COE2_HUMAN
ID COE2_HUMAN Reviewed; 575 AA.
AC Q9HAK2; A0PJM4; A6NMF7; F5H645; Q66VZ3; Q6DK36; Q6IS86; Q6ISA4;
DT 28-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 12-DEC-2006, sequence version 4.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Transcription factor COE2;
DE AltName: Full=Early B-cell factor 2;
DE Short=EBF-2;
GN Name=EBF2; Synonyms=COE2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Li H., Shen C., Zhou G., Ke R., Zhong G., Lin L., Yang S.;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 233-575 (ISOFORM 1).
RC TISSUE=Embryo;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 240-575 (ISOFORM 1).
RC TISSUE=Brain, and Fetal brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INTERACTION WITH SIX1.
RX PubMed=27923061; DOI=10.1371/journal.pgen.1006474;
RA Brunmeir R., Wu J., Peng X., Kim S.Y., Julien S.G., Zhang Q., Xie W.,
RA Xu F.;
RT "Comparative Transcriptomic and Epigenomic Analyses Reveal New Regulators
RT of Murine Brown Adipogenesis.";
RL PLoS Genet. 12:E1006474-E1006474(2016).
CC -!- FUNCTION: Transcription factor that, in osteoblasts, activates the
CC decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast
CC differentiation. Acts in synergy with the Wnt-responsive LEF1/CTNNB1
CC pathway. Recognizes variations of the palindromic sequence 5'-
CC ATTCCCNNGGGAATT-3' (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Forms either a homodimer or a heterodimer with a related
CC family member (By similarity). Interacts with SIX1 (PubMed:27923061).
CC {ECO:0000250, ECO:0000250|UniProtKB:O08792,
CC ECO:0000269|PubMed:27923061}.
CC -!- INTERACTION:
CC Q9HAK2; Q9H4W6-2: EBF3; NbExp=3; IntAct=EBI-12267154, EBI-17233744;
CC Q9HAK2; Q13077: TRAF1; NbExp=3; IntAct=EBI-12267154, EBI-359224;
CC Q9HAK2; Q12933: TRAF2; NbExp=3; IntAct=EBI-12267154, EBI-355744;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9HAK2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9HAK2-2; Sequence=VSP_055530, VSP_055531;
CC -!- SIMILARITY: Belongs to the COE family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB13843.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AY700779; AAU10086.1; -; mRNA.
DR EMBL; AC023566; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC090103; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK021562; BAB13843.1; ALT_INIT; mRNA.
DR EMBL; BC069665; AAH69665.1; -; mRNA.
DR EMBL; BC069726; AAH69726.1; -; mRNA.
DR EMBL; BC069747; AAH69747.1; -; mRNA.
DR EMBL; BC069768; AAH69768.1; -; mRNA.
DR EMBL; BC074794; AAH74794.2; -; mRNA.
DR EMBL; BC113478; AAI13479.1; -; mRNA.
DR EMBL; BC113504; AAI13505.1; -; mRNA.
DR CCDS; CCDS43726.1; -. [Q9HAK2-1]
DR RefSeq; NP_073150.2; NM_022659.3. [Q9HAK2-1]
DR AlphaFoldDB; Q9HAK2; -.
DR SMR; Q9HAK2; -.
DR BioGRID; 122221; 65.
DR IntAct; Q9HAK2; 4.
DR STRING; 9606.ENSP00000430241; -.
DR iPTMnet; Q9HAK2; -.
DR PhosphoSitePlus; Q9HAK2; -.
DR SwissPalm; Q9HAK2; -.
DR BioMuta; EBF2; -.
DR DMDM; 119370337; -.
DR EPD; Q9HAK2; -.
DR MassIVE; Q9HAK2; -.
DR MaxQB; Q9HAK2; -.
DR PaxDb; Q9HAK2; -.
DR PeptideAtlas; Q9HAK2; -.
DR PRIDE; Q9HAK2; -.
DR ProteomicsDB; 27076; -.
DR ProteomicsDB; 81409; -. [Q9HAK2-1]
DR Antibodypedia; 22905; 110 antibodies from 21 providers.
DR DNASU; 64641; -.
DR Ensembl; ENST00000520164.6; ENSP00000430241.1; ENSG00000221818.9. [Q9HAK2-1]
DR Ensembl; ENST00000535548.1; ENSP00000437909.1; ENSG00000221818.9. [Q9HAK2-2]
DR GeneID; 64641; -.
DR KEGG; hsa:64641; -.
DR MANE-Select; ENST00000520164.6; ENSP00000430241.1; NM_022659.4; NP_073150.2.
DR UCSC; uc003xes.3; human. [Q9HAK2-1]
DR CTD; 64641; -.
DR DisGeNET; 64641; -.
DR GeneCards; EBF2; -.
DR HGNC; HGNC:19090; EBF2.
DR HPA; ENSG00000221818; Tissue enhanced (adipose).
DR MIM; 609934; gene.
DR neXtProt; NX_Q9HAK2; -.
