ID   ACP2_HORVU              Reviewed;         129 AA.
AC   P08817;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 2.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Acyl carrier protein 2, chloroplastic;
DE   AltName: Full=Acyl carrier protein II;
DE            Short=ACP II;
DE   Flags: Precursor;
GN   Name=ACL1.2; Synonyms=ACL2;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Svalofs Bonus;
RX   PubMed=16668413; DOI=10.1104/pp.97.1.472;
RA   Hansen L., Kauppinen S.;
RT   "Barley acyl carrier protein II: nucleotide sequence of cDNA clones and
RT   chromosomal location of the Acl2 gene.";
RL   Plant Physiol. 97:472-474(1991).
RN   [2]
RP   PROTEIN SEQUENCE OF 50-73.
RC   STRAIN=cv. Svalofs Bonus;
RA   Hoej P.B., Svendsen I.;
RT   "Barley chloroplasts contain two acyl carrier proteins coded for by
RT   different genes.";
RL   Carlsberg Res. Commun. 49:483-492(1984).
CC   -!- FUNCTION: Carrier of the growing fatty acid chain in fatty acid
CC       biosynthesis.
CC   -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- PTM: 4'-phosphopantetheine is transferred from CoA to a specific serine
CC       of apo-ACP by acpS. This modification is essential for activity because
CC       fatty acids are bound in thioester linkage to the sulfhydryl of the
CC       prosthetic group (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the acyl carrier protein (ACP) family.
CC       {ECO:0000305}.
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DR   EMBL; M63799; AAA32921.1; -; mRNA.
DR   PIR; T10175; T10175.
DR   AlphaFoldDB; P08817; -.
DR   SMR; P08817; -.
DR   EnsemblPlants; HORVU.MOREX.r2.5HG0415590.1; HORVU.MOREX.r2.5HG0415590.1; HORVU.MOREX.r2.5HG0415590.
DR   EnsemblPlants; HORVU.MOREX.r2.5HG0415590.1.mrna1; HORVU.MOREX.r2.5HG0415590.1.mrna1; HORVU.MOREX.r2.5HG0415590.1.
DR   Gramene; HORVU.MOREX.r2.5HG0415590.1; HORVU.MOREX.r2.5HG0415590.1; HORVU.MOREX.r2.5HG0415590.
DR   Gramene; HORVU.MOREX.r2.5HG0415590.1.mrna1; HORVU.MOREX.r2.5HG0415590.1.mrna1; HORVU.MOREX.r2.5HG0415590.1.
DR   UniPathway; UPA00094; -.
DR   ExpressionAtlas; P08817; baseline and differential.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000036; F:acyl carrier activity; IEA:InterPro.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   Gene3D; 1.10.1200.10; -; 1.
DR   HAMAP; MF_01217; Acyl_carrier; 1.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR044813; ACP_chloroplastic.
DR   InterPro; IPR003231; Acyl_carrier.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   InterPro; IPR006162; Ppantetheine_attach_site.
DR   PANTHER; PTHR46153; PTHR46153; 1.
DR   Pfam; PF00550; PP-binding; 1.
DR   SMART; SM00823; PKS_PP; 1.
DR   SUPFAM; SSF47336; SSF47336; 1.
DR   TIGRFAMs; TIGR00517; acyl_carrier; 1.
DR   PROSITE; PS50075; CARRIER; 1.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Direct protein sequencing; Fatty acid biosynthesis;
KW   Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Phosphopantetheine; Phosphoprotein; Plastid; Transit peptide.
FT   TRANSIT         1..49
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000269|Ref.2"
FT   CHAIN           50..129
FT                   /note="Acyl carrier protein 2, chloroplastic"
FT                   /id="PRO_0000000582"
FT   DOMAIN          52..127
FT                   /note="Carrier"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT   MOD_RES         87
FT                   /note="O-(pantetheine 4'-phosphoryl)serine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT   CONFLICT        72
FT                   /note="T -> D (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   129 AA;  13464 MW;  32B3E5A8D3AB1D22 CRC64;
     MASAAASAVS FARPVKAICV NSVSFSALRK DNVSFRLQPV PQRFSVCCAA KKETVEKVCD
     IVKSQLALSD DTEVSGSSTF ADLGADSLDT VEIVMGLEEA FGISVEESSA QTIATVEDAA
     NLIDSLVGK