COE4_HUMAN
ID COE4_HUMAN Reviewed; 602 AA.
AC Q9BQW3; Q1MTP7; Q5JY53; Q9NUB6; Q9P2A6;
DT 10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Transcription factor COE4;
DE AltName: Full=Early B-cell factor 4;
DE Short=EBF-4;
DE AltName: Full=Olf-1/EBF-like 4;
DE Short=O/E-4;
DE Short=OE-4;
GN Name=EBF4; Synonyms=COE4, KIAA1442;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11780052; DOI=10.1038/414865a;
RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 20.";
RL Nature 414:865-871(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-602 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=10718198; DOI=10.1093/dnares/7.1.65;
RA Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XVI. The
RT complete sequences of 150 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 7:65-73(2000).
CC -!- FUNCTION: Transcriptional factor which recognizes variations of the
CC palindromic sequence 5'-ATTCCCNNGGGAATT-3'. {ECO:0000250}.
CC -!- SUBUNIT: Forms either a homodimer or a heterodimer with a related
CC family member. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=2;
CC IsoId=Q9BQW3-2; Sequence=Displayed;
CC Name=1;
CC IsoId=Q9BQW3-1; Sequence=VSP_026469, VSP_026470;
CC -!- SIMILARITY: Belongs to the COE family. {ECO:0000305}.
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DR EMBL; AL035460; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AB037863; BAA92680.1; -; mRNA.
DR RefSeq; NP_001103984.1; NM_001110514.1.
DR AlphaFoldDB; Q9BQW3; -.
DR SMR; Q9BQW3; -.
DR BioGRID; 121644; 4.
DR IntAct; Q9BQW3; 1.
DR STRING; 9606.ENSP00000370022; -.
DR GlyGen; Q9BQW3; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9BQW3; -.
DR PhosphoSitePlus; Q9BQW3; -.
DR BioMuta; EBF4; -.
DR DMDM; 152031576; -.
DR jPOST; Q9BQW3; -.
DR MassIVE; Q9BQW3; -.
DR MaxQB; Q9BQW3; -.
DR PaxDb; Q9BQW3; -.
DR PeptideAtlas; Q9BQW3; -.
DR PRIDE; Q9BQW3; -.
DR ProteomicsDB; 78725; -. [Q9BQW3-2]
DR ProteomicsDB; 78726; -. [Q9BQW3-1]
DR TopDownProteomics; Q9BQW3-1; -. [Q9BQW3-1]
DR Antibodypedia; 23277; 91 antibodies from 17 providers.
DR DNASU; 57593; -.
DR Ensembl; ENST00000609451.5; ENSP00000477023.1; ENSG00000088881.21. [Q9BQW3-2]
DR GeneID; 57593; -.
DR KEGG; hsa:57593; -.
DR MANE-Select; ENST00000609451.6; ENSP00000477023.1; NM_001395167.1; NP_001382096.1.
DR UCSC; uc061vby.1; human. [Q9BQW3-2]
DR CTD; 57593; -.
DR GeneCards; EBF4; -.
DR HGNC; HGNC:29278; EBF4.
DR HPA; ENSG00000088881; Low tissue specificity.
DR MIM; 609935; gene.
DR neXtProt; NX_Q9BQW3; -.
DR OpenTargets; ENSG00000088881; -.
DR PharmGKB; PA162384262; -.
DR VEuPathDB; HostDB:ENSG00000088881; -.
DR eggNOG; KOG3836; Eukaryota.
DR GeneTree; ENSGT00950000182859; -.
DR InParanoid; Q9BQW3; -.
DR OMA; QPGYARS; -.
DR OrthoDB; 817293at2759; -.
DR PhylomeDB; Q9BQW3; -.
DR PathwayCommons; Q9BQW3; -.
DR SignaLink; Q9BQW3; -.
DR BioGRID-ORCS; 57593; 20 hits in 1100 CRISPR screens.
DR ChiTaRS; EBF4; human.
DR GenomeRNAi; 57593; -.
DR Pharos; Q9BQW3; Tbio.
DR PRO; PR:Q9BQW3; -.
