COL2_ARATH
ID COL2_ARATH Reviewed; 347 AA.
AC Q96502;
DT 05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Zinc finger protein CONSTANS-LIKE 2;
GN Name=COL2; OrderedLocusNames=At3g02380;
GN ORFNames=F11A12.106, F11A12.7, F16B3.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Landsberg erecta; TISSUE=Aerial part;
RA Ledger S.E., Dare A.P., Putterill J.J.;
RT "COL2 is a homologue of the Arabidopsis flowering-time gene CONSTANS.";
RL (er) Plant Gene Register PGR96-081(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP CHARACTERIZATION.
RX PubMed=11359606; DOI=10.1046/j.1365-313x.2001.01003.x;
RA Ledger S.E., Strayer C., Ashton F., Kay S.A., Putterill J.J.;
RT "Analysis of the function of two circadian-regulated CONSTANS-LIKE genes.";
RL Plant J. 26:15-22(2001).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12692345; DOI=10.1104/pp.102.016188;
RA Griffiths S., Dunford R.P., Coupland G., Laurie D.A.;
RT "The evolution of CONSTANS-like gene families in barley, rice, and
RT Arabidopsis.";
RL Plant Physiol. 131:1855-1867(2003).
CC -!- FUNCTION: Putative transcription factor. Does not affect flowering
CC time.
CC -!- INTERACTION:
CC Q96502; Q6NLH4: BBX29; NbExp=3; IntAct=EBI-4425260, EBI-15192709;
CC Q96502; Q4PSE2: NFYC8; NbExp=3; IntAct=EBI-4425260, EBI-15191571;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in leaves. Expressed at lower
CC levels in stems, flowers and siliques. Not detected in roots.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development.
CC -!- INDUCTION: Expressed with a circadian rhythm showing a peak at dawn.
CC -!- SIMILARITY: Belongs to the CONSTANS family. {ECO:0000305}.
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DR EMBL; L81120; AAB67880.1; -; mRNA.
DR EMBL; L81119; AAB67879.1; -; Genomic_DNA.
DR EMBL; AC021640; AAF32446.1; -; Genomic_DNA.
DR EMBL; AC068900; AAG12597.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE73800.1; -; Genomic_DNA.
DR EMBL; AF370149; AAK43964.1; -; mRNA.
DR EMBL; AY059092; AAL15198.1; -; mRNA.
DR EMBL; AY088779; AAM67092.1; -; mRNA.
DR RefSeq; NP_186887.1; NM_111105.3.
DR PDB; 7VSP; X-ray; 2.10 A; A/B/C=1-120.
DR PDBsum; 7VSP; -.
DR AlphaFoldDB; Q96502; -.
DR SMR; Q96502; -.
DR BioGRID; 6631; 10.
DR IntAct; Q96502; 6.
DR STRING; 3702.AT3G02380.1; -.
DR PaxDb; Q96502; -.
DR PRIDE; Q96502; -.
DR EnsemblPlants; AT3G02380.1; AT3G02380.1; AT3G02380.
DR GeneID; 821298; -.
DR Gramene; AT3G02380.1; AT3G02380.1; AT3G02380.
DR KEGG; ath:AT3G02380; -.
DR Araport; AT3G02380; -.
DR TAIR; locus:2074587; AT3G02380.
DR eggNOG; KOG1601; Eukaryota.
DR HOGENOM; CLU_028225_3_2_1; -.
DR InParanoid; Q96502; -.
DR OMA; QSCESAP; -.
DR OrthoDB; 1303650at2759; -.
DR PhylomeDB; Q96502; -.
DR PRO; PR:Q96502; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q96502; baseline and differential.
DR Genevisible; Q96502; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR GO; GO:0009909; P:regulation of flower development; IMP:TAIR.
DR InterPro; IPR010402; CCT_domain.
DR InterPro; IPR045281; CONSTANS-like.
DR InterPro; IPR000315; Znf_B-box.
DR PANTHER; PTHR31319; PTHR31319; 1.
DR Pfam; PF06203; CCT; 1.
DR Pfam; PF00643; zf-B_box; 1.
DR SMART; SM00336; BBOX; 2.
DR PROSITE; PS51017; CCT; 1.
DR PROSITE; PS50119; ZF_BBOX; 2.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Metal-binding; Nucleus; Reference proteome;
KW Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..347
FT /note="Zinc finger protein CONSTANS-LIKE 2"
FT /id="PRO_0000113279"
FT DOMAIN 278..320
FT /note="CCT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00357"
FT ZN_FING 16..58
FT /note="B box-type 1; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT ZN_FING 59..101
FT /note="B box-type 2; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 16
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 19
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 39
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 44
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 59
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 62
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 82
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 87
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
SQ SEQUENCE 347 AA; 38524 MW; DDB7B9FB99DF8DF8 CRC64;
MLKEESNESG TWARACDTCR SAACTVYCEA DSAYLCTTCD ARVHAANRVA SRHERVRVCQ
SCESAPAAFL CKADAASLCT ACDAEIHSAN PLARRHQRVP ILPLSANSCS SMAPSETDAD
NDEDDREVAS WLLPNPGKNI GNQNNGFLFG VEYLDLVDYS SSMDNQFEDN QYTHYQRSFG
GDGVVPLQVE ESTSHLQQSQ QNFQLGINYG FSSGAHYNNN SLKDLNHSAS VSSMDISVVP
ESTASDITVQ HPRTTKETID QLSGPPTQVV QQLTPMEREA RVLRYREKKK TRKFDKTIRY
ASRKAYAEIR PRIKGRFAKR IETEAEAEEI FSTSLMSETG YGIVPSF
