COCH_MOUSE
ID COCH_MOUSE Reviewed; 552 AA.
AC Q62507; Q3TAF5; Q9QWK6;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Cochlin;
DE AltName: Full=COCH-5B2;
DE Flags: Precursor;
GN Name=Coch;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Cochlea;
RX PubMed=9441737; DOI=10.1006/geno.1997.5067;
RA Robertson N.G., Skvorak A.B., Yin Y., Weremowicz S., Johnson K.R.,
RA Kovatch K.A., Battey J.F., Bieber F.R., Morton C.C.;
RT "Mapping and characterization of a novel cochlear gene in human and in
RT mouse: a positional candidate gene for a deafness disorder, DFNA9.";
RL Genomics 46:345-354(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Skin;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 324-417.
RC TISSUE=Cochlea;
RX PubMed=9119401; DOI=10.1006/geno.1996.4526;
RA Crozet F., El-Amraoui A., Blanchard S., Lenoir M., Ripoll C., Vago P.,
RA Hamel C., Fizames C., Levi-Acobas F., Depetris D., Mattei M.-G., Weil D.,
RA Pujol R., Petit C.;
RT "Cloning of the genes encoding two murine and human cochlear unconventional
RT type I myosins.";
RL Genomics 40:332-341(1997).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a role in the control of cell shape and motility in the
CC trabecular meshwork. {ECO:0000250}.
CC -!- SUBUNIT: Monomer. May form homodimer. Interacts with type II collagen.
CC Interacts with SLC44A2. Interacts with ANXA2.
CC {ECO:0000250|UniProtKB:O43405}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space
CC {ECO:0000250|UniProtKB:O43405}.
CC -!- TISSUE SPECIFICITY: Expressed in inner ear structures.
CC -!- PTM: N-glycosylated. {ECO:0000250|UniProtKB:O43405}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=The Japanese Horseshoe Crab
CC and Deafness - Issue 4 of November 2000;
CC URL="https://web.expasy.org/spotlight/back_issues/004";
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DR EMBL; AF006741; AAC39949.1; -; mRNA.
DR EMBL; AK028690; BAC26067.1; -; mRNA.
DR EMBL; AK137336; BAE23310.1; -; mRNA.
DR EMBL; AK171875; BAE42713.1; -; mRNA.
DR EMBL; BC045137; AAH45137.1; -; mRNA.
DR EMBL; Z78163; CAB01565.1; -; mRNA.
DR CCDS; CCDS25901.1; -.
DR RefSeq; NP_001185764.1; NM_001198835.1.
DR RefSeq; NP_031754.1; NM_007728.5.
DR AlphaFoldDB; Q62507; -.
DR SMR; Q62507; -.
DR BioGRID; 198801; 1.
DR STRING; 10090.ENSMUSP00000128127; -.
DR GlyGen; Q62507; 2 sites.
DR iPTMnet; Q62507; -.
DR PhosphoSitePlus; Q62507; -.
DR MaxQB; Q62507; -.
DR PaxDb; Q62507; -.
DR PRIDE; Q62507; -.
DR ProteomicsDB; 283785; -.
DR Antibodypedia; 22996; 213 antibodies from 26 providers.
DR DNASU; 12810; -.
DR Ensembl; ENSMUST00000085412; ENSMUSP00000082533; ENSMUSG00000020953.
DR Ensembl; ENSMUST00000164782; ENSMUSP00000128127; ENSMUSG00000020953.
DR GeneID; 12810; -.
DR KEGG; mmu:12810; -.
DR UCSC; uc007nmr.2; mouse.
DR CTD; 1690; -.
DR MGI; MGI:1278313; Coch.
DR VEuPathDB; HostDB:ENSMUSG00000020953; -.
DR eggNOG; KOG1216; Eukaryota.
DR GeneTree; ENSGT00940000159386; -.
DR HOGENOM; CLU_019512_1_0_1; -.
DR InParanoid; Q62507; -.
DR OMA; CPANCPL; -.
DR OrthoDB; 200139at2759; -.
DR PhylomeDB; Q62507; -.
DR TreeFam; TF318242; -.
DR BioGRID-ORCS; 12810; 5 hits in 73 CRISPR screens.
DR ChiTaRS; Coch; mouse.
DR PRO; PR:Q62507; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q62507; protein.
DR Bgee; ENSMUSG00000020953; Expressed in utricle of membranous labyrinth and 128 other tissues.
DR Genevisible; Q62507; MM.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; ISO:MGI.
DR GO; GO:0031012; C:extracellular matrix; IDA:MGI.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0005518; F:collagen binding; ISS:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; IEA:InterPro.
DR GO; GO:0045089; P:positive regulation of innate immune response; IEA:InterPro.
DR GO; GO:0008360; P:regulation of cell shape; ISS:UniProtKB.
DR GO; GO:0007605; P:sensory perception of sound; IMP:MGI.
DR Gene3D; 2.170.130.20; -; 1.
DR Gene3D; 3.40.50.410; -; 2.
DR InterPro; IPR030743; Cochlin.
DR InterPro; IPR004043; LCCL.
DR InterPro; IPR036609; LCCL_sf.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR24020:SF36; PTHR24020:SF36; 2.
DR Pfam; PF03815; LCCL; 1.
DR Pfam; PF00092; VWA; 2.
DR SMART; SM00603; LCCL; 1.
DR SMART; SM00327; VWA; 2.
DR SUPFAM; SSF53300; SSF53300; 2.
DR SUPFAM; SSF69848; SSF69848; 1.
DR PROSITE; PS50820; LCCL; 1.
DR PROSITE; PS50234; VWFA; 2.
PE 1: Evidence at protein level;
KW Disulfide bond; Glycoprotein; Hearing; Reference proteome; Repeat;
KW Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..552
FT /note="Cochlin"
FT /id="PRO_0000020969"
FT DOMAIN 30..123
FT /note="LCCL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DOMAIN 167..352
FT /note="VWFA 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT DOMAIN 369..539
FT /note="VWFA 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT REGION 129..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 102
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 223
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 36..52
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DISULFID 56..76
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
SQ SEQUENCE 552 AA; 59950 MW; AD0BBB48D20055E3 CRC64;
MPSSRIPALC LGAWLLLLLL PRFARAEGAV PIPVTCFTRG LDIRKEKADV LCPGGCSLEE
FSVFGNIVYA SVSSICGAAV HRGVIGTSGG PVRVYSLPGR ENYSSVDANG IQSQMLSRWS
ASFAVTKGKS STQEATGRAV STAHPPSGKR LKKTPEKKTG NKDCKADIAF LIDGSFNIGQ
RRFNLQKNFV GKVALMLGIG TEGPHVGLVQ ASEHPKIEFY LKNFTSAKDV LFAIKEVGFR
GGNSNTGKAL KHTAQKFFTA DTGVRKGIPK VVVVFIDGWP SDDIEEAGIV AREFGVNVFI
VSVAKPIPEE LGMVQDVAFV DKAVCRNNGF FSYHMPNWFG TTKYVKPLVQ KLCTHEQMMC
SKTCYNSVNI AFLIDGSSSV GDSNFRLMLE FVSNIAKTFE ISDIGAKIAA VQFTYDQRTE
FSFTDYNTKE NVLAVLANIR YMSGGTATGD AIAFTVRNVF GPIRDSPNKN FLVIVTDGQS
YDDVRGPAAA AHDAGITIFS VGVAWAPLDD LRDMASKPKE SHAFFTREFT GLEPIVSDVI
RGICRDFLES QQ
