COE4_MOUSE
ID COE4_MOUSE Reviewed; 599 AA.
AC Q8K4J2; A2BI82; A2BI83; Q8K4J1; Q8K4J3; Q8K4J4; Q8K4J5;
DT 28-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Transcription factor COE4;
DE AltName: Full=Early B-cell factor 4;
DE Short=EBF-4;
DE AltName: Full=Olf-1/EBF-like 4;
DE Short=O/E-4;
DE Short=OE-4;
GN Name=Ebf4; Synonyms=Coe4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 5), AND TISSUE
RP SPECIFICITY.
RC STRAIN=C57BL/6J;
RX PubMed=12139918; DOI=10.1006/mcne.2002.1138;
RA Wang S.S., Betz A.G., Reed R.R.;
RT "Cloning of a novel Olf-1/EBF-like gene, O/E-4, by degenerate oligo-based
RT direct selection.";
RL Mol. Cell. Neurosci. 20:404-414(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Seems to weakly activate transcription. Binds an Olf-1
CC consensus site in vitro.
CC -!- SUBUNIT: Forms either a homodimer or a heterodimer with a related
CC family member.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=3; Synonyms=4-23;
CC IsoId=Q8K4J2-1; Sequence=Displayed;
CC Name=1; Synonyms=4-11;
CC IsoId=Q8K4J2-2; Sequence=VSP_001125, VSP_001126;
CC Name=2; Synonyms=4-14;
CC IsoId=Q8K4J2-3; Sequence=VSP_001121, VSP_001122;
CC Name=4; Synonyms=4-132;
CC IsoId=Q8K4J2-4; Sequence=VSP_001123, VSP_001124;
CC Name=5; Synonyms=4S;
CC IsoId=Q8K4J2-5; Sequence=VSP_001119, VSP_001120;
CC -!- TISSUE SPECIFICITY: Expressed in the neuronal and basal cell layers of
CC olfactory epithelium. Absent in the vomeronasal organ.
CC {ECO:0000269|PubMed:12139918}.
CC -!- MISCELLANEOUS: [Isoform 4]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the COE family. {ECO:0000305}.
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DR EMBL; AF387630; AAM97580.1; -; mRNA.
DR EMBL; AF387631; AAM97581.1; -; mRNA.
DR EMBL; AF387632; AAM97582.1; -; mRNA.
DR EMBL; AF387633; AAM97583.1; -; mRNA.
DR EMBL; AF387634; AAM97584.1; -; mRNA.
DR EMBL; BX890605; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX936285; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS50709.1; -. [Q8K4J2-1]
DR RefSeq; NP_001103983.1; NM_001110513.1. [Q8K4J2-1]
DR RefSeq; XP_011237773.1; XM_011239471.2. [Q8K4J2-2]
DR RefSeq; XP_011237774.1; XM_011239472.2. [Q8K4J2-3]
DR AlphaFoldDB; Q8K4J2; -.
DR SMR; Q8K4J2; -.
DR BioGRID; 230746; 2.
DR STRING; 10090.ENSMUSP00000105915; -.
DR iPTMnet; Q8K4J2; -.
DR PhosphoSitePlus; Q8K4J2; -.
DR MaxQB; Q8K4J2; -.
DR PaxDb; Q8K4J2; -.
DR PRIDE; Q8K4J2; -.
DR ProteomicsDB; 283479; -. [Q8K4J2-1]
DR ProteomicsDB; 283480; -. [Q8K4J2-2]
DR ProteomicsDB; 283481; -. [Q8K4J2-3]
DR ProteomicsDB; 283482; -. [Q8K4J2-4]
DR ProteomicsDB; 283483; -. [Q8K4J2-5]
DR Antibodypedia; 23277; 91 antibodies from 17 providers.
DR DNASU; 228598; -.
DR Ensembl; ENSMUST00000110286; ENSMUSP00000105915; ENSMUSG00000053552. [Q8K4J2-1]
DR Ensembl; ENSMUST00000126740; ENSMUSP00000133528; ENSMUSG00000053552. [Q8K4J2-2]
DR Ensembl; ENSMUST00000140169; ENSMUSP00000134520; ENSMUSG00000053552. [Q8K4J2-4]
DR GeneID; 228598; -.
DR KEGG; mmu:228598; -.
DR UCSC; uc008miq.2; mouse. [Q8K4J2-1]
DR CTD; 57593; -.
DR MGI; MGI:2385972; Ebf4.
DR VEuPathDB; HostDB:ENSMUSG00000053552; -.
DR eggNOG; KOG3836; Eukaryota.
DR GeneTree; ENSGT00950000182859; -.
DR InParanoid; Q8K4J2; -.
DR OMA; QPGYARS; -.
