ACP4_ARATH
ID ACP4_ARATH Reviewed; 137 AA.
AC Q9SW21;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 135.
DE RecName: Full=Acyl carrier protein 4, chloroplastic;
DE Flags: Precursor;
GN Name=ACP4; Synonyms=ACL1.4; OrderedLocusNames=At4g25050;
GN ORFNames=F13M23.190;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INDUCTION.
RX PubMed=11788768; DOI=10.1104/pp.010397;
RA Bonaventure G., Ohlrogge J.B.;
RT "Differential regulation of mRNA levels of acyl carrier protein isoforms in
RT Arabidopsis.";
RL Plant Physiol. 128:223-235(2002).
RN [6]
RP FUNCTION.
RX PubMed=12805604; DOI=10.1104/pp.102.018622;
RA Branen J.K., Shintani D.K., Engeseth N.J.;
RT "Expression of antisense acyl carrier protein-4 reduces lipid content in
RT Arabidopsis leaf tissue.";
RL Plant Physiol. 132:748-756(2003).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19218086; DOI=10.1016/j.chom.2009.01.001;
RA Xia Y., Gao Q.M., Yu K., Lapchyk L., Navarre D., Hildebrand D., Kachroo A.,
RA Kachroo P.;
RT "An intact cuticle in distal tissues is essential for the induction of
RT systemic acquired resistance in plants.";
RL Cell Host Microbe 5:151-165(2009).
CC -!- FUNCTION: Carrier of the growing fatty acid chain in fatty acid
CC biosynthesis that plays a major role in the biosynthesis of fatty acids
CC in leaves. Required for the biosynthesis of chloroplast photosynthetic
CC membrane lipids such as monogalactosyldiacylglycerol,
CC digalactosyldiacylglycerol and phosphatidylglycerol. Is essential for
CC the biosynthesis of the cuticular wax and cutin polymers in leaves, and
CC for the establishment of systemic acquired resistance (SAR).
CC {ECO:0000269|PubMed:12805604, ECO:0000269|PubMed:19218086}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9SW21-1; Sequence=Displayed;
CC -!- INDUCTION: By light. Down-regulated by sucrose in the dark.
CC {ECO:0000269|PubMed:11788768}.
CC -!- PTM: 4'-phosphopantetheine is transferred from CoA to a specific serine
CC of apo-ACP by acpS. This modification is essential for activity because
CC fatty acids are bound in thioester linkage to the sulfhydryl of the
CC prosthetic group (By similarity). {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Small and chlorotic plants with altered cuticule
CC and reduced levels of fatty acids in leaves.
CC {ECO:0000269|PubMed:19218086}.
CC -!- SIMILARITY: Belongs to the acyl carrier protein (ACP) family.
CC {ECO:0000305}.
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DR EMBL; AL035523; CAB36747.1; -; Genomic_DNA.
DR EMBL; AL161562; CAB79414.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84996.1; -; Genomic_DNA.
DR EMBL; AY039527; AAK62583.1; -; mRNA.
DR EMBL; AY050471; AAK91484.1; -; mRNA.
DR EMBL; AY084704; AAM61278.1; -; mRNA.
DR PIR; T05526; T05526.
DR RefSeq; NP_194235.1; NM_118637.4. [Q9SW21-1]
DR AlphaFoldDB; Q9SW21; -.
DR SMR; Q9SW21; -.
DR IntAct; Q9SW21; 1.
DR STRING; 3702.AT4G25050.2; -.
DR PRIDE; Q9SW21; -.
DR ProMEX; Q9SW21; -.
DR ProteomicsDB; 244360; -. [Q9SW21-1]
DR EnsemblPlants; AT4G25050.1; AT4G25050.1; AT4G25050. [Q9SW21-1]
DR GeneID; 828608; -.
DR Gramene; AT4G25050.1; AT4G25050.1; AT4G25050. [Q9SW21-1]
DR KEGG; ath:AT4G25050; -.
DR Araport; AT4G25050; -.
DR eggNOG; KOG1748; Eukaryota.
DR InParanoid; Q9SW21; -.
DR PhylomeDB; Q9SW21; -.
DR PRO; PR:Q9SW21; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SW21; baseline and differential.
DR Genevisible; Q9SW21; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000036; F:acyl carrier activity; IEA:InterPro.
DR GO; GO:0042335; P:cuticle development; IMP:UniProtKB.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IMP:UniProtKB.
DR GO; GO:0009627; P:systemic acquired resistance; IMP:UniProtKB.
DR Gene3D; 1.10.1200.10; -; 1.
DR HAMAP; MF_01217; Acyl_carrier; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR044813; ACP_chloroplastic.
DR InterPro; IPR003231; Acyl_carrier.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR PANTHER; PTHR46153; PTHR46153; 1.
DR Pfam; PF00550; PP-binding; 1.
DR SUPFAM; SSF47336; SSF47336; 1.
DR TIGRFAMs; TIGR00517; acyl_carrier; 1.
DR PROSITE; PS50075; CARRIER; 1.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Fatty acid biosynthesis;
KW Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism;
KW Phosphopantetheine; Phosphoprotein; Plant defense; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..48
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 49..137
FT /note="Acyl carrier protein 4, chloroplastic"
FT /id="PRO_0000418156"
FT DOMAIN 53..128
FT /note="Carrier"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 88
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 137 AA; 14544 MW; 326F81C96714A5DF CRC64;
MASLSTTSLS FKAPSTTISQ VLRKASSSQS VTFGRFTSST KSLRLQISCA AKAETVQKVS
DIVKEQLALA ADVPLTAESK FSALGADSLD TVEIVMALEE KFNISVEESD AQNITTIQEA
ADLIEDLVQK KPAAETS
