ID   COMK_BACSU              Reviewed;         192 AA.
AC   P40396;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Competence transcription factor;
DE            Short=CTF;
DE   AltName: Full=Competence protein K;
GN   Name=comK; OrderedLocusNames=BSU10420;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=8196543; DOI=10.1111/j.1365-2958.1994.tb00347.x;
RA   van Sinderen D., ten Berge A., Hayema B.J., Hamoen L., Venema G.;
RT   "Molecular cloning and sequence of comK, a gene required for genetic
RT   competence in Bacillus subtilis.";
RL   Mol. Microbiol. 11:695-703(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9579061; DOI=10.1099/00221287-144-4-859;
RA   Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H.,
RA   Venema G., Bron S.;
RT   "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus
RT   subtilis chromosome contains several dysfunctional genes, the glyB marker,
RT   many genes encoding transporter proteins, and the ubiquitous hit gene.";
RL   Microbiology 144:859-875(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   FUNCTION, INDUCTION, AND DNA-BINDING.
RX   PubMed=7783616; DOI=10.1111/j.1365-2958.1995.tb02259.x;
RA   van Sinderen D., Luttinger A., Kong L., Dubnau D., Venema G., Hamoen L.;
RT   "comK encodes the competence transcription factor, the key regulatory
RT   protein for competence development in Bacillus subtilis.";
RL   Mol. Microbiol. 15:455-462(1995).
RN   [5]
RP   REGULATION OF THE BDBDC OPERON.
RC   STRAIN=168;
RX   PubMed=11744713; DOI=10.1074/jbc.m111380200;
RA   Meima R., Eschevins C., Fillinger S., Bolhuis A., Hamoen L.W., Dorenbos R.,
RA   Quax W.J., van Dijl J.M., Provvedi R., Chen I., Dubnau D., Bron S.;
RT   "The bdbDC operon of Bacillus subtilis encodes thiol-disulfide
RT   oxidoreductases required for competence development.";
RL   J. Biol. Chem. 277:6994-7001(2002).
RN   [6]
RP   FUNCTION, INDUCTION, AND DNA-BINDING.
RC   STRAIN=BD630;
RX   PubMed=11849533; DOI=10.1046/j.1365-2958.2002.02727.x;
RA   Hoa T.T., Tortosa P., Albano M., Dubnau D.;
RT   "Rok (YkuW) regulates genetic competence in Bacillus subtilis by directly
RT   repressing comK.";
RL   Mol. Microbiol. 43:15-26(2002).
RN   [7]
RP   FUNCTION, REGULON, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168;
RX   PubMed=11918817; DOI=10.1046/j.1365-2958.2002.02833.x;
RA   Berka R.M., Hahn J., Albano M., Draskovic I., Persuh M., Cui X., Sloma A.,
RA   Widner W., Dubnau D.;
RT   "Microarray analysis of the Bacillus subtilis K-state: genome-wide
RT   expression changes dependent on ComK.";
RL   Mol. Microbiol. 43:1331-1345(2002).
RN   [8]
RP   FUNCTION, REGULON, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168 / CU741;
RX   PubMed=11948146; DOI=10.1128/jb.184.9.2344-2351.2002;
RA   Ogura M., Yamaguchi H., Kobayashi K., Ogasawara N., Fujita Y., Tanaka T.;
RT   "Whole-genome analysis of genes regulated by the Bacillus subtilis
RT   competence transcription factor ComK.";
RL   J. Bacteriol. 184:2344-2351(2002).
CC   -!- FUNCTION: A master regulator required for the expression of late
CC       competence genes including comC, comE, comG and the bdbDC operon
CC       (PubMed:8196543, PubMed:7783616, PubMed:11744713, PubMed:11918817,
CC       PubMed:11948146). Receives signals from SrfA, and possibly other
CC       regulatory COM genes, and transduces these signals to the late COM
CC       genes (PubMed:8196543, PubMed:7783616). Represses transcription of rok
CC       (PubMed:11849533). May repress expression of a few genes
CC       (PubMed:7783616). {ECO:0000269|PubMed:11744713,
CC       ECO:0000269|PubMed:11849533, ECO:0000269|PubMed:11918817,
CC       ECO:0000269|PubMed:11948146, ECO:0000269|PubMed:7783616,
CC       ECO:0000269|PubMed:8196543}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in cells competent for DNA
CC       transformation; that is 5-10% of the population (PubMed:11918817).
CC       {ECO:0000269|PubMed:11918817}.
CC   -!- INDUCTION: Positively regulates its own expression (PubMed:7783616).
CC       Expressed only in glucose-based minimal medium during the transition to
CC       stationary phase (PubMed:8196543). Expression is initiated at the time
CC       of transition to the post-exponential state, and exclusively in
CC       competence-stimulating medium. Repressed by AbrB and Rok
CC       (PubMed:11849533). {ECO:0000269|PubMed:11849533,
CC       ECO:0000269|PubMed:7783616, ECO:0000269|PubMed:8196543}.
CC   -!- DISRUPTION PHENOTYPE: Transformation deficient (PubMed:8196543). Loss
CC       of expression of at least 165 genes (PubMed:11918817, PubMed:11948146).
CC       {ECO:0000269|PubMed:11918817, ECO:0000269|PubMed:11948146,
CC       ECO:0000269|PubMed:8196543}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA74548.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; S70734; AAB30864.1; -; Genomic_DNA.
DR   EMBL; Y14084; CAA74548.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AL009126; CAB12882.1; -; Genomic_DNA.
DR   PIR; S43611; S43611.
DR   RefSeq; NP_388923.1; NC_000964.3.
DR   RefSeq; WP_003245329.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; P40396; -.
DR   SMR; P40396; -.
DR   IntAct; P40396; 1.
DR   MINT; P40396; -.
DR   STRING; 224308.BSU10420; -.
DR   PaxDb; P40396; -.
DR   PRIDE; P40396; -.
DR   EnsemblBacteria; CAB12882; CAB12882; BSU_10420.
DR   GeneID; 936338; -.
DR   KEGG; bsu:BSU10420; -.
DR   PATRIC; fig|224308.179.peg.1120; -.
DR   eggNOG; COG4903; Bacteria.
DR   OMA; GITHKAP; -.
DR   PhylomeDB; P40396; -.
DR   BioCyc; BSUB:BSU10420-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR   InterPro; IPR010461; ComK.
DR   Pfam; PF06338; ComK; 1.
DR   PIRSF; PIRSF011560; ComK; 1.
PE   1: Evidence at protein level;
KW   Activator; Competence; DNA-binding; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..192
FT                   /note="Competence transcription factor"
FT                   /id="PRO_0000090012"
SQ   SEQUENCE   192 AA;  22430 MW;  E028FC1F57D5736D CRC64;
     MSQKTDAPLE SYEVNGATIA VLPEEIDGKI CSKIIEKDCV FYVNMKPLQI VDRSCRFFGS
     SYAGRKAGTY EVTKISHKPP IMVDPSNQIF LFPTLSSTRP QCGWISHVHV KEFKATEFDD
     TEVTFSNGKT MELPISYNSF ENQVYRTAWL RTKFQDRIDH RVPKRQEFML YPKEERTKMI
     YDFILRELGE RY