ID   COMB_THEMA              Reviewed;         227 AA.
AC   Q9WZQ4;
DT   05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Probable 2-phosphosulfolactate phosphatase;
DE            EC=3.1.3.71;
GN   Name=comB; OrderedLocusNames=TM_0797;
OS   Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS   / MSB8).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=243274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=10360571; DOI=10.1038/20601;
RA   Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA   Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA   Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA   Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA   Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA   Smith H.O., Venter J.C., Fraser C.M.;
RT   "Evidence for lateral gene transfer between Archaea and Bacteria from
RT   genome sequence of Thermotoga maritima.";
RL   Nature 399:323-329(1999).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R)-O-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate +
CC         phosphate; Xref=Rhea:RHEA:23416, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15597, ChEBI:CHEBI:43474, ChEBI:CHEBI:58738; EC=3.1.3.71;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the ComB family. {ECO:0000305}.
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DR   EMBL; AE000512; AAD35879.1; -; Genomic_DNA.
DR   PIR; F72334; F72334.
DR   RefSeq; NP_228606.1; NC_000853.1.
DR   RefSeq; WP_004080870.1; NZ_CP011107.1.
DR   PDB; 2YYV; X-ray; 1.65 A; A/B=1-227.
DR   PDB; 2YZO; X-ray; 1.85 A; A/B=1-227.
DR   PDBsum; 2YYV; -.
DR   PDBsum; 2YZO; -.
DR   AlphaFoldDB; Q9WZQ4; -.
DR   SMR; Q9WZQ4; -.
DR   STRING; 243274.THEMA_00665; -.
DR   EnsemblBacteria; AAD35879; AAD35879; TM_0797.
DR   KEGG; tma:TM0797; -.
DR   eggNOG; COG2045; Bacteria.
DR   InParanoid; Q9WZQ4; -.
DR   OMA; RLFMSTT; -.
DR   OrthoDB; 1617556at2; -.
DR   EvolutionaryTrace; Q9WZQ4; -.
DR   Proteomes; UP000008183; Chromosome.
DR   GO; GO:0050532; F:2-phosphosulfolactate phosphatase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0050545; F:sulfopyruvate decarboxylase activity; IBA:GO_Central.
DR   Gene3D; 3.90.1560.10; -; 1.
DR   HAMAP; MF_00490; ComB; 1.
DR   InterPro; IPR005238; ComB-like.
DR   InterPro; IPR036702; ComB-like_sf.
DR   PANTHER; PTHR37311; PTHR37311; 1.
DR   Pfam; PF04029; 2-ph_phosp; 1.
DR   SUPFAM; SSF142823; SSF142823; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase; Magnesium; Reference proteome.
FT   CHAIN           1..227
FT                   /note="Probable 2-phosphosulfolactate phosphatase"
FT                   /id="PRO_0000081477"
FT   STRAND          3..7
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   STRAND          15..20
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   TURN            22..24
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   HELIX           25..34
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   STRAND          38..42
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   HELIX           46..51
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   STRAND          57..61
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   HELIX           77..79
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   HELIX           82..85
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   STRAND          89..93
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   HELIX           97..103
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   STRAND          109..112
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   HELIX           114..116
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   HELIX           117..124
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   STRAND          129..134
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   HELIX           143..156
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   HELIX           163..173
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   HELIX           178..185
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   HELIX           187..194
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   HELIX           198..204
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   STRAND          215..217
FT                   /evidence="ECO:0007829|PDB:2YYV"
FT   STRAND          220..222
FT                   /evidence="ECO:0007829|PDB:2YYV"
SQ   SEQUENCE   227 AA;  24857 MW;  ED32447E6140D9F8 CRC64;
     MVDVVMAPCS PVECRTAVVI DVLRATSTIV TALSNGASGV IPVKTIEEAL EKKKEGVLIC
     GERNAQKPKG FNLGNSPLEY RKEKISGKTI VLTTTNGTQV IEKIRSEEII AASFLNLSAV
     VEYLKSKEDI LLVCAGTNGR FSLEDFLLAG AIVKRLKRND LGDGAHAAER YFESVENTRE
     EIKKHSSHAK RLISLGFEND IEFCTTEDLF KTVPALVNGV FILKEFP