ID   COBV_SINSX              Reviewed;         262 AA.
AC   P29936;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   10-OCT-2003, sequence version 2.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Adenosylcobinamide-GDP ribazoletransferase;
DE            EC=2.7.8.26;
DE   AltName: Full=Cobalamin synthase;
DE   AltName: Full=Cobalamin-5'-phosphate synthase;
GN   Name=cobV;
OS   Sinorhizobium sp.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=42445;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR,
RP   AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=SC510;
RX   PubMed=1917841; DOI=10.1128/jb.173.19.6066-6073.1991;
RA   Cameron B., Blanche F., Rouyez M.-C., Bisch D., Famechon A., Couder M.,
RA   Cauchois L., Thibaut D., Debussche L., Crouzet J.;
RT   "Genetic analysis, nucleotide sequence, and products of two Pseudomonas
RT   denitrificans cob genes encoding nicotinate-nucleotide:
RT   dimethylbenzimidazole phosphoribosyltransferase and cobalamin (5'-
RT   phosphate) synthase.";
RL   J. Bacteriol. 173:6066-6073(1991).
CC   -!- FUNCTION: Joins adenosylcobinamide-GDP and alpha-ribazole to generate
CC       adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin
CC       5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-
CC       phosphate. {ECO:0000269|PubMed:1917841}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosylcob(III)inamide-GDP + alpha-ribazole =
CC         adenosylcob(III)alamin + GMP + H(+); Xref=Rhea:RHEA:16049,
CC         ChEBI:CHEBI:10329, ChEBI:CHEBI:15378, ChEBI:CHEBI:18408,
CC         ChEBI:CHEBI:58115, ChEBI:CHEBI:60487; EC=2.7.8.26;
CC         Evidence={ECO:0000269|PubMed:1917841};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosylcob(III)inamide-GDP + alpha-ribazole 5'-phosphate =
CC         adenosylcob(III)alamin 5'-phosphate + GMP + H(+);
CC         Xref=Rhea:RHEA:23560, ChEBI:CHEBI:15378, ChEBI:CHEBI:57918,
CC         ChEBI:CHEBI:58115, ChEBI:CHEBI:60487, ChEBI:CHEBI:60493; EC=2.7.8.26;
CC         Evidence={ECO:0000269|PubMed:1917841};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000269|PubMed:1917841};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=2.7 uM for alpha-ribazole 5'-phosphate
CC         {ECO:0000269|PubMed:1917841};
CC         KM=0.9 uM for adenosylcobinamide-GDP {ECO:0000269|PubMed:1917841};
CC         KM=7.8 uM for alpha-ribazole {ECO:0000269|PubMed:1917841};
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.
CC   -!- SUBUNIT: Associated with a large complex of proteins.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CobS family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to originate from Pseudomonas
CC       denitrificans, but similarity searches show that the sequence is much
CC       closer to Sinorhizobium. The entry's taxonomy has been changed.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA25787.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; M62868; AAA25787.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; P29936; -.
DR   BioCyc; MetaCyc:MON-144; -.
DR   SABIO-RK; P29936; -.
DR   UniPathway; UPA00148; UER00238.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051073; F:adenosylcobinamide-GDP ribazoletransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008818; F:cobalamin 5'-phosphate synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00719; CobS; 1.
DR   InterPro; IPR003805; CobS.
DR   PANTHER; PTHR34148; PTHR34148; 1.
DR   Pfam; PF02654; CobS; 1.
DR   TIGRFAMs; TIGR00317; cobS; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Cobalamin biosynthesis; Magnesium;
KW   Membrane; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..262
FT                   /note="Adenosylcobinamide-GDP ribazoletransferase"
FT                   /id="PRO_0000146888"
FT   TRANSMEM        41..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        115..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   262 AA;  26842 MW;  2E2E833C94518D5F CRC64;
     MGFVGDFCDD VARSIGFLSR IPMPARHFEG YDGRLSRAVR AFPFAGLAIA LPSAAVAMAL
     MALQVSSLFA AFVVVAIQAL VTGALHEDGL GDTADGFGGG RDREAALAIM KDSRIGTYAA
     VALILSFGLR VSAFASILPL FSPLGAAMAI LGAACLSRAA MVWHWSSLPP ARSSGVAASA
     GEPEPAATRF ALAFGLLVAM LLFYLAQVPA LGVIAALVAF LATVKGFARL AMRKIGGQTG
     DTIGATQQLT EIAVLGALAL TV