COMT1_SACOF
ID COMT1_SACOF Reviewed; 362 AA.
AC O82054;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Caffeic acid 3-O-methyltransferase;
DE Short=CAOMT;
DE Short=COMT;
DE EC=2.1.1.68;
DE AltName: Full=S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferase;
GN Name=COMT;
OS Saccharum officinarum (Sugarcane).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Saccharinae; Saccharum;
OC Saccharum officinarum complex.
OX NCBI_TaxID=4547;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Jaronu 60-5; TISSUE=Root;
RA Selman-Housein G., Lopez M., Hernandez D., Civardi L., Miranda F.,
RA Rigau J., Puigdomenech P.;
RT "Molecular cloning of cDNAs coding for three sugarcane enzymes involved in
RT lignification.";
RL Plant Sci. 143:163-171(1999).
CC -!- FUNCTION: Catalyzes the conversion of caffeic acid to ferulic acid and
CC of 5-hydroxyferulic acid to sinapic acid. The resulting products may
CC subsequently be converted to the corresponding alcohols that are
CC incorporated into lignins.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-caffeate + S-adenosyl-L-methionine = (E)-ferulate + H(+) +
CC S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:20225, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29749, ChEBI:CHEBI:57770, ChEBI:CHEBI:57856,
CC ChEBI:CHEBI:59789; EC=2.1.1.68;
CC -!- PATHWAY: Aromatic compound metabolism; phenylpropanoid biosynthesis.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Cation-independent O-methyltransferase family. COMT
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU01020}.
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DR EMBL; AJ231133; CAA13175.1; -; mRNA.
DR AlphaFoldDB; O82054; -.
DR SMR; O82054; -.
DR BRENDA; 2.1.1.68; 5522.
DR UniPathway; UPA00711; -.
DR GO; GO:0047763; F:caffeate O-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0009809; P:lignin biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR016461; COMT-like.
DR InterPro; IPR022657; De-COase2_CS.
DR InterPro; IPR001077; O_MeTrfase_dom.
DR InterPro; IPR012967; Plant_MeTrfase_dimerisation.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11746; PTHR11746; 1.
DR Pfam; PF08100; Dimerisation; 1.
DR Pfam; PF00891; Methyltransf_2; 1.
DR PIRSF; PIRSF005739; O-mtase; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS00879; ODR_DC_2_2; 1.
DR PROSITE; PS51683; SAM_OMT_II; 1.
PE 2: Evidence at transcript level;
KW Lignin biosynthesis; Methyltransferase; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..362
FT /note="Caffeic acid 3-O-methyltransferase"
FT /id="PRO_0000063213"
FT REGION 161..179
FT /note="Substrate binding"
FT /evidence="ECO:0000250"
FT ACT_SITE 268
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01020"
FT BINDING 129..135
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 207
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01020"
FT BINDING 230
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01020"
FT BINDING 250
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01020"
FT BINDING 251
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01020"
FT BINDING 264
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01020"
SQ SEQUENCE 362 AA; 39578 MW; 1A5C6755444E2F49 CRC64;
MGSTAEDVAA VADEEACMYA MQLASASILP MTLKNALELG LLEVLQAEAP AGKALAPEEV
VARLPVAPTN PDAADMVDRM LRLLASYDVV KCQMEDKDGK YERRYSAAPV GKWLTPNEDG
VSMAALTLMN QDKVLMESWY YLKDAVLDGG IPFNKAYGMT AFEYHGTDPR FNRVFNEGMK
NHSVIITKKL LEFYTGFEGV STLVDVGGGI GATLHAITSH HPQIKGINFD LPHVISEAPP
FPGVQHVGGD MFKSVPAGDA ILMKWILHDW SDAHCATLLK NCYDALPENG KVIIVECVLP
VNTEAVPKAQ GVFHVDMIML AHNPGGRERY EREFHDLAKG AGFSGFKATY IYANAWAIEF
IK
