ID   COMGB_BACSU             Reviewed;         323 AA.
AC   P25954;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=ComG operon protein 2;
GN   Name=comGB; Synonyms=comG2; OrderedLocusNames=BSU24720;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2507524; DOI=10.1128/jb.171.10.5386-5404.1989;
RA   Albano M., Breitling R., Dubnau D.A.;
RT   "Nucleotide sequence and genetic organization of the Bacillus subtilis comG
RT   operon.";
RL   J. Bacteriol. 171:5386-5404(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   FUNCTION.
RX   PubMed=9422590; DOI=10.1128/jb.180.1.41-45.1998;
RA   Chung Y.S., Dubnau D.A.;
RT   "All seven comG open reading frames are required for DNA binding during
RT   transformation of competent Bacillus subtilis.";
RL   J. Bacteriol. 180:41-45(1998).
CC   -!- FUNCTION: Required for transformation and DNA binding.
CC       {ECO:0000269|PubMed:9422590}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the GSP F family. {ECO:0000305}.
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DR   EMBL; M29691; AAA83368.1; -; Genomic_DNA.
DR   EMBL; D84432; BAA12534.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14403.1; -; Genomic_DNA.
DR   PIR; C30338; C30338.
DR   RefSeq; NP_390352.1; NC_000964.3.
DR   RefSeq; WP_003246116.1; NC_000964.3.
DR   AlphaFoldDB; P25954; -.
DR   SMR; P25954; -.
DR   STRING; 224308.BSU24720; -.
DR   TCDB; 3.A.14.1.1; the fimbrilin/protein exporter (fpe) family.
DR   PaxDb; P25954; -.
DR   PRIDE; P25954; -.
DR   EnsemblBacteria; CAB14403; CAB14403; BSU_24720.
DR   GeneID; 938527; -.
DR   KEGG; bsu:BSU24720; -.
DR   PATRIC; fig|224308.43.peg.2579; -.
DR   eggNOG; COG1459; Bacteria.
DR   InParanoid; P25954; -.
DR   OMA; TRQMATM; -.
DR   PhylomeDB; P25954; -.
DR   BioCyc; BSUB:BSU24720-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   Gene3D; 1.20.81.30; -; 2.
DR   InterPro; IPR003004; GspF/PilC.
DR   InterPro; IPR001992; T2SS_GspF/T4SS_PilC_CS.
DR   InterPro; IPR018076; T2SS_GspF_dom.
DR   InterPro; IPR042094; T2SS_GspF_sf.
DR   PANTHER; PTHR30012; PTHR30012; 1.
DR   Pfam; PF00482; T2SSF; 2.
DR   PROSITE; PS00874; T2SP_F; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Competence; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..323
FT                   /note="ComG operon protein 2"
FT                   /id="PRO_0000207839"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        296..316
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   323 AA;  37057 MW;  49F371B18811A1CE CRC64;
     MTAGGYTLLD GLRLMELQMN KRQAADLTDS VTCLREGAPF YQVLKSLSFH KEAVGICYFA
     ETHGELPASM IQSGELLERK IAQADQLKRV LRYPLFLIFT VAVMFYMLQS IIIPQFSGIY
     QSMNMETSRS TDMLFAFFQH IDLVIILLVL FTAGIGIYYW LVFKKKSPAR QMLICIRIPL
     VGKLVKLFNS YFFSLQLSSL LKSGLSIYDS LNAFKHQTFL PFYRCEAEQL IERLKAGESI
     ESAICGSLFY ETDLSKVISH GQLSGRLDRE LFTYSQFILQ RLEHKAQKWT GILQPMIYGF
     VAAMILLVYL SMLVPMYQMM NQM