CONA2_LUPAN
ID CONA2_LUPAN Reviewed; 643 AA.
AC F5B8V7;
DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 38.
DE RecName: Full=Conglutin alpha 2 {ECO:0000303|PubMed:21457583};
DE AltName: Allergen=Lup an alpha-conglutin {ECO:0000305};
DE Contains:
DE RecName: Full=Conglutin alpha 2A subunit {ECO:0000250|UniProtKB:P04347};
DE Contains:
DE RecName: Full=Conglutin alpha 2B subunit {ECO:0000250|UniProtKB:P04347};
DE Flags: Precursor;
GN ORFNames=TanjilG_32393 {ECO:0000312|EMBL:OIV90516.1};
OS Lupinus angustifolius (Narrow-leaved blue lupine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC genistoids sensu lato; core genistoids; Genisteae; Lupinus.
OX NCBI_TaxID=3871;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND ALLERGEN.
RC STRAIN=cv. Tanjil; TISSUE=Seed;
RX PubMed=21457583; DOI=10.1186/1471-2229-11-59;
RA Foley R.C., Gao L.-L., Spriggs A., Soo L.Y.C., Goggin D.E., Smith P.M.C.,
RA Atkins C.A., Singh K.B.;
RT "Identification and characterisation of seed storage protein transcripts
RT from Lupinus angustifolius.";
RL BMC Plant Biol. 11:59-59(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Tanjil; TISSUE=Seedling;
RX PubMed=27557478; DOI=10.1111/pbi.12615;
RA Hane J.K., Ming Y., Kamphuis L.G., Nelson M.N., Garg G., Atkins C.A.,
RA Bayer P.E., Bravo A., Bringans S., Cannon S., Edwards D., Foley R.,
RA Gao L.L., Harrison M.J., Huang W., Hurgobin B., Li S., Liu C.W.,
RA McGrath A., Morahan G., Murray J., Weller J., Jian J., Singh K.B.;
RT "A comprehensive draft genome sequence for lupin (Lupinus angustifolius),
RT an emerging health food: insights into plant-microbe interactions and
RT legume evolution.";
RL Plant Biotechnol. J. 15:318-330(2017).
RN [3]
RP SUBUNIT.
RC STRAIN=cv. Zeus;
RX PubMed=22264085; DOI=10.1021/jf2042592;
RA Czubinski J., Dwiecki K., Siger A., Kachlicki P., Neunert G.,
RA Lampart-Szczapa E., Nogala-Kalucka M.;
RT "Release of flavonoids from lupin globulin proteins during digestion in a
RT model system.";
RL J. Agric. Food Chem. 60:1830-1836(2012).
CC -!- FUNCTION: Sulfur-rich seed storage protein. This protein found in the
CC seeds of many leguminous and non-leguminous plants is the source of
CC sulfur-containing amino acids in seed meals.
CC {ECO:0000269|PubMed:21457583}.
CC -!- SUBUNIT: Hexamer; each subunit is composed of an acidic and a basic
CC chain derived from a single precursor and linked by a disulfide bond
CC (By similarity). Component of globulins complexes which accumulate in
CC seeds (Probable). {ECO:0000250|UniProtKB:P04347,
CC ECO:0000305|PubMed:22264085}.
CC -!- DEVELOPMENTAL STAGE: Accumulates during seed development.
CC {ECO:0000269|PubMed:21457583}.
CC -!- ALLERGEN: Causes an allergic reaction in human.
CC {ECO:0000305|PubMed:21457583}.
CC -!- SIMILARITY: Belongs to the 11S seed storage protein (globulins) family.
CC {ECO:0000305}.
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DR EMBL; HQ670407; AEB33710.1; -; mRNA.
DR EMBL; KV862168; OIV90516.1; -; Genomic_DNA.
DR EMBL; CM007380; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_019429051.1; XM_019573506.1.
DR AlphaFoldDB; F5B8V7; -.
DR SMR; F5B8V7; -.
DR STRING; 3871.F5B8V7; -.
DR EnsemblPlants; OIV90516; OIV90516; TanjilG_32393.
DR GeneID; 109336727; -.
DR Gramene; OIV90516; OIV90516; TanjilG_32393.
DR KEGG; lang:109336727; -.
DR OMA; PDNRVES; -.
DR OrthoDB; 603461at2759; -.
DR Proteomes; UP000188354; Chromosome LG20.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.10; -; 3.
DR InterPro; IPR022379; 11S_seedstore_CS.
DR InterPro; IPR006044; 11S_seedstore_pln.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 2.
DR PRINTS; PR00439; 11SGLOBULIN.
DR SMART; SM00835; Cupin_1; 2.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS00305; 11S_SEED_STORAGE; 1.
PE 1: Evidence at protein level;
KW Allergen; Disulfide bond; Reference proteome; Seed storage protein; Signal;
KW Storage protein.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..643
FT /note="Conglutin alpha 2"
FT /id="PRO_5010895118"
FT CHAIN 23..457
FT /note="Conglutin alpha 2A subunit"
FT /evidence="ECO:0000250|UniProtKB:P04347"
FT /id="PRO_0000446135"
FT CHAIN 458..630
FT /note="Conglutin alpha 2B subunit"
FT /evidence="ECO:0000250|UniProtKB:P04347"
FT /id="PRO_0000446136"
FT DOMAIN 36..261
FT /note="Cupin type-1 1"
FT /evidence="ECO:0000255"
FT DOMAIN 470..616
FT /note="Cupin type-1 2"
FT /evidence="ECO:0000255"
FT REGION 110..142
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..243
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 285..458
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 623..643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..126
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 220..235
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 299..313
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 325..348
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 356..379
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 430..458
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 31..64
FT /evidence="ECO:0000250|UniProtKB:P04347"
FT DISULFID 107..464
FT /note="Interchain (between A and B chains)"
FT /evidence="ECO:0000250|UniProtKB:P04347"
SQ SEQUENCE 643 AA; 74194 MW; 8A18C45A9A727165 CRC64;
MAKPCLFSFS LCLLLLSSLC LAERPERYKE CQLDRLNALE PDNRVESEGG VTETWNSNRP
ELRCAGVAFE KHTIQPQGLH LPSYTNYPQL IFIVEGEGAL GISVPGCTET YEEAQQSQSS
QDPRRRSSRS QSQEQEQQDS HQKIQYFREG DIIAIPPGIP YWTYNYGEQR LVAINLLDTT
SLLNQLDPSP RRFYIAGNPE EEHPETQEQQ GQQREQQQGA GGRRRGKHQQ EQEEEGKNNV
LSGFDPQFLT QAFNVDEEII NRLQNPDERL KQIVRVKRGL SIISPKSQEE EEEEEEEPRQ
RGQPERREER REEEKEEEEE EDEPRSRERY ERQSRRRPGR QQGRQGEEQE EESESEQEGR
GQQREWERTT RHRRAQGEEG EEEEEETSTR VRRQQGRGRG QEQGQEQGQE QEQEEEQQEG
RRGRHGGRGR RSGQQREEEE EEQQQQQGRR KRQESRNGLE ETICTARLLE NIAKPSRADL
YNPNAGRISS VNSLTLPILR WFQLSADYVN LYRNGIYAPH WNINANSVIF VTRGRGRVQV
VNCQGNSVFN DDLRRGQLLV VPQNFVVAHQ AGDEGFEFIA FKTNDLAATS PVKQVFRGIP
AEVLANAFGL RLNQVSQLKY SGNQGPLVSP QSESEDHTLP KVA
