COG3_YEAST
ID COG3_YEAST Reviewed; 801 AA.
AC P40094; D3DM64;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Conserved oligomeric Golgi complex subunit 3;
DE Short=COG complex subunit 3;
DE AltName: Full=Component of oligomeric Golgi complex 3;
DE AltName: Full=Protein SEC34;
GN Name=COG3; Synonyms=GRD20, SEC34; OrderedLocusNames=YER157W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=10562277; DOI=10.1083/jcb.147.4.729;
RA VanRheenen S.M., Cao X., Sapperstein S.K., Chiang E.C., Lupashin V.V.,
RA Barlowe C., Waters M.G.;
RT "Sec34p, a protein required for vesicle tethering to the yeast Golgi
RT apparatus, is in a complex with Sec35p.";
RL J. Cell Biol. 147:729-742(1999).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10588657; DOI=10.1091/mbc.10.12.4263;
RA Spelbrink R.G., Nothwehr S.F.;
RT "The yeast GRD20 gene is required for protein sorting in the trans-Golgi
RT network/endosomal system and for polarization of the actin cytoskeleton.";
RL Mol. Biol. Cell 10:4263-4281(1999).
RN [5]
RP SUBUNIT.
RX PubMed=11703943; DOI=10.1016/s1534-5807(01)00063-6;
RA Whyte J.R., Munro S.;
RT "The Sec34/35 Golgi transport complex is related to the exocyst, defining a
RT family of complexes involved in multiple steps of membrane traffic.";
RL Dev. Cell 1:527-537(2001).
RN [6]
RP FUNCTION, AND IDENTIFICATION IN THE COG COMPLEX.
RX PubMed=12006647; DOI=10.1091/mbc.01-10-0495;
RA Ram R.J., Li B., Kaiser C.A.;
RT "Identification of sec36p, sec37p, and sec38p: components of yeast complex
RT that contains sec34p and sec35p.";
RL Mol. Biol. Cell 13:1484-1500(2002).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP COMPOSITION OF THE COG COMPLEX, AND INTERACTION WITH COG1 AND COG2.
RX PubMed=15047703; DOI=10.1074/jbc.m400662200;
RA Loh E., Hong W.;
RT "The binary interacting network of the conserved oligomeric Golgi tethering
RT complex.";
RL J. Biol. Chem. 279:24640-24648(2004).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-507 AND SER-647, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-647, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Acts as component of the peripheral membrane COG complex that
CC is involved in intra-Golgi protein trafficking. COG is located at the
CC cis- Golgi, and regulates tethering of retrograde intra-Golgi vesicles
CC and possibly a number of other membrane trafficking events. COG3 is
CC also involved in actin cytoskeleton organization.
CC {ECO:0000269|PubMed:10562277, ECO:0000269|PubMed:10588657,
CC ECO:0000269|PubMed:12006647}.
CC -!- SUBUNIT: Component of the conserved oligomeric Golgi (COG or
CC Sec34/Sec35) complex which consists of eight different proteins COG1-
CC COG8. {ECO:0000269|PubMed:10562277, ECO:0000269|PubMed:11703943,
CC ECO:0000269|PubMed:12006647}.
CC -!- INTERACTION:
CC P40094; P53079: COG1; NbExp=12; IntAct=EBI-16605, EBI-4835;
CC P40094; P53271: COG2; NbExp=16; IntAct=EBI-16605, EBI-16614;
CC P40094; Q06096: COG4; NbExp=4; IntAct=EBI-16605, EBI-4823;
CC P40094; P53951: COG5; NbExp=3; IntAct=EBI-16605, EBI-4841;
CC P40094; P53959: COG6; NbExp=3; IntAct=EBI-16605, EBI-4829;
CC P40094; P53195: COG7; NbExp=2; IntAct=EBI-16605, EBI-4847;
CC P40094; Q01590: SED5; NbExp=4; IntAct=EBI-16605, EBI-16930;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:10562277, ECO:0000269|PubMed:10588657}; Peripheral
CC membrane protein {ECO:0000269|PubMed:10562277,
CC ECO:0000269|PubMed:10588657}; Cytoplasmic side
CC {ECO:0000269|PubMed:10562277, ECO:0000269|PubMed:10588657}.
CC -!- MISCELLANEOUS: Present with 4140 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the COG3 family. {ECO:0000305}.
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DR EMBL; U18917; AAB64684.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07818.1; -; Genomic_DNA.
DR PIR; S50660; S50660.
DR RefSeq; NP_011084.1; NM_001179047.1.
