ID   ACPA_BACAN              Reviewed;         483 AA.
AC   Q44643;
DT   02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 128.
DE   RecName: Full=Capsule synthesis positive regulator AcpA;
GN   Name=acpA; OrderedLocusNames=pXO2-64, BXB0084, GBAA_pXO2_0084;
OS   Bacillus anthracis.
OG   Plasmid pXO2.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7828912; DOI=10.1016/0378-1119(94)00662-c;
RA   Vietri N.J., Marrero R., Hoover T.A., Welkos S.L.;
RT   "Identification and characterization of a trans-activator involved in the
RT   regulation of encapsulation by Bacillus anthracis.";
RL   Gene 152:1-9(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Pasteur;
RX   PubMed=10475962; DOI=10.1046/j.1365-2672.1999.00883.x;
RA   Okinaka R.T., Cloud K., Hampton O., Hoffmaster A., Hill K.K., Keim P.,
RA   Koehler T., Lamke G., Kumano S., Manter D., Martinez Y., Ricke D.,
RA   Svensson R., Jackson P.J.;
RT   "Sequence, assembly and analysis of pXO1 and pXO2.";
RL   J. Appl. Microbiol. 87:261-262(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Ames / isolate Florida / A2012;
RX   PubMed=12004073; DOI=10.1126/science.1071837;
RA   Read T.D., Salzberg S.L., Pop M., Shumway M.F., Umayam L., Jiang L.,
RA   Holtzapple E., Busch J.D., Smith K.L., Schupp J.M., Solomon D., Keim P.,
RA   Fraser C.M.;
RT   "Comparative genome sequencing for discovery of novel polymorphisms in
RT   Bacillus anthracis.";
RL   Science 296:2028-2033(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor;
RX   PubMed=18952800; DOI=10.1128/jb.01347-08;
RA   Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA   Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT   "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL   J. Bacteriol. 191:445-446(2009).
RN   [5]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=14702298; DOI=10.1128/jb.186.2.307-315.2004;
RA   Drysdale M., Bourgogne A., Hilsenbeck S.G., Koehler T.M.;
RT   "atxA controls Bacillus anthracis capsule synthesis via acpA and a newly
RT   discovered regulator, acpB.";
RL   J. Bacteriol. 186:307-315(2004).
CC   -!- FUNCTION: AcpA and AcpB regulate cap gene expression and capsule
CC       synthesis. {ECO:0000269|PubMed:14702298}.
CC   -!- INDUCTION: Transcriptionally regulated by AtxA. Transcription is also
CC       positively controlled by carbonic acid and CO(2).
CC       {ECO:0000269|PubMed:14702298}.
CC   -!- MISCELLANEOUS: AcpA has a larger effect on cap gene expression than
CC       does AcpB.
CC   -!- SIMILARITY: Belongs to the AtxA/AcpA family. {ECO:0000305}.
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DR   EMBL; U02535; AAA81894.1; -; Genomic_DNA.
DR   EMBL; AF188935; AAF13669.1; -; Genomic_DNA.
DR   EMBL; AE011191; AAM26236.1; -; Genomic_DNA.
DR   EMBL; AE017335; AAT29014.2; -; Genomic_DNA.
DR   PIR; I40055; I40055.
DR   RefSeq; NP_053219.1; NC_002146.1.
DR   RefSeq; WP_000409768.1; NZ_VTZL01000009.1.
DR   AlphaFoldDB; Q44643; -.
DR   SMR; Q44643; -.
DR   IntAct; Q44643; 2.
DR   EnsemblBacteria; AAT29014; AAT29014; GBAA_pXO2_0084.
DR   GeneID; 45025376; -.
DR   KEGG; bar:GBAA_pXO2_0084; -.
DR   HOGENOM; CLU_044310_0_0_9; -.
DR   OMA; HTFAISI; -.
DR   PHI-base; PHI:4088; -.
DR   Proteomes; UP000000594; Plasmid pXO2.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR013199; HTH_Mga_DNA-bd_dom.
DR   InterPro; IPR007737; Mga_HTH.
DR   InterPro; IPR011608; PRD.
DR   InterPro; IPR036634; PRD_sf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF08280; HTH_Mga; 1.
DR   Pfam; PF05043; Mga; 1.
DR   Pfam; PF00874; PRD; 1.
DR   SUPFAM; SSF63520; SSF63520; 1.
DR   PROSITE; PS51372; PRD_2; 2.
PE   2: Evidence at transcript level;
KW   Activator; Capsule biogenesis/degradation; Plasmid; Reference proteome;
KW   Repeat; Transcription; Transcription regulation; Virulence.
FT   CHAIN           1..483
FT                   /note="Capsule synthesis positive regulator AcpA"
FT                   /id="PRO_0000219546"
FT   DOMAIN          165..270
FT                   /note="PRD 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT   DOMAIN          283..394
FT                   /note="PRD 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
SQ   SEQUENCE   483 AA;  57035 MW;  0CB21A651A079F4E CRC64;
     MEKDISRKID LLNILIEEKR WFTLFELEKN LNCSSKTIRK DISIINDLLP KTIFIHSKKG
     KGVKLSLPQN QSISEAISNL LKKSLTFLAI QQLLEERSNT VTSLADKLYL PISSTNIVLK
     RVSKYIKKFG LSLEKKPLRI VGDEFQIILM FSERYLESYP DTEWPFTEYK EEMLIDYINY
     IEEKLEIVFY SNDKRRMAFI MTILFKRIKQ GHKVKFSEWI IKETMESIYY KKIFEGKNVI
     KVNKNRSLNI EEQVLLVIMV KLSRYVSKDE NNLKQEELVL YKEGESTTYT YVKNFISILE
     QELKIDLNNN EEFVYGMIEY CREAFHILKF IPILKAPEKD TCKYIKKHYE ETFYLVKRAY
     NKWGAEMKLT DIPDEEIAKV TMRIVAIGKQ HNINRKKVLL ITGEGKSWEE YMKSRINKRY
     GDQLKFVGGH AKILNGNTDN IDNIDIDFII TTVPLNFSWK SIVYVSPILQ ERDFYEIGIF
     ASK