ID   COMX_BACIU              Reviewed;          58 AA.
AC   P0CY50; Q8VL79;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=ComX pheromone {ECO:0000305};
DE   AltName: Full=Competence pheromone {ECO:0000305};
DE   Flags: Precursor;
GN   Name=comX {ECO:0000303|PubMed:11133937};
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / RO-A-4, and 168 / RO-PP-2;
RX   PubMed=12067344; DOI=10.1046/j.1365-2958.2002.02977.x;
RA   Ansaldi M., Marolt D., Stebe T., Mandic-Mulec I., Dubnau D.;
RT   "Specific activation of the Bacillus quorum-sensing systems by
RT   isoprenylated pheromone variants.";
RL   Mol. Microbiol. 44:1561-1573(2002).
RN   [2]
RP   POLYMORPHISM IN COMX; COMQ AND COMP.
RX   PubMed=11133937; DOI=10.1128/jb.183.2.451-460.2001;
RA   Tortosa P., Logsdon L., Kraigher B., Itoh Y., Mandic-Mulec I., Dubnau D.;
RT   "Specificity and genetic polymorphism of the Bacillus competence quorum-
RT   sensing system.";
RL   J. Bacteriol. 183:451-460(2001).
RN   [3]
RP   POLYMORPHISM, AND DETERMINANTS OF MODIFICATION SPECIFICITY.
RX   PubMed=14679219; DOI=10.1128/jb.186.1.15-21.2004;
RA   Ansaldi M., Dubnau D.;
RT   "Diversifying selection at the Bacillus quorum-sensing locus and
RT   determinants of modification specificity during synthesis of the ComX
RT   pheromone.";
RL   J. Bacteriol. 186:15-21(2004).
CC   -!- FUNCTION: Part of a major quorum-sensing system that regulates the
CC       development of genetic competence (By similarity). Acts through the
CC       activation of the two-component regulatory system ComP/ComA composed of
CC       a sensor histidine kinase, ComP, and a response regulator, ComA (By
CC       similarity). {ECO:0000250|UniProtKB:P45453}.
CC   -!- SUBUNIT: Interacts directly with the sensor histidine kinase ComP and
CC       stimulates its activity. {ECO:0000250|UniProtKB:P45453}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: Trp-56 is modified by isoprenylation, which is essential for
CC       activity (By similarity). Modified by the tryptophan prenyltransferase
CC       ComQ before export to the extracellular environment (By similarity).
CC       The type of isoprenyl derivative differs among the different pherotypes
CC       and depends on ComX primary sequence (PubMed:12067344,
CC       PubMed:14679219). {ECO:0000250|UniProtKB:P45453,
CC       ECO:0000269|PubMed:12067344, ECO:0000269|PubMed:14679219}.
CC   -!- MISCELLANEOUS: The DNA sequences encoding ComQ, ComX and the N-terminal
CC       two-thirds of ComP show a striking polymorphism, which determines the
CC       specificity of the quorum-sensing system in the different pherotypes of
CC       Bacillus. In ComX, the sole conserved residue is the modified
CC       tryptophan essential for the activity. {ECO:0000269|PubMed:11133937}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF456131; AAL67719.1; -; Genomic_DNA.
DR   EMBL; AF456132; AAL67722.1; -; Genomic_DNA.
DR   RefSeq; WP_041906074.1; NZ_SIWZ01000001.1.
DR   AlphaFoldDB; P0CY50; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005186; F:pheromone activity; IEA:UniProtKB-KW.
DR   GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR009233; Competence_ComX_Bacillus.
DR   Pfam; PF05952; ComX; 1.
PE   3: Inferred from homology;
KW   Competence; Lipoprotein; Pheromone; Prenylation; Secreted; Sporulation.
FT   PROPEP          1..52
FT                   /id="PRO_0000233036"
FT   PEPTIDE         53..58
FT                   /note="ComX pheromone"
FT                   /id="PRO_0000233037"
FT   MOD_RES         56
FT                   /note="Tryptophan derivative"
FT                   /evidence="ECO:0000250|UniProtKB:P45453"
SQ   SEQUENCE   58 AA;  6684 MW;  BC6095F268EC1EFE CRC64;
     MKQDMIDYLM KNPQVLTKLE NGEASLIGIP DKLIPSIVDI FNKKMTLSKK CKGIFWEQ