COLD1_ORYSJ
ID COLD1_ORYSJ Reviewed; 468 AA.
AC Q7X7S8; A0A0P0WEQ4; A3AX31; Q0JAG1;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=GPCR-type G protein COLD1 {ECO:0000305};
DE AltName: Full=Protein CHILLING TOLERANCE DIVERGENCE 1 {ECO:0000303|PubMed:25728666};
GN Name=COLD1 {ECO:0000303|PubMed:25728666};
GN OrderedLocusNames=Os04g0600800 {ECO:0000312|EMBL:BAF15676.2},
GN LOC_Os04g51180 {ECO:0000305};
GN ORFNames=OsJ_16035 {ECO:0000312|EMBL:EAZ31870.1},
GN OSJNBa0083N12.15 {ECO:0000312|EMBL:CAD41818.2};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RG The rice full-length cDNA consortium;
RT "Oryza sativa full length cDNA.";
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION, INTERACTION WITH GPA1/RGA1, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, CLEAVAGE OF INITIATOR METHIONINE, MYRISTOYLATION AT GLY-2, AND
RP MUTAGENESIS OF GLY-2.
RC STRAIN=cv. Dongjin, and cv. Nipponbare;
RX PubMed=25728666; DOI=10.1016/j.cell.2015.01.046;
RA Ma Y., Dai X., Xu Y., Luo W., Zheng X., Zeng D., Pan Y., Lin X., Liu H.,
RA Zhang D., Xiao J., Guo X., Xu S., Niu Y., Jin J., Zhang H., Xu X., Li L.,
RA Wang W., Qian Q., Ge S., Chong K.;
RT "COLD1 confers chilling tolerance in rice.";
RL Cell 160:1209-1221(2015).
CC -!- FUNCTION: Involved in chilling tolerance. Interacts with the G-protein
CC alpha subunit GPA1 to activate the calcium channel for sensing low
CC temperature and to accelerate G-protein GTPase activity.
CC {ECO:0000269|PubMed:25728666}.
CC -!- SUBUNIT: Interacts with GPA1/RGA1. {ECO:0000269|PubMed:25728666}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:25728666};
CC Multi-pass membrane protein {ECO:0000255}. Endoplasmic reticulum
CC membrane {ECO:0000269|PubMed:25728666}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants are sensitive to cold.
CC {ECO:0000269|PubMed:25728666}.
CC -!- MISCELLANEOUS: In cold sensitive indica cultivars (AC A2XX57) Lys-187
CC is replaced by Met-187 or Thr-187. These polymorphisms are associated
CC with divergence in chilling tolerance of rice cultivars. COLD1 confers
CC adaptation of japonica rice to chilling and originated from the Chinese
CC wild populations of Oryza rufipogon. {ECO:0000269|PubMed:25728666}.
CC -!- SIMILARITY: Belongs to the Golgi pH regulator (TC 1.A.38) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF15676.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL606683; CAD41818.2; -; Genomic_DNA.
DR EMBL; AP008210; BAF15676.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014960; BAS90836.1; -; Genomic_DNA.
DR EMBL; CM000141; EAZ31870.1; -; Genomic_DNA.
DR EMBL; AK318606; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015633398.1; XM_015777912.1.
DR AlphaFoldDB; Q7X7S8; -.
DR STRING; 4530.OS04T0600800-01; -.
DR iPTMnet; Q7X7S8; -.
DR PaxDb; Q7X7S8; -.
DR PRIDE; Q7X7S8; -.
DR EnsemblPlants; Os04t0600800-01; Os04t0600800-01; Os04g0600800.
DR GeneID; 4336878; -.
DR Gramene; Os04t0600800-01; Os04t0600800-01; Os04g0600800.
DR KEGG; osa:4336878; -.
DR eggNOG; KOG2417; Eukaryota.
DR HOGENOM; CLU_030540_1_0_1; -.
DR InParanoid; Q7X7S8; -.
DR OMA; HFNFYHR; -.
DR OrthoDB; 1065554at2759; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000007752; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR Genevisible; Q7X7S8; OS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0010427; F:abscisic acid binding; IBA:GO_Central.
DR GO; GO:0051020; F:GTPase binding; IPI:UniProtKB.
DR GO; GO:0070417; P:cellular response to cold; IMP:UniProtKB.
DR GO; GO:0009737; P:response to abscisic acid; IBA:GO_Central.
DR InterPro; IPR025969; ABA_GPCR_dom.
DR InterPro; IPR022535; Golgi_pH-regulator_cons_dom.
DR InterPro; IPR015672; GPHR/GTG.
DR PANTHER; PTHR15948; PTHR15948; 1.
DR Pfam; PF12430; ABA_GPCR; 1.
DR Pfam; PF12537; GPHR_N; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Coiled coil; Endoplasmic reticulum; Lipoprotein; Membrane;
KW Myristate; Reference proteome; Stress response; Transmembrane;
KW Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000305|PubMed:25728666"
FT CHAIN 2..468
FT /note="GPCR-type G protein COLD1"
FT /id="PRO_0000432809"
FT TRANSMEM 7..27
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..319
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 345..365
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..457
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT COILED 243..279
FT /evidence="ECO:0000255"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000305|PubMed:25728666"
FT MUTAGEN 2
FT /note="G->A: No effect on cellular localization."
FT /evidence="ECO:0000269|PubMed:25728666"
FT CONFLICT 9
FT /note="G -> E (in Ref. 6; AK318606 and 5; EAZ31870)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 468 AA; 53332 MW; 5205D49B2E0530D3 CRC64;
MGWGAVVYGG GVVGASLVGL GWAGLWFLNR RLYKEYEERR ALVQILFGLV FAFSCNLFQL
VLFEILPVLS KHARFLNWHL DLFCLILLLV FVLPYYHCYL LLRNSGVRRE RALLVAALFL
LVFLYGFWRM GIHFPMPSPE KGFFTMPQLV SRIGVIGVSV MAVLSGFGAV NLPYSYLSLF
IREIDEKDIK TLERQLMQSM ETCIAKKKKI VLSKMEMERI QGSEEKLKAR SFLKRIVGTV
VRSVQEDQTE QDIKSLDAEV QALEELSKQL FLEIYELRQA KIAAAFSRTW RGHAQNLLGY
ALSVYCVYKM LKSLQSVVFK EAGSVDPVTM TITIFLRHFD IGIDVTLLSQ YISLIFIGML
VVISVRGFLA NVMKFFFAVS RVGSGSTTNV VLFLSEIMGM YFISSILLIR KSLANEYRVI
ITDVLGGDIQ FDFYHRWFDA IFVASAFLSL LLISAQYTSR QTDKHPID
