ID   COMF_DICDI              Reviewed;        1106 AA.
AC   Q557B8; Q86MX4;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Communication mutant protein F;
DE   Flags: Precursor;
GN   Name=comF-1; ORFNames=DDB_G0273257;
GN   and
GN   Name=comF-2; ORFNames=DDB_G0273669;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 252-354, AND DISRUPTION PHENOTYPE.
RX   PubMed=12871696; DOI=10.1016/s0012-1606(03)00204-5;
RA   Kibler K., Nguyen T.-L., Svetz J., Van Driessche N., Ibarra M.,
RA   Thompson C., Shaw C., Shaulsky G.;
RT   "A novel developmental mechanism in Dictyostelium revealed in a screen for
RT   communication mutants.";
RL   Dev. Biol. 259:193-208(2003).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Shows no aggregation.
CC       {ECO:0000269|PubMed:12871696}.
CC   -!- SIMILARITY: Belongs to the comF family. {ECO:0000305}.
CC   -!- CAUTION: The gene for this protein is duplicated in strains AX3 and
CC       AX4. These strains contain a duplication of a segment of 750 kb of
CC       chromosome 2 compared to the corresponding sequence in strain AX2.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000011; EAL70525.1; -; Genomic_DNA.
DR   EMBL; AAFI02000009; EAL70846.1; -; Genomic_DNA.
DR   EMBL; AY221643; AAO34399.1; -; Genomic_DNA.
DR   RefSeq; XP_644451.1; XM_639359.1.
DR   RefSeq; XP_644799.1; XM_639707.1.
DR   AlphaFoldDB; Q557B8; -.
DR   PaxDb; Q557B8; -.
DR   PRIDE; Q557B8; -.
DR   EnsemblProtists; EAL70525; EAL70525; DDB_G0273669.
DR   EnsemblProtists; EAL70846; EAL70846; DDB_G0273257.
DR   GeneID; 8618901; -.
DR   GeneID; 8619076; -.
DR   KEGG; ddi:DDB_G0273257; -.
DR   KEGG; ddi:DDB_G0273669; -.
DR   dictyBase; DDB_G0273257; comF-1.
DR   dictyBase; DDB_G0273669; comF-2.
DR   HOGENOM; CLU_005143_0_0_1; -.
DR   InParanoid; Q557B8; -.
DR   OMA; HIHANCA; -.
DR   PhylomeDB; Q557B8; -.
DR   PRO; PR:Q557B8; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR   InterPro; IPR019316; G8_domain.
DR   Pfam; PF10162; G8; 1.
DR   SMART; SM01225; G8; 1.
DR   PROSITE; PS51484; G8; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..1106
FT                   /note="Communication mutant protein F"
FT                   /id="PRO_0000393589"
FT   DOMAIN          254..380
FT                   /note="G8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00817"
FT   CARBOHYD        267
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        306
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        512
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        536
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        677
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        715
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        833
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1106 AA;  124031 MW;  A4DD3B581F08FC74 CRC64;
     MKIYKKNHFL KILIIFIYLS CNILKVNADG TFSTIQPSTD IKFDKSNLEF VAEWDVSANS
     ISNVVGSTDS SEFSLNYGVW GPTYNYLIAK NKSPSIKANV DYTFSFDFKL GKALGEYNNY
     QSMVLSFYIP EDIAYFPWEI RTPLYTTNEF SGNFASTSYQ SKILTFRSTV DIGNSIMVLR
     INRATETGPT ASSVYFRNMK ITIPSKPITT PTDLLTKDSE LIVIPKPPIS LDLQDLTKCP
     YLTASDLYNW HDPTIWPNGV VPSPNQNITI PAGKRVLISA SSISQTQIYS RIVVPVNSEL
     IFNDQNFTMN IRDIYVQGKF IMGTSLCRYN SFINIIFHGE KTLQDTIAQF YGSKGIAVAS
     GGFISVQGKQ YHNTWSKLAS NVWSGDRVIW IQDNVNWEVG QQVLITTSVY KDELDNQNEI
     LTIKAIEGKK IEFTEPIKWF KYGSQEYQSE VALLSRRIVF SSDESSSVST SFGGHILSSG
     EMQFAGVQLK RMGQKNVKAR YPLHYHLGGT LNNSFISDCS VTNSYYRCYT IHGTNNVTLT
     RNVAYDAFGH CYYLEDGVEV DNRISFNLGA YVHTIGKPAA GASQIGETFY QSSELTQPAD
     SAASCFYITN SWNSFIGNAA SGGWAGFAFP NLPKPIGNHR TLNIIPMQYP IKEWQGNTAH
     SSGYYFEDGA SIYVGGNLTF NEANGMLIYN SGRLGRTTYV NGVKNENNVV FDRLNNTKIF
     LSNLGVGYWG ENIEIVGYES HDNTRPVSLF GNVWLHNALV NGQSGNILTK NSETTRQGFR
     FYDFYVQTII SNTIFRNFIH STAATKRDED NVVITATTFS DVFKPQFISA TKNITFQNVP
     QSQIIGHEDV ANSGSSRLFN FLDGDGSVTS SFTGKPGIPQ IVGSHVSWWK FDDSCLFSTE
     WNVWVCNKGT KGLANIEFWV PGFMERELEQ DPDSYIGSIS LFGSGINDER KTLLTRNPGI
     TGVSNMGWYA YFTIGTPNYL RIWTAQIAFG EYIFLAIPYP TGTTFTITSE YDYSNQYTYT
     ITKTTSALAV KQGNGKQYYF DGTHLFLKLV NFMNTGGSWE SFDRVGIKIP DVYWTYIYNI
     RATNTAKPSV NGYFLNLPDV RPSSTL