COG5_DROME
ID COG5_DROME Reviewed; 751 AA.
AC Q9VJD3; Q53YG5; Q8T0G3;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Conserved oligomeric Golgi complex subunit 5;
DE Short=COG complex subunit 5;
DE AltName: Full=Component of oligomeric Golgi complex 5;
DE AltName: Full=Protein four way stop;
GN Name=fws; ORFNames=CG6549;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 406-751.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=12529436; DOI=10.1091/mbc.e02-06-0343;
RA Farkas R.M., Giansanti M.G., Gatti M., Fuller M.T.;
RT "The Drosophila Cog5 homologue is required for cytokinesis, cell
RT elongation, and assembly of specialized Golgi architecture during
RT spermatogenesis.";
RL Mol. Biol. Cell 14:190-200(2003).
CC -!- FUNCTION: Required for normal Golgi function and necessary during
CC spermatogenesis. Required for cleavage furrow ingression during
CC cytokinesis in dividing spermatocytes and for the extensive polarized
CC cell growth that accompanies spermatid elongation.
CC {ECO:0000269|PubMed:12529436}.
CC -!- SUBUNIT: Component of the conserved oligomeric Golgi complex which is
CC composed of eight different subunits and is required for normal Golgi
CC morphology and localization. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9VJD3; Q4QPW4: Dmel\CG13171; NbExp=4; IntAct=EBI-99764, EBI-15141584;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:12529436}; Peripheral membrane protein
CC {ECO:0000269|PubMed:12529436}. Note=Localized to Golgi membrane
CC throughout spermatogenesis, as seen in spermatocytes and
CC differentiating spermatids.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos, and adult males and females.
CC {ECO:0000269|PubMed:12529436}.
CC -!- DISRUPTION PHENOTYPE: Males are sterile. {ECO:0000269|PubMed:12529436}.
CC -!- SIMILARITY: Belongs to the COG5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL39480.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014134; AAF53619.1; -; Genomic_DNA.
DR EMBL; AE014134; AAN10990.1; -; Genomic_DNA.
DR EMBL; BT003257; AAO25014.1; -; mRNA.
DR EMBL; AY069335; AAL39480.1; ALT_INIT; mRNA.
DR RefSeq; NP_001246059.1; NM_001259130.2.
DR RefSeq; NP_001286015.1; NM_001299086.1.
DR RefSeq; NP_001286016.1; NM_001299087.1.
DR RefSeq; NP_523590.1; NM_078866.4.
DR RefSeq; NP_724058.1; NM_165221.3.
DR AlphaFoldDB; Q9VJD3; -.
DR SMR; Q9VJD3; -.
DR BioGRID; 61050; 6.
DR DIP; DIP-17233N; -.
DR IntAct; Q9VJD3; 10.
DR STRING; 7227.FBpp0080552; -.
DR PaxDb; Q9VJD3; -.
DR PRIDE; Q9VJD3; -.
DR DNASU; 35054; -.
DR EnsemblMetazoa; FBtr0080999; FBpp0080552; FBgn0024689.
DR EnsemblMetazoa; FBtr0081000; FBpp0080553; FBgn0024689.
DR EnsemblMetazoa; FBtr0305685; FBpp0296965; FBgn0024689.
DR EnsemblMetazoa; FBtr0346626; FBpp0312206; FBgn0024689.
DR EnsemblMetazoa; FBtr0346627; FBpp0312207; FBgn0024689.
DR GeneID; 35054; -.
DR KEGG; dme:Dmel_CG6549; -.
DR UCSC; CG6549-RC; d. melanogaster.
DR CTD; 100529224; -.
DR FlyBase; FBgn0024689; fws.
DR VEuPathDB; VectorBase:FBgn0024689; -.
DR eggNOG; KOG2211; Eukaryota.
DR GeneTree; ENSGT00390000004586; -.
DR HOGENOM; CLU_009839_1_1_1; -.
DR InParanoid; Q9VJD3; -.
DR OMA; KSFDECP; -.
DR OrthoDB; 871605at2759; -.
DR PhylomeDB; Q9VJD3; -.
DR Reactome; R-DME-6807878; COPI-mediated anterograde transport.
DR Reactome; R-DME-6811438; Intra-Golgi traffic.
DR SignaLink; Q9VJD3; -.
DR BioGRID-ORCS; 35054; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35054; -.
DR PRO; PR:Q9VJD3; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0024689; Expressed in capitellum (Drosophila) and 27 other tissues.
DR ExpressionAtlas; Q9VJD3; baseline and differential.
DR Genevisible; Q9VJD3; DM.
DR GO; GO:0070971; C:endoplasmic reticulum exit site; IDA:FlyBase.
DR GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0017119; C:Golgi transport complex; IDA:FlyBase.
DR GO; GO:0001675; P:acrosome assembly; IMP:FlyBase.
DR GO; GO:0000916; P:actomyosin contractile ring contraction; IMP:FlyBase.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; ISS:FlyBase.
DR GO; GO:0007112; P:male meiosis cytokinesis; IMP:UniProtKB.
DR GO; GO:0007110; P:meiosis I cytokinesis; IMP:FlyBase.
DR GO; GO:0007111; P:meiosis II cytokinesis; IMP:FlyBase.
DR GO; GO:0000212; P:meiotic spindle organization; IMP:FlyBase.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007290; P:spermatid nucleus elongation; IMP:UniProtKB.
DR InterPro; IPR019465; Cog5.
DR PANTHER; PTHR13228; PTHR13228; 1.
DR Pfam; PF10392; COG5; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Golgi apparatus; Membrane;
KW Protein transport; Reference proteome; Spermatogenesis; Transport.
FT CHAIN 1..751
FT /note="Conserved oligomeric Golgi complex subunit 5"
FT /id="PRO_0000213511"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 244..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 751 AA; 84954 MW; E32B1D05BEEF6EBA CRC64;
MVTGDPVATK TPNAADSDDN DFTASMSHLT IGQQIQELSK QLQNTKEELH QQVRDKHGAL
LQQATHAGRF DAALNALAED VQRVRETGHR LKNQVDTQYQ QVENQTQVLG RLHDVSHLLR
SAGTLLSLTA KLKATKDVLR LAEIHFELGQ LIEDKELKDI DFIQQERAYV ISSAQKIRNL
TQMQLVTGLQ ERNENQVVNA LKIFMNFNTL EKSLDNLLAT FIADMEQSLK ECFAGNDISV
LNKSPTHNVS KPAPSRGPGK TPQLTTTQNF RAKFWKSLHW LLYDELFETC TQIKLLKTAL
EQINQFGYTS ESSDQCIPQR FWQQVQQLLR KSFDECPQHV TQTLQEGLSK LLTSARGLEQ
RLHGEFQFDN ELFAPLEVGY VSKCAANFKA CLAGVDLPGN ETVDNFIRVA STELSAALID
SRLTNAIANV FAACGKELCT KLEAQIKLGA DSKQVVDLPN LQQQQNTQLA NVLFYYKDSV
RRMLSDLHVH FEKTPGTARE IISRSLEQAD LLIGTILQQI MESIITTISI IVLSMHREPG
LNSERMSTTG PSMYMKELQE FVNRSWSHHI ALFDDKQMTK KCGHELAKRC IELFLHNVCI
LRPLSSCGRQ RLKQDCQHME QALKPLCPNL AELGKPSRLL RAMSLLIVQT AEELVKQTIG
EDSLVPSYIV LLLLFGHAGA DLQSPHTTAN WSNERLIEWL DGHTAEREKL ELISGALQRY
RDNARRKNIQ QYDEVYPMMV DYFEQALKAL P