DR TreeFam; TF313391; -.
DR BioGRID-ORCS; 228598; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q8K4J2; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8K4J2; protein.
DR Bgee; ENSMUSG00000053552; Expressed in olfactory epithelium and 120 other tissues.
DR ExpressionAtlas; Q8K4J2; baseline and differential.
DR Genevisible; Q8K4J2; MM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IMP:NTNU_SB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:MGI.
DR CDD; cd11606; COE_DBD; 1.
DR CDD; cd01175; IPT_COE; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 2.60.40.3180; -; 1.
DR InterPro; IPR032200; COE_DBD.
DR InterPro; IPR038173; COE_DBD_sf.
DR InterPro; IPR032201; COE_HLH.
DR InterPro; IPR038006; COE_IPT.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR002909; IPT_dom.
DR InterPro; IPR003523; Transcription_factor_COE.
DR InterPro; IPR018350; Transcription_factor_COE_CS.
DR PANTHER; PTHR10747; PTHR10747; 1.
DR Pfam; PF16422; COE1_DBD; 1.
DR Pfam; PF16423; COE1_HLH; 1.
DR Pfam; PF01833; TIG; 1.
DR SMART; SM00429; IPT; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
DR PROSITE; PS01345; COE; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Developmental protein; DNA-binding;
KW Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..599
FT /note="Transcription factor COE4"
FT /id="PRO_0000107836"
FT DOMAIN 256..339
FT /note="IPT/TIG"
FT ZN_FING 152..171
FT /note="C5-type"
FT /evidence="ECO:0000255"
FT REGION 64..67
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT REGION 198..205
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT REGION 237..240
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT REGION 449..473
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 556..586
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 164
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT SITE 173
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250"
FT VAR_SEQ 392..426
FT /note="ELLLKRAADVAEALYSAPRAPAPLGPLAPSHPHPA -> VWRLCPPPSARGR
FT GSDPAPAAAPAVPRSCLRRSSS (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:12139918"
FT /id="VSP_001119"
FT VAR_SEQ 427..599
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:12139918"
FT /id="VSP_001120"
FT VAR_SEQ 481..508
FT /note="GSYGAPGVTGLGVPGSPSFLNGSTATSP -> APRWRLPPPCPFRPPPPPPA
FT SSPSRLST (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12139918"
FT /id="VSP_001121"
FT VAR_SEQ 509..599
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12139918"
FT /id="VSP_001122"
FT VAR_SEQ 511..541
FT /note="IMPSSPPLAAASSMSLPAAAPTTSVFSFSPV -> KERLRPCAAPTQFPIAG
FT LPQSPQRGASRPAF (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:12139918"
FT /id="VSP_001123"
FT VAR_SEQ 542..599
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:12139918"
FT /id="VSP_001124"
FT VAR_SEQ 577..582
FT /note="DQPFED -> AQRTGR (in isoform 1)"
FT /evidence="ECO:0000303|PubMed:12139918"
FT /id="VSP_001125"
FT VAR_SEQ 583..599
FT /note="Missing (in isoform 1)"
FT /evidence="ECO:0000303|PubMed:12139918"
FT /id="VSP_001126"
SQ SEQUENCE 599 AA; 64624 MW; 76748B3E04D42260 CRC64;
MFPAQDALPR GGLHLKEEPL LPSSLGSVRS WMQSAGILDS NTAAQSGVGL ARAHFEKQPP
SNLRKSNFFH FVLAMYDRQG QPVEVERTAF IDFVEKDREP GTEKTNNGIH YRLRLVYNNG
LRTEQDLYVR LIDSMSKQAI IYEGQDKNPE MCRVLLTHEI MCSRCCDRKS CGNRNETPSD
PVIIDRFFLK FFLKCNQNCL KNAGNPRDMR RFQVVVSTTV SVDGHVLAVS DNMFVHNNSK
HGRRARRLDP SEAATPCIKA ISPGEGWTTG GATVIIIGDN FFDGLQVVFG NVLLWSELIT
PHAIRVQTPP RHIPGVVEVT LSYKSKQFCK GAPGRFVYTA LNEPTIDYGF QRLQKVIPRH
PGDPERLPKE VLLKRAADLA EALYGVPSSN QELLLKRAAD VAEALYSAPR APAPLGPLAP
SHPHPAVVGI NAFSSPLAIA VGDTTPEPGY ARSCGSASPR FAPSPGSQQS SYGSGLGAGL
GSYGAPGVTG LGVPGSPSFL NGSTATSPFA IMPSSPPLAA ASSMSLPAAA PTTSVFSFSP
VNMICAVKQR SAFAPVLRPP SSPSQACPRA HREGLPDQPF EDTDKFHSAA RGLQGLAYS