DR OpenTargets; ENSG00000221818; -.
DR PharmGKB; PA38794; -.
DR VEuPathDB; HostDB:ENSG00000221818; -.
DR eggNOG; KOG3836; Eukaryota.
DR GeneTree; ENSGT00950000182859; -.
DR HOGENOM; CLU_016320_3_1_1; -.
DR InParanoid; Q9HAK2; -.
DR OMA; YGTSGGM; -.
DR PhylomeDB; Q9HAK2; -.
DR TreeFam; TF313391; -.
DR PathwayCommons; Q9HAK2; -.
DR SignaLink; Q9HAK2; -.
DR BioGRID-ORCS; 64641; 17 hits in 1090 CRISPR screens.
DR ChiTaRS; EBF2; human.
DR GenomeRNAi; 64641; -.
DR Pharos; Q9HAK2; Tbio.
DR PRO; PR:Q9HAK2; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q9HAK2; protein.
DR Bgee; ENSG00000221818; Expressed in dorsal root ganglion and 126 other tissues.
DR ExpressionAtlas; Q9HAK2; baseline and differential.
DR Genevisible; Q9HAK2; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003682; F:chromatin binding; IEA:Ensembl.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0060612; P:adipose tissue development; IEA:Ensembl.
DR GO; GO:0050873; P:brown fat cell differentiation; IEA:Ensembl.
DR GO; GO:0001709; P:cell fate determination; IEA:Ensembl.
DR GO; GO:0035563; P:positive regulation of chromatin binding; IEA:Ensembl.
DR GO; GO:0120162; P:positive regulation of cold-induced thermogenesis; ISS:YuBioLab.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd11606; COE_DBD; 1.
DR CDD; cd01175; IPT_COE; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 2.60.40.3180; -; 1.
DR InterPro; IPR032200; COE_DBD.
DR InterPro; IPR038173; COE_DBD_sf.
DR InterPro; IPR032201; COE_HLH.
DR InterPro; IPR038006; COE_IPT.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR002909; IPT_dom.
DR InterPro; IPR003523; Transcription_factor_COE.
DR InterPro; IPR018350; Transcription_factor_COE_CS.
DR PANTHER; PTHR10747; PTHR10747; 1.
DR Pfam; PF16422; COE1_DBD; 1.
DR Pfam; PF16423; COE1_HLH; 1.
DR Pfam; PF01833; TIG; 1.
DR SMART; SM00429; IPT; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
DR PROSITE; PS01345; COE; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Developmental protein; DNA-binding;
KW Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..575
FT /note="Transcription factor COE2"
FT /id="PRO_0000107828"
FT DOMAIN 253..336
FT /note="IPT/TIG"
FT ZN_FING 150..169
FT /note="C5-type"
FT /evidence="ECO:0000255"
FT REGION 62..65
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT REGION 196..203
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT REGION 235..238
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT REGION 441..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 162
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT SITE 171
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..269
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_055530"
FT VAR_SEQ 510..575
FT /note="IMSSSPTVGSSSTSSILPFSSSVFPAVKQKSAFAPVIRPQGSPSPACSSGNG
FT NGFRAMTGLVVPPM -> TGLWLTWLLKAFWGKKRIETIPFL (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_055531"
FT VARIANT 559
FT /note="G -> S (in dbSNP:rs17054477)"
FT /id="VAR_048754"
FT CONFLICT 483
FT /note="S -> G (in Ref. 1; AAU10086)"
FT /evidence="ECO:0000305"
FT CONFLICT 513
FT /note="S -> L (in Ref. 3; BAB13843)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 575 AA; 62650 MW; 05FCDBC9CB81EC89 CRC64;
MFGIQDTLGR GPTLKEKSLG AEMDSVRSWV RNVGVVDANV AAQSGVALSR AHFEKQPPSN
LRKSNFFHFV LALYDRQGQP VEIERTAFVD FVENDKEQGN EKTNNGTHYK LQLLYSNGVR
TEQDLYVRLI DSVTKQPIAY EGQNKNPEMC RVLLTHEVMC SRCCEKKSCG NRNETPSDPV
IIDRFFLKFF LKCNQNCLKT AGNPRDMRRF QVVLSTTVNV DGHVLAVSDN MFVHNNSKHG
RRARRLDPSE ATPCIKAISP SEGWTTGGAM VIIIGDNFFD GLQVVFGTML VWSELITPHA
IRVQTPPRHI PGVVEVTLSY KSKQFCKGAP GRFIYTALNE PTIDYGFQRL QKVIPRHPGD
PERLAKEMLL KRAADLVEAL YGTPHNNQDI ILKRAADIAE ALYSVPRNPS QLPALSSSPA
HSGMMGINSY GSQLGVSISE STQGNNQGYI RNTSSISPRG YSSSSTPQQS NYSTSSNSMN
GYSNVPMANL GVPGSPGFLN GSPTGSPYGI MSSSPTVGSS STSSILPFSS SVFPAVKQKS
AFAPVIRPQG SPSPACSSGN GNGFRAMTGL VVPPM