DR Proteomes; UP000005640; Chromosome 20.
DR RNAct; Q9BQW3; protein.
DR Bgee; ENSG00000088881; Expressed in right lobe of thyroid gland and 142 other tissues.
DR ExpressionAtlas; Q9BQW3; baseline and differential.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd11606; COE_DBD; 1.
DR CDD; cd01175; IPT_COE; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 2.60.40.3180; -; 1.
DR InterPro; IPR032200; COE_DBD.
DR InterPro; IPR038173; COE_DBD_sf.
DR InterPro; IPR032201; COE_HLH.
DR InterPro; IPR038006; COE_IPT.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR002909; IPT_dom.
DR InterPro; IPR003523; Transcription_factor_COE.
DR InterPro; IPR018350; Transcription_factor_COE_CS.
DR PANTHER; PTHR10747; PTHR10747; 1.
DR Pfam; PF16422; COE1_DBD; 1.
DR Pfam; PF16423; COE1_HLH; 1.
DR Pfam; PF01833; TIG; 1.
DR SMART; SM00429; IPT; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
DR PROSITE; PS01345; COE; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Developmental protein; DNA-binding;
KW Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..602
FT /note="Transcription factor COE4"
FT /id="PRO_0000107835"
FT DOMAIN 256..338
FT /note="IPT/TIG"
FT ZN_FING 152..171
FT /note="C5-type"
FT /evidence="ECO:0000255"
FT REGION 64..67
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT REGION 198..205
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT REGION 237..240
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT REGION 448..476
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 558..602
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 460..474
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 164
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT SITE 173
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..5
FT /note="MFPAQ -> MARARGQAPGSGGRRRPRRGGAGTGPRGESAGLLLLLLQVLPP
FT GATAGPGRRGTRGAGGGGVCWPGAAPAFPRDARLLLLLPPLPPLSPPPPSSAPAASASR
FT M (in isoform 1)"
FT /evidence="ECO:0000303|PubMed:10718198"
FT /id="VSP_026469"
FT VAR_SEQ 514..602
FT /note="IMPSSPPLAAASSMSLPAAAPTTSVFSFSPVNMISAVKQRSAFAPVLRPPSS
FT PPQACPRAHGEGLPDQSFEDSDKFHSPARGLQGLAYS -> KERLRPRAAPPKLPTPGL
FT PQSPRRGASRPVF (in isoform 1)"
FT /evidence="ECO:0000303|PubMed:10718198"
FT /id="VSP_026470"
FT CONFLICT 425
FT /note="P -> S (in Ref. 2; BAA92680)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 602 AA; 64473 MW; EA340606A71094C1 CRC64;
MFPAQDALPR SGLNLKEEPL LPAGLGSVRS WMQGAGILDA STAAQSGVGL ARAHFEKQPP
SNLRKSNFFH FVLAMYDRQG QPVEVERTAF IDFVEKDREP GAEKTNNGIH YRLRLVYNNG
LRTEQDLYVR LIDSMSKQAI IYEGQDKNPE MCRVLLTHEI MCSRCCDRKS CGNRNETPSD
PVIIDRFFLK FFLKCNQNCL KNAGNPRDMR RFQVVVSTTV SVDGHVLAVS DNMFVHNNSK
HGRRARRLDP SEAATPCIKA ISPGEGWTTG GATVIVIGDN FFDGLQVVFG NVLVWSELIT
PHAIRVQTPP RHIPGVVEVT LSYKSKQFCK GCPGRFVYTA LNEPTIDYGF QRLQKVIPRH
PGDPERLPKE VLLKRAADLA EALYGVPGSN QELLLKRAAD VAEALYSTPR APGPLAPLAP
SHPHPAVVGI NAFSSPLAIA VGDATPGPEP GYARSCSSAS PRGFAPSPGS QQSGYGGGLG
AGLGGYGAPG VAGLGVPGSP SFLNGSTATS PFAIMPSSPP LAAASSMSLP AAAPTTSVFS
FSPVNMISAV KQRSAFAPVL RPPSSPPQAC PRAHGEGLPD QSFEDSDKFH SPARGLQGLA
YS
