COMX_BACSC
ID COMX_BACSC Reviewed; 58 AA.
AC P0CY51; Q8VL79;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 17.
DE RecName: Full=ComX pheromone {ECO:0000303|PubMed:14679219};
DE AltName: Full=Competence pheromone {ECO:0000305};
DE Flags: Precursor;
GN Name=comX {ECO:0000303|PubMed:11133937};
OS Bacillus spizizenii (Bacillus subtilis subsp. spizizenii).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=96241;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ISOPRENYLATION AT TRP-56, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=DV3-D-2 / NRRL B-23063, and RO-E-2 / NRRL B-23055;
RX PubMed=12067344; DOI=10.1046/j.1365-2958.2002.02977.x;
RA Ansaldi M., Marolt D., Stebe T., Mandic-Mulec I., Dubnau D.;
RT "Specific activation of the Bacillus quorum-sensing systems by
RT isoprenylated pheromone variants.";
RL Mol. Microbiol. 44:1561-1573(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DV3-D-2 / NRRL B-23063;
RA Sabotic J., Cepon U., Cadez P., Mandic Mulec I.;
RT "Variability of Bacillus sp. quorum sensing system.";
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP POLYMORPHISM IN COMX; COMQ AND COMP.
RX PubMed=11133937; DOI=10.1128/jb.183.2.451-460.2001;
RA Tortosa P., Logsdon L., Kraigher B., Itoh Y., Mandic-Mulec I., Dubnau D.;
RT "Specificity and genetic polymorphism of the Bacillus competence quorum-
RT sensing system.";
RL J. Bacteriol. 183:451-460(2001).
RN [4]
RP POLYMORPHISM, AND DETERMINANTS OF MODIFICATION SPECIFICITY.
RX PubMed=14679219; DOI=10.1128/jb.186.1.15-21.2004;
RA Ansaldi M., Dubnau D.;
RT "Diversifying selection at the Bacillus quorum-sensing locus and
RT determinants of modification specificity during synthesis of the ComX
RT pheromone.";
RL J. Bacteriol. 186:15-21(2004).
RN [5]
RP GERANYLATION AT TRP-56.
RC STRAIN=RO-E-2 / NRRL B-23055;
RX PubMed=16407988; DOI=10.1038/nchembio709;
RA Okada M., Sato I., Cho S.J., Iwata H., Nishio T., Dubnau D., Sakagami Y.;
RT "Structure of the Bacillus subtilis quorum-sensing peptide pheromone
RT ComX.";
RL Nat. Chem. Biol. 1:23-24(2005).
RN [6]
RP GERANYLATION AT TRP-56 BY COMQ.
RC STRAIN=RO-E-2 / NRRL B-23055;
RX PubMed=22197102; DOI=10.1016/j.febslet.2011.12.012;
RA Tsuji F., Ishihara A., Kurata K., Nakagawa A., Okada M., Kitamura S.,
RA Kanamaru K., Masuda Y., Murakami K., Irie K., Sakagami Y.;
RT "Geranyl modification on the tryptophan residue of ComXRO-E-2 pheromone by
RT a cell-free system.";
RL FEBS Lett. 586:174-179(2012).
CC -!- FUNCTION: Part of a major quorum-sensing system that regulates the
CC development of genetic competence (PubMed:12067344). Acts through the
CC activation of the two-component regulatory system ComP/ComA composed of
CC a sensor histidine kinase, ComP, and a response regulator, ComA (By
CC similarity). {ECO:0000250|UniProtKB:P45453,
CC ECO:0000269|PubMed:12067344}.
CC -!- SUBUNIT: Interacts directly with the sensor histidine kinase ComP and
CC stimulates its activity. {ECO:0000250|UniProtKB:P45453}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P45453}.
CC -!- PTM: Trp-56 is modified by geranylation, which is essential for
CC activity (PubMed:12067344, PubMed:16407988, PubMed:22197102). Modified
CC by the tryptophan prenyltransferase ComQ before export to the
CC extracellular environment (PubMed:22197102). The type of isoprenyl
CC derivative differs among the different pherotypes and depends on ComX
CC primary sequence (PubMed:12067344, PubMed:14679219).
CC {ECO:0000269|PubMed:12067344, ECO:0000269|PubMed:14679219,
CC ECO:0000269|PubMed:16407988, ECO:0000269|PubMed:22197102}.
CC -!- MISCELLANEOUS: The DNA sequences encoding ComQ, ComX and the N-terminal
CC two-thirds of ComP show a striking polymorphism, which determines the
CC specificity of the quorum-sensing system in the different pherotypes of
CC Bacillus. In ComX, the sole conserved residue is the modified
CC tryptophan essential for the activity. {ECO:0000269|PubMed:11133937}.
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DR EMBL; AF456138; AAL67740.1; -; Genomic_DNA.
DR EMBL; AF458695; AAL59650.1; -; Genomic_DNA.
DR EMBL; DQ241785; ABB51994.1; -; Genomic_DNA.
DR RefSeq; WP_041906074.1; NZ_JACJGE010000005.1.
DR AlphaFoldDB; P0CY51; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005186; F:pheromone activity; IEA:UniProtKB-KW.
DR GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR InterPro; IPR009233; Competence_ComX_Bacillus.
DR Pfam; PF05952; ComX; 1.
PE 1: Evidence at protein level;
KW Competence; Lipoprotein; Pheromone; Prenylation; Secreted.
FT PROPEP 1..52
FT /id="PRO_0000409887"
FT PEPTIDE 53..58
FT /note="ComX pheromone"
FT /id="PRO_0000409888"
FT LIPID 56
FT /note="3'-geranyl-2',N2-cyclotryptophan; in strain RO-E-2 /
FT NRRL B-23055"
FT /evidence="ECO:0000269|PubMed:16407988,
FT ECO:0000269|PubMed:22197102"
SQ SEQUENCE 58 AA; 6684 MW; BC6095F268EC1EFE CRC64;
MKQDMIDYLM KNPQVLTKLE NGEASLIGIP DKLIPSIVDI FNKKMTLSKK CKGIFWEQ