DR AlphaFoldDB; P40094; -.
DR SMR; P40094; -.
DR BioGRID; 36907; 1029.
DR ComplexPortal; CPX-1840; COG Golgi transport complex.
DR DIP; DIP-4728N; -.
DR IntAct; P40094; 24.
DR MINT; P40094; -.
DR STRING; 4932.YER157W; -.
DR iPTMnet; P40094; -.
DR MaxQB; P40094; -.
DR PaxDb; P40094; -.
DR PRIDE; P40094; -.
DR EnsemblFungi; YER157W_mRNA; YER157W; YER157W.
DR GeneID; 856901; -.
DR KEGG; sce:YER157W; -.
DR SGD; S000000959; COG3.
DR VEuPathDB; FungiDB:YER157W; -.
DR eggNOG; KOG2604; Eukaryota.
DR GeneTree; ENSGT00390000015682; -.
DR HOGENOM; CLU_011639_2_0_1; -.
DR InParanoid; P40094; -.
DR OMA; LDEFELW; -.
DR BioCyc; YEAST:G3O-30318-MON; -.
DR PRO; PR:P40094; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P40094; protein.
DR GO; GO:0005801; C:cis-Golgi network; IEA:InterPro.
DR GO; GO:0000139; C:Golgi membrane; IC:ComplexPortal.
DR GO; GO:0017119; C:Golgi transport complex; IMP:SGD.
DR GO; GO:0140312; F:cargo adaptor activity; IMP:SGD.
DR GO; GO:0006914; P:autophagy; IBA:GO_Central.
DR GO; GO:0030242; P:autophagy of peroxisome; IMP:SGD.
DR GO; GO:0032258; P:cytoplasm to vacuole transport by the Cvt pathway; IMP:SGD.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IGI:SGD.
DR GO; GO:0007030; P:Golgi organization; IBA:GO_Central.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IMP:SGD.
DR GO; GO:0016236; P:macroautophagy; IMP:SGD.
DR GO; GO:0000301; P:retrograde transport, vesicle recycling within Golgi; IMP:SGD.
DR InterPro; IPR007265; COG_su3.
DR PANTHER; PTHR13302; PTHR13302; 1.
DR Pfam; PF04136; Sec34; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Membrane; Phosphoprotein; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..801
FT /note="Conserved oligomeric Golgi complex subunit 3"
FT /id="PRO_0000213503"
FT REGION 470..489
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 507
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 647
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:19779198"
SQ SEQUENCE 801 AA; 92544 MW; C7AFBE99010FE571 CRC64;
MARSRKNSLV RDIASHPTIP ESQTIVGLLD DSYLFDKLKK LSLAVENSDS LQRTDVSEGC
SEVNGSEATT SADVKKTNKY LYYTTYLDQL NIKIDEYKVV LDQTRQVNDQ LDSSIKKFRK
ISQDTGAFIE ETKTIYEKQS KLSNLTESIP KALHYFEVLD PIMRRLNHAT SPAIVKKSSF
TTMLATIDES LRFLDENSDL KDAAAYRIKF KQCLIRACEL ISHFLTNLLK QTNQEILDKT
KNKNSLTGLP STTRDAFLYS KFYTIADTFK IQVSEIVKRS NEKAYNKYHD ELNSILYECF
NHYFQTRLRL LTPVIWSHID EIVVKDKDQG LVKFIQDGKV YFQQLCADEY KLFVEFFPEK
ECRFKINQWF LQLCEPLYDS IRVRVLKETD ICTLCDSVTL FAPYYEFEEG SEEYVKQFTD
IQYDKLFEPI VQKVQARLIL RVQIYVQQNI LSYRPTRDVF MISNRRRKSK TSLQGGNEDA
TTSDDNPDPL LESYLSSFKN RSILPISPND ADDKSIDSEE STDKISQLQT YYPPLLKTLA
LLSKIYEMIN SVVFDDLAHH VVHDCIVSLR NAYDMVIKSS AGKSDFNNLD ISLAYLKNLL
MLRDSIQNFN IQYTVNETYL DFSGVEGFFK SLKENGRNVL KKTKSSSILT LARELVPKVV
NNMVDARTEL ISELRNVIKD FTESTSLELI DDTLDINSDE DLLSKNVKLR ENIKARLPRI
YEQILNYIDD QEIVTNLLDA VQELITQSYS KYYETITELA ENGKFAKDQV ADVMYLDVFT
DFFAKEVADL LRNGDIDTIT K
