAINQICVS HDLGLKVFDL
     ALEYPTGDIT EEAALSERDC AATMAFGMEA IAGGTDLLCI GEMGIGNTTI AAAINLGLYG
     GTAEEWVGPG TGSEGEVLKR KIAAVEKAVA LHRDHLSDPL ELMRRLGGRE IAAMAGAILA
     ARVQKVPVII DGYVATAAAS ILKAANPSAL DHCLIGHVSG EPGHLRAIEK LGKTPLLALG
     MRLGEGTGAA LAAGIVKAAA ACHSGMATFA QAGVSNKE