COM_BPMU
ID COM_BPMU Reviewed; 62 AA.
AC Q38621;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Translational activator com;
DE AltName: Full=Gene product 54;
DE Short=gp54;
DE AltName: Full=Gene product com;
DE Short=gpCom;
GN Name=com; OrderedLocusNames=Mup54;
OS Escherichia phage Mu (Bacteriophage Mu).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Myoviridae; Muvirus.
OX NCBI_TaxID=10677;
OH NCBI_TaxID=543; Enterobacteriaceae.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6345072; DOI=10.1101/sqb.1983.047.01.075;
RA Kahmann R.;
RT "Methylation regulates the expression of a DNA-modification function
RT encoded by bacteriophage Mu.";
RL Cold Spring Harb. Symp. Quant. Biol. 47:639-646(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11922669; DOI=10.1006/jmbi.2002.5437;
RA Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.;
RT "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like
RT prophages in Haemophilus, Neisseria and Deinococcus.";
RL J. Mol. Biol. 317:337-359(2002).
RN [3]
RP FUNCTION.
RX PubMed=2525424; DOI=10.1016/0092-8674(89)90057-3;
RA Wulczyn F.G., Bolker M., Kahmann R.;
RT "Translation of the bacteriophage Mu mom gene is positively regulated by
RT the phage com gene product.";
RL Cell 57:1201-1210(1989).
RN [4]
RP FUNCTION.
RX PubMed=1826635; DOI=10.1016/0092-8674(91)90160-z;
RA Wulczyn F.G., Kahmann R.;
RT "Translational stimulation: RNA sequence and structure requirements for
RT binding of Com protein.";
RL Cell 65:259-269(1991).
RN [5]
RP FUNCTION, AND COFACTOR.
RX PubMed=1835088; DOI=10.1073/pnas.88.22.10027;
RA Hattman S., Newman L., Murthy H.M., Nagaraja V.;
RT "Com, the phage Mu mom translational activator, is a zinc-binding protein
RT that binds specifically to its cognate mRNA.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:10027-10031(1991).
RN [6]
RP INDUCTION.
RX PubMed=8293968; DOI=10.1093/genetics/135.3.619;
RA Chiang L.W., Howe M.M.;
RT "Mutational analysis of a C-dependent late promoter of bacteriophage Mu.";
RL Genetics 135:619-629(1993).
RN [7]
RP COFACTOR, AND MUTAGENESIS OF CYS-6; CYS-9; CYS-26; CYS-29; CYS-39 AND
RP CYS-47.
RX PubMed=7723029; DOI=10.1016/s0022-2836(05)80153-6;
RA Witkowski R.T., Hattman S., Newman L., Clark K., Tierney D.L.,
RA Penner-Hahn J., McLendon G.;
RT "The zinc coordination site of the bacteriophage Mu translational activator
RT protein, Com.";
RL J. Mol. Biol. 247:753-764(1995).
RN [8]
RP REVIEW.
RX PubMed=10665835; DOI=10.1016/s0163-7258(99)00042-x;
RA Hattman S.;
RT "Unusual transcriptional and translational regulation of the bacteriophage
RT Mu mom operon.";
RL Pharmacol. Ther. 84:367-388(1999).
CC -!- FUNCTION: Trans-acting positive regulator required for mom mRNA
CC translation. Binds the com-mom mRNA 5' and destabilizes a translation
CC inhibition stem to expose mom translation start signals.
CC {ECO:0000269|PubMed:1826635, ECO:0000269|PubMed:1835088,
CC ECO:0000269|PubMed:2525424}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000269|PubMed:1835088, ECO:0000269|PubMed:7723029};
CC Note=One Zn(2+) per molecule. {ECO:0000269|PubMed:1835088,
CC ECO:0000269|PubMed:7723029};
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC Expression of late genes is activated by the viral late transcription
CC activator C. {ECO:0000269|PubMed:8293968}.
CC -!- SIMILARITY: Belongs to the com family. {ECO:0000305}.
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DR EMBL; V01463; CAA24709.1; -; Genomic_DNA.
DR EMBL; AF083977; AAF01130.1; -; Genomic_DNA.
DR RefSeq; NP_050656.1; NC_000929.1.
DR GeneID; 2636254; -.
DR KEGG; vg:2636254; -.
DR Proteomes; UP000002611; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR019294; Translation_reg_Com.
DR Pfam; PF10122; Mu-like_Com; 1.
PE 1: Evidence at protein level;
KW Activator; Host cytoplasm; Late protein; Metal-binding; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..62
FT /note="Translational activator com"
FT /id="PRO_0000077659"
FT ZN_FING 6..29
FT /note="Atypical"
FT /evidence="ECO:0000305"
FT BINDING 6
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000305"
FT BINDING 9
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000305"
FT BINDING 26
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000305"
FT BINDING 29
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000305"
FT MUTAGEN 6
FT /note="C->S: Loss of transactivation ability."
FT /evidence="ECO:0000269|PubMed:7723029"
FT MUTAGEN 9
FT /note="C->S: Loss of transactivation ability."
FT /evidence="ECO:0000269|PubMed:7723029"
FT MUTAGEN 26
FT /note="C->S: Loss of transactivation ability."
FT /evidence="ECO:0000269|PubMed:7723029"
FT MUTAGEN 29
FT /note="C->S: Loss of transactivation ability."
FT /evidence="ECO:0000269|PubMed:7723029"
FT MUTAGEN 39
FT /note="C->S: No effect on transactivation ability."
FT /evidence="ECO:0000269|PubMed:7723029"
FT MUTAGEN 47
FT /note="C->S: No effect on transactivation ability."
FT /evidence="ECO:0000269|PubMed:7723029"
SQ SEQUENCE 62 AA; 7414 MW; 08016BBFBFD3D2B6 CRC64;
MKSIRCKNCN KLLFKADSFD HIEIRCPRCK RHIIMLNACE HPTEKHCGKR EKITHSDETV
RY
