ID   COMGE_BACSU             Reviewed;         115 AA.
AC   P25957;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   04-AUG-2003, sequence version 3.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=ComG operon protein 5;
DE   Flags: Precursor;
GN   Name=comGE; Synonyms=comG5; OrderedLocusNames=BSU24690;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2507524; DOI=10.1128/jb.171.10.5386-5404.1989;
RA   Albano M., Breitling R., Dubnau D.A.;
RT   "Nucleotide sequence and genetic organization of the Bacillus subtilis comG
RT   operon.";
RL   J. Bacteriol. 171:5386-5404(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   FUNCTION.
RX   PubMed=9422590; DOI=10.1128/jb.180.1.41-45.1998;
RA   Chung Y.S., Dubnau D.A.;
RT   "All seven comG open reading frames are required for DNA binding during
RT   transformation of competent Bacillus subtilis.";
RL   J. Bacteriol. 180:41-45(1998).
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   PubMed=9723928; DOI=10.1046/j.1365-2958.1998.00989.x;
RA   Chung Y.S., Breidt F., Dubnau D.A.;
RT   "Cell surface localization and processing of the ComG proteins, required
RT   for DNA binding during transformation of Bacillus subtilis.";
RL   Mol. Microbiol. 29:905-913(1998).
CC   -!- FUNCTION: Required for transformation and DNA binding.
CC       {ECO:0000269|PubMed:9422590}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:9723928};
CC       Single-pass membrane protein {ECO:0000269|PubMed:9723928}. Cell surface
CC       {ECO:0000269|PubMed:9723928}. Note=The unprocessed form is an integral
CC       membrane protein. Upon cleavage, it is translocated to the outer face
CC       of the membrane.
CC   -!- PTM: Processing of ComGE in competent cells requires ComC.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M29691; AAA83371.1; -; Genomic_DNA.
DR   EMBL; D84432; BAA12537.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14400.1; -; Genomic_DNA.
DR   PIR; F30338; F30338.
DR   RefSeq; NP_390349.1; NC_000964.3.
DR   RefSeq; WP_003230165.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; P25957; -.
DR   SMR; P25957; -.
DR   STRING; 224308.BSU24690; -.
DR   PaxDb; P25957; -.
DR   PRIDE; P25957; -.
DR   DNASU; 938518; -.
DR   EnsemblBacteria; CAB14400; CAB14400; BSU_24690.
DR   GeneID; 938518; -.
DR   KEGG; bsu:BSU24690; -.
DR   PATRIC; fig|224308.179.peg.2687; -.
DR   eggNOG; ENOG5030P9X; Bacteria.
DR   OMA; MWRENKG; -.
DR   BioCyc; BSUB:BSU24690-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR   InterPro; IPR012902; N_methyl_site.
DR   PROSITE; PS00409; PROKAR_NTER_METHYL; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Competence; Membrane; Methylation; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   PROPEP          1..7
FT                   /note="Leader sequence"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01070"
FT                   /id="PRO_0000024270"
FT   CHAIN           8..115
FT                   /note="ComG operon protein 5"
FT                   /id="PRO_0000024271"
FT   TRANSMEM        13..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         8
FT                   /note="N-methylphenylalanine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01070"
FT   CONFLICT        88
FT                   /note="Q -> E (in Ref. 1; AAA83371)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   115 AA;  13271 MW;  0A85FD247285E9D2 CRC64;
     MWRENKGFST IETMSALSLW LFVLLTVVPL WDKLMADEKM AESREIGYQM MNESISKYVM
     SGEGAASKTI TKNNHIYAMK WEEEGEYQNV CIKAAAYKEK SFCLSILQTE WLHAS