ID   COMB_STRPN              Reviewed;         449 AA.
AC   P36498;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   26-SEP-2001, sequence version 4.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Transport protein ComB;
GN   Name=comB; OrderedLocusNames=SP_0043;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Rx / CP1200;
RX   PubMed=7883181; DOI=10.1016/0378-1119(94)00841-f;
RA   Hui F.M., Zhou L., Morrison D.A.;
RT   "Competence for genetic transformation in Streptococcus pneumoniae:
RT   organization of a regulatory locus with homology to two lactococcin A
RT   secretion genes.";
RL   Gene 153:25-31(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA   Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA   Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-107.
RC   STRAIN=Rx / CP1200;
RX   PubMed=1987129; DOI=10.1128/jb.173.1.372-381.1991;
RA   Hui F.M., Morrison D.A.;
RT   "Genetic transformation in Streptococcus pneumoniae: nucleotide sequence
RT   analysis shows comA, a gene required for competence induction, to be a
RT   member of the bacterial ATP-dependent transport protein family.";
RL   J. Bacteriol. 173:372-381(1991).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 400-449.
RC   STRAIN=Rx / CP1200;
RX   PubMed=8407811; DOI=10.1128/jb.175.19.6364-6367.1993;
RA   Hui F.M., Morrison D.A.;
RT   "Identification of a purC gene from Streptococcus pneumoniae.";
RL   J. Bacteriol. 175:6364-6367(1993).
CC   -!- FUNCTION: Required for induction of competence.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; M36180; AAA69511.1; -; Genomic_DNA.
DR   EMBL; AE005672; AAK74232.1; -; Genomic_DNA.
DR   EMBL; L15190; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; B36941; B36941.
DR   PIR; B39203; B39203.
DR   PIR; D97877; D97877.
DR   PIR; G95004; G95004.
DR   RefSeq; WP_000801611.1; NZ_AKVY01000001.1.
DR   AlphaFoldDB; P36498; -.
DR   SMR; P36498; -.
DR   STRING; 170187.SP_0043; -.
DR   TCDB; 8.A.1.4.2; the membrane fusion protein (mfp) family.
DR   EnsemblBacteria; AAK74232; AAK74232; SP_0043.
DR   KEGG; spn:SP_0043; -.
DR   eggNOG; COG0845; Bacteria.
DR   OMA; HINDEYS; -.
DR   PhylomeDB; P36498; -.
DR   BioCyc; SPNE170187:G1FZB-48-MON; -.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR   InterPro; IPR005696; MesE/LcnD.
DR   TIGRFAMs; TIGR01000; bacteriocin_acc; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Competence; Membrane; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..449
FT                   /note="Transport protein ComB"
FT                   /id="PRO_0000201864"
FT   TOPO_DOM        1..20
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        21..41
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        42..449
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        202
FT                   /note="A -> T (in Ref. 1; AAA69511)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        286
FT                   /note="T -> S (in Ref. 1; AAA69511)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        311
FT                   /note="V -> I (in Ref. 1; AAA69511)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        335
FT                   /note="A -> T (in Ref. 1; AAA69511)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        360
FT                   /note="Y -> D (in Ref. 1; AAA69511)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   449 AA;  49601 MW;  8798BEF7A2E82310 CRC64;
     MKPEFLESAE FYNRRYHNFS SSVIVPMALL LVFLLGFATV AEKEMSLSTR ATVEPSRILA
     NIQSTSNNRI LVNHLEENKL VKKGDLLVQY QEGAEGVQAE SYASQLDMLK DQKKQLEYLQ
     KSLQEGENHF PEEDKFGYQA TFRDYISQAG SLRASTSQQN ETIASQNAAA SQTQAEIGNL
     ISQTEAKIRD YQTAKSAIET GASLAGQNLA YSLYQSYKSQ GEENPQTKVQ AVAQVEAQIS
     QLESSLATYR VQYAGSGTQQ AYASGLSSQL ESLKSQHLAK VGQELTLLAQ KILEAESGKK
     VQGNLLDKGK VTASEDGVLH LNPETSDSSM VAEGALLAQL YPSLEREGKA KLTAYLSSKY
     VARIKVGDSV RYTTTHDAGN QLFLDSTITS IDATATKTEK GNFFKIEAET NLTSEQAEKL
     RYGVEGRLQM ITGKKSYLRY YLDQFLNKE