COLT_DROME
ID COLT_DROME Reviewed; 306 AA.
AC Q9VQG4; A4V020; O01396;
DT 15-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Congested-like trachea protein;
GN Name=colt; ORFNames=CG3057;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=Oregon-R;
RX PubMed=9409834; DOI=10.1093/genetics/147.4.1755;
RA Hartenstein K., Sinha P., Mishra A., Schenkel H., Toeroek I., Mechler B.M.;
RT "The congested-like tracheae gene of Drosophila melanogaster encodes a
RT member of the mitochondrial carrier family required for gas-filling of the
RT tracheal system and expansion of the wings after eclosion.";
RL Genetics 147:1755-1768(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Ovary;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Putative mitochondrial carrier of unknown solute specificity.
CC Required for gas-filling of the tracheal system at hatching time of the
CC embryo and for normal epithelial morphogenesis of the wings.
CC {ECO:0000269|PubMed:9409834}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically during
CC all developmental stages. {ECO:0000269|PubMed:9409834}.
CC -!- DISRUPTION PHENOTYPE: Flies display defects in the unfolding and
CC expansion of the wings resulting in a loss of venation and a marked
CC decrease in their size. Lethality is polyphasic with flies dying during
CC early larval development and displaying apparently collapsed tracheal
CC trees. {ECO:0000269|PubMed:9409834}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Y12495; CAA73099.1; -; Genomic_DNA.
DR EMBL; AE014134; AAF51209.1; -; Genomic_DNA.
DR EMBL; AE014134; AAS64622.1; -; Genomic_DNA.
DR EMBL; AE014134; AAS64623.1; -; Genomic_DNA.
DR EMBL; AE014134; AAS64624.1; -; Genomic_DNA.
DR EMBL; AY069277; AAL39422.1; -; mRNA.
DR RefSeq; NP_477221.1; NM_057873.5.
DR RefSeq; NP_995616.1; NM_205894.1.
DR AlphaFoldDB; Q9VQG4; -.
DR SMR; Q9VQG4; -.
DR BioGRID; 59693; 3.
DR STRING; 7227.FBpp0077414; -.
DR PaxDb; Q9VQG4; -.
DR PRIDE; Q9VQG4; -.
DR DNASU; 33470; -.
DR EnsemblMetazoa; FBtr0077731; FBpp0077414; FBgn0019830.
DR EnsemblMetazoa; FBtr0077734; FBpp0089377; FBgn0019830.
DR GeneID; 33470; -.
DR KEGG; dme:Dmel_CG3057; -.
DR CTD; 33470; -.
DR FlyBase; FBgn0019830; colt.
DR VEuPathDB; VectorBase:FBgn0019830; -.
DR eggNOG; KOG0758; Eukaryota.
DR GeneTree; ENSGT00940000167609; -.
DR HOGENOM; CLU_015166_16_0_1; -.
DR InParanoid; Q9VQG4; -.
DR OMA; QYTIGQI; -.
DR OrthoDB; 1072378at2759; -.
DR PhylomeDB; Q9VQG4; -.
DR Reactome; R-DME-200425; Carnitine metabolism.
DR BioGRID-ORCS; 33470; 0 hits in 3 CRISPR screens.
DR ChiTaRS; colt; fly.
DR GenomeRNAi; 33470; -.
DR PRO; PR:Q9VQG4; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0019830; Expressed in Malpighian tubule and 14 other tissues.
DR Genevisible; Q9VQG4; DM.
DR GO; GO:0016021; C:integral component of membrane; ISM:FlyBase.
DR GO; GO:0005740; C:mitochondrial envelope; IBA:GO_Central.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015227; F:acyl carnitine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:1902603; P:carnitine transmembrane transport; IBA:GO_Central.
DR GO; GO:0003382; P:epithelial cell morphogenesis; IMP:FlyBase.
DR GO; GO:0035002; P:liquid clearance, open tracheal system; IMP:FlyBase.
DR GO; GO:0006839; P:mitochondrial transport; IBA:GO_Central.
DR Gene3D; 1.50.40.10; -; 2.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Developmental protein; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..306
FT /note="Congested-like trachea protein"
FT /id="PRO_0000090608"
FT TRANSMEM 19..39
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..196
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 13..104
FT /note="Solcar 1"
FT REPEAT 113..202
FT /note="Solcar 2"
FT REPEAT 212..298
FT /note="Solcar 3"
FT CONFLICT 96
FT /note="A -> T (in Ref. 1; CAA73099)"
FT /evidence="ECO:0000305"
FT CONFLICT 186..187
FT /note="LP -> FA (in Ref. 1; CAA73099)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 306 AA; 32952 MW; FA66FF1DA600F5F9 CRC64;
MTTTENVSTE RKANPVKSFL TGGFGGICNV LSGHPLDTIK VRLQTMPRPA PGEQPLYRGT
FDCAAKTIKN EGVRGLYKGM SAPLTGVAPI FAMCFAGYAL GKRLQQRGED AKLTYPQIFV
AGSFSGLFST LIMAPGERIK VLLQTQQGQG GERKYNGMID CAGKLYKEGG LRSVFKGSCA
TMLRDLPANG LYFLVYEALQ DVAKSKSETG QISTASTIFA GGVAGMAYWI LGMPADVLKS
RLQSAPEGTY KHGIRSVFKD LIVKDGPLAL YRGVTPIMLR AFPANAACFF GIELANKFFN
IVAPNF
