COG8_ARATH
ID COG8_ARATH Reviewed; 569 AA.
AC Q84K25; Q8LCN9; Q9LYH5;
DT 13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=Conserved oligomeric Golgi complex subunit 8 {ECO:0000303|PubMed:27448097};
DE Short=COG complex subunit 8 {ECO:0000303|PubMed:27448097};
DE AltName: Full=Component of oligomeric Golgi complex 8 {ECO:0000303|PubMed:27448097};
GN Name=COG8 {ECO:0000303|PubMed:27448097};
GN OrderedLocusNames=At5g11980 {ECO:0000312|Araport:AT5G11980};
GN ORFNames=F14F18.150 {ECO:0000312|EMBL:CAB87671.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION
RP WITH COG1; COG3; COG5 AND COG6, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=27448097; DOI=10.1371/journal.pgen.1006140;
RA Tan X., Cao K., Liu F., Li Y., Li P., Gao C., Ding Y., Lan Z., Shi Z.,
RA Rui Q., Feng Y., Liu Y., Zhao Y., Wu C., Zhang Q., Li Y., Jiang L., Bao Y.;
RT "Arabidopsis COG complex subunits COG3 and COG8 modulate golgi morphology,
RT vesicle trafficking homeostasis and are essential for pollen tube growth.";
RL PLoS Genet. 12:E1006140-E1006140(2016).
CC -!- FUNCTION: Acts as component of the peripheral membrane COG complex that
CC is involved in intra-Golgi protein trafficking (PubMed:27448097).
CC Involved in pollen tube growth by modulating Golgi morphology and
CC vesicle trafficking homeostasis leading to the deposition of cell wall
CC components and proteins at the pollen tube tip (PubMed:27448097).
CC Required for sporophytic development (PubMed:27448097).
CC {ECO:0000269|PubMed:27448097}.
CC -!- SUBUNIT: Homodimer (PubMed:27448097). Component of the conserved
CC oligomeric Golgi complex which is composed of eight different subunits
CC and is required for normal Golgi morphology and localization
CC (Probable). Interacts with COG1, COG3, COG5 and COG6 (PubMed:27448097).
CC {ECO:0000269|PubMed:27448097, ECO:0000305|PubMed:27448097}.
CC -!- INTERACTION:
CC Q84K25; P46639: KNAT1; NbExp=3; IntAct=EBI-4425470, EBI-530486;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:27448097}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q96MW5}.
CC -!- DISRUPTION PHENOTYPE: Male-specific transmission defect due to
CC incorrect deposition of cell wall components and proteins during pollen
CC tube elongation, thus leading to disrupted pollen tube growth
CC (PubMed:27448097). Embryo- and seedling-lethal associated with white
CC seeds in siliques, delayed embryo development from the early stages,
CC arrested at heart stages and resulting in abnormal curled cotyledons
CC (PubMed:27448097). Altered Golgi bodies morphology (PubMed:27448097).
CC Impaired Gamma-COP and TMN1/EMP12 tight association with the Golgi
CC (PubMed:27448097). {ECO:0000269|PubMed:27448097}.
CC -!- SIMILARITY: Belongs to the COG8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB87671.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL163812; CAB87671.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91747.1; -; Genomic_DNA.
DR EMBL; BT002913; AAO22729.1; -; mRNA.
DR EMBL; BT004349; AAO42343.1; -; mRNA.
DR EMBL; AY086485; AAM63487.1; -; mRNA.
DR PIR; T48557; T48557.
DR RefSeq; NP_568256.1; NM_121236.2.
DR AlphaFoldDB; Q84K25; -.
DR SMR; Q84K25; -.
DR IntAct; Q84K25; 22.
DR STRING; 3702.AT5G11980.1; -.
DR iPTMnet; Q84K25; -.
DR PaxDb; Q84K25; -.
DR PRIDE; Q84K25; -.
DR ProteomicsDB; 175273; -.
DR EnsemblPlants; AT5G11980.1; AT5G11980.1; AT5G11980.
DR GeneID; 831071; -.
DR Gramene; AT5G11980.1; AT5G11980.1; AT5G11980.
DR KEGG; ath:AT5G11980; -.
DR Araport; AT5G11980; -.
DR TAIR; locus:2143074; AT5G11980.
DR eggNOG; KOG2069; Eukaryota.
DR HOGENOM; CLU_017968_4_0_1; -.
DR InParanoid; Q84K25; -.
DR OrthoDB; 1531483at2759; -.
DR PhylomeDB; Q84K25; -.
DR PRO; PR:Q84K25; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q84K25; baseline and differential.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0017119; C:Golgi transport complex; IPI:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0007030; P:Golgi organization; IMP:TAIR.
DR GO; GO:0048193; P:Golgi vesicle transport; IMP:TAIR.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0009860; P:pollen tube growth; IMP:TAIR.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR007255; COG8.
DR InterPro; IPR016632; COG8_Metazoal_Plant.
DR InterPro; IPR016159; Cullin_repeat-like_dom_sf.
DR PANTHER; PTHR21311; PTHR21311; 1.
DR Pfam; PF04124; Dor1; 1.
DR PIRSF; PIRSF015415; COG8; 1.
DR SUPFAM; SSF74788; SSF74788; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Membrane; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..569
FT /note="Conserved oligomeric Golgi complex subunit 8"
FT /id="PRO_0000448530"
FT REGION 511..569
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 511..544
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 554..569
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 291
FT /note="W -> S (in Ref. 4; AAM63487)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 569 AA; 63052 MW; 318A063E54AC1AB6 CRC64;
MAMEVGEMSQ PEATASLLSL ASATQQPYVS ELLSFTLDRL HKEPELLRVD AERIQRQMQE
VAVGNYRAFI TAADALLAIR QEVSSIDKHL ESLIGEVPKL TSGCTEFIDS AENILEKRKM
NQALLANHST LLDLLEIPQL MDTCVRNGNF DEALDLEAFV SKLATLHPKL PVIQALAAEV
RQTTQSLLSQ LLQKLRSNIQ LPECLRIIGY LRRIGVFGEY EMRLQFLRCR EAWLTGILED
LDQKNAYEYL KGMINCHRMH LFDVVNQYRA IFSDDTSGSE ENYDGGLLFS WAMHQITSHL
KTLKIMLPKI TEGGSLSNIL DQCMYCAMGL GGVGLDFRGL LPPLFEEAVL NLFSKNMSTA
VENFQLVLDS HRWVPLPSVG FPSSGINEDS KDDVTPPSYL MEHPPLAVFI NGVSSALNEL
RPCAPLSLKN VVAHELIKGL QAVSDSLLRY NTTRMLRLSE SNLFLSLCRA FVEVVFPHCA
TCFGRCYPGG ATIVMDAKSA YEGLGRILAA SSSQEPSNKS PKVISTDTKD ASENGVASQP
EEKQAENPNA KEEDNSPIPL QTPEITPES
