ACPA_EMENI
ID ACPA_EMENI Reviewed; 298 AA.
AC Q5B2K4; C8VFA3;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Acetate permease A {ECO:0000303|PubMed:18302536};
DE AltName: Full=Monocarboxylate transporter acpA {ECO:0000303|PubMed:18302536};
GN Name=acpA {ECO:0000303|PubMed:18302536};
GN ORFNames=AN5226.2 {ECO:0000303|PubMed:19146970};
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [3]
RP INDUCTION.
RX PubMed=16531087; DOI=10.1016/j.fgb.2005.12.008;
RA Flipphi M., Robellet X., Dequier E., Leschelle X., Felenbok B., Velot C.;
RT "Functional analysis of alcS, a gene of the alc cluster in Aspergillus
RT nidulans.";
RL Fungal Genet. Biol. 43:247-260(2006).
RN [4]
RP DISRUPTION PHENOTYPE, FUNCTION, AND INDUCTION.
RX PubMed=18302536; DOI=10.1042/bj20080124;
RA Robellet X., Flipphi M., Pegot S., Maccabe A.P., Velot C.;
RT "AcpA, a member of the GPR1/FUN34/YaaH membrane protein family, is
RT essential for acetate permease activity in the hyphal fungus Aspergillus
RT nidulans.";
RL Biochem. J. 412:485-493(2008).
CC -!- FUNCTION: High affinity monocarboxylate transporter (MCT) involved in
CC acetate uptake. Unlike other activities involved in acetate
CC utilization, acpA is dispensable for growth on the acetate precursor
CC ethanol. {ECO:0000269|PubMed:18302536, ECO:0000303|PubMed:16531087}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P25613};
CC Multi-pass membrane protein {ECO:0000255}. Vacuole membrane
CC {ECO:0000250|UniProtKB:P25613}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: Expression is induced by ethanol and ethyl acetate and is
CC mediated by the specific transcriptional activator of genes of the
CC acetate utilization pathway facB. Expression is also induced by low
CC concentrations of acetate or propionate and under the controle of the
CC facB transcription activator. {ECO:0000269|PubMed:16531087,
CC ECO:0000269|PubMed:18302536}.
CC -!- DISRUPTION PHENOTYPE: Leads to reduced growth when acetate is used as
CC sole carbon source at low concentration and in a high pH medium.
CC {ECO:0000269|PubMed:18302536}.
CC -!- SIMILARITY: Belongs to the acetate uptake transporter (AceTr) (TC
CC 2.A.96) family. {ECO:0000305}.
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DR EMBL; BN001305; CBF81120.1; -; Genomic_DNA.
DR EMBL; AACD01000089; EAA62407.1; -; Genomic_DNA.
DR RefSeq; XP_662830.1; XM_657738.1.
DR AlphaFoldDB; Q5B2K4; -.
DR SMR; Q5B2K4; -.
DR STRING; 162425.CADANIAP00003225; -.
DR TCDB; 2.A.96.1.3; the acetate uptake transporter (acetr) family.
DR EnsemblFungi; CBF81120; CBF81120; ANIA_05226.
DR EnsemblFungi; EAA62407; EAA62407; AN5226.2.
DR GeneID; 2871510; -.
DR KEGG; ani:AN5226.2; -.
DR VEuPathDB; FungiDB:AN5226; -.
DR eggNOG; ENOG502QUJS; Eukaryota.
DR HOGENOM; CLU_051062_0_1_1; -.
DR InParanoid; Q5B2K4; -.
DR OMA; WKKGNTF; -.
DR OrthoDB; 1117364at2759; -.
DR Proteomes; UP000000560; Chromosome V.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015123; F:acetate transmembrane transporter activity; IMP:AspGD.
DR GO; GO:0006068; P:ethanol catabolic process; IEP:AspGD.
DR GO; GO:0006847; P:plasma membrane acetate transport; IMP:AspGD.
DR InterPro; IPR000791; Gpr1/Fun34/SatP.
DR Pfam; PF01184; Gpr1_Fun34_YaaH; 1.
DR PROSITE; PS01114; GPR1_FUN34_YAAH; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..298
FT /note="Acetate permease A"
FT /id="PRO_0000430707"
FT TRANSMEM 86..106
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REGION 1..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 298 AA; 31646 MW; 45729F845705ABA7 CRC64;
MSAEQNHGLE KDVGGPAAPA AAAPNAPAAA PGAPPAGMSA EEHRSRFGYG PLSHVNTKEA
ILPPFGGEFQ PGLYKSVEAR KFANPAPLGL SAFALTTFVL SCINMGARDI THPNIVIALA
FGYGGLVQLL AGMWEMAVGN TFGATALSSY GGFWIAFAIV LTPGGFNIQT ALTAENGDEA
MFYNSFGLFL MGWFIFTTIM LFCTLRSTVA FFLLFLFLDL AFLLLGVGYI QRDDAGQPNP
PVIKAGGFFG LLAAFAAWYN ALAGIADSSN SFFIIPVAHF PWSPTGRARR EKTERETV
