ID   CODY_LACLA              Reviewed;         262 AA.
AC   Q9CJ45;
DT   28-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=Transcriptional regulator;
GN   Name=codY; OrderedLocusNames=LL0161; ORFNames=L0243;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
RN   [2]
RP   FUNCTION.
RX   PubMed=11401725; DOI=10.1046/j.1365-2958.2001.02470.x;
RA   Guedon E., Serror P., Ehrlich S.D., Renault P., Delorme C.;
RT   "Pleiotropic transcriptional repressor CodY senses the intracellular pool
RT   of branched-chain amino acids in Lactococcus lactis.";
RL   Mol. Microbiol. 40:1227-1239(2001).
CC   -!- FUNCTION: DNA-binding protein that represses the expression of many
CC       genes essential for supplying Lactococcus lactis with amino acids
CC       during growth in milk. Seem to act on the major components of the
CC       proteolytic system, including the cell wall proteinase (PrtP), the
CC       oligopeptide transport system (Opp) and at least four intracellular
CC       peptidases (PepO1, PepN, PepC, PepDA2). Seem to be regulated via the
CC       dipeptide content in branched-chain amino acids (BCAA; isoleucine,
CC       leucine or valine). The BCAA may act directly or indirectly through
CC       GTP. May be a GTP-binding protein that senses the intracellular GTP
CC       concentration as an indicator of nutritional limitations. At low GTP
CC       concentration it no longer binds GTP and stop to act as a
CC       transcriptional repressor (By similarity). {ECO:0000250,
CC       ECO:0000269|PubMed:11401725}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the CodY family. {ECO:0000305}.
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DR   EMBL; AE005176; AAK04259.1; -; Genomic_DNA.
DR   PIR; A86645; A86645.
DR   RefSeq; NP_266317.1; NC_002662.1.
DR   RefSeq; WP_003129722.1; NC_002662.1.
DR   AlphaFoldDB; Q9CJ45; -.
DR   SMR; Q9CJ45; -.
DR   STRING; 272623.L0243; -.
DR   PaxDb; Q9CJ45; -.
DR   EnsemblBacteria; AAK04259; AAK04259; L0243.
DR   GeneID; 60355103; -.
DR   GeneID; 66441125; -.
DR   KEGG; lla:L0243; -.
DR   PATRIC; fig|272623.7.peg.178; -.
DR   eggNOG; COG4465; Bacteria.
DR   HOGENOM; CLU_089581_0_0_9; -.
DR   OMA; MPFVNVD; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.30.450.40; -; 1.
DR   HAMAP; MF_00621; HTH_type_CodY; 1.
DR   InterPro; IPR014154; CodY.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR010312; GTP_sensing_CodY_N.
DR   InterPro; IPR013198; GTP_sensing_HTH_CodY_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR40062; PTHR40062; 1.
DR   Pfam; PF06018; CodY; 1.
DR   Pfam; PF08222; HTH_CodY; 1.
DR   PIRSF; PIRSF011572; GTP_sensing_CodY; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR02787; codY_Gpos; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; GTP-binding; Nucleotide-binding;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..262
FT                   /note="Transcriptional regulator"
FT                   /id="PRO_0000213226"
FT   DNA_BIND        206..225
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   262 AA;  29128 MW;  6BD690023E38C129 CRC64;
     MATLLEKTRK ITAILQDGVT DLQQELPYNS MTERLANVID CNACVINTKG ELLGYSLPYN
     TNNDRVDQFF YDRKLPDEYV RAAVRIYDTM ANVPVDRPLA IFPEESLGDF PKGVTTLAPI
     YGSGMRLGTF IMWREDGEFT DDDLVLVELA TTVIGVQLSN LKLEQMEENI RKDTMATMAV
     NTLSYSEMKA VKAIIEELDG EEGHVIASVI ADKIGITRSV IVNALRKLES AGVIESRSLG
     MKGTYLKVLN TGLFDKLAGR NF