CONA3_LUPAN
ID CONA3_LUPAN Reviewed; 585 AA.
AC F5B8V8; C9WC98;
DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 41.
DE RecName: Full=Conglutin alpha 3 {ECO:0000303|PubMed:21457583};
DE AltName: Allergen=Lup an alpha-conglutin {ECO:0000305};
DE Contains:
DE RecName: Full=Conglutin alpha 3A subunit {ECO:0000250|UniProtKB:P04347};
DE Contains:
DE RecName: Full=Conglutin alpha 3B subunit {ECO:0000250|UniProtKB:P04347};
DE Flags: Precursor;
GN ORFNames=TanjilG_01206 {ECO:0000312|EMBL:OIW01699.1};
OS Lupinus angustifolius (Narrow-leaved blue lupine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC genistoids sensu lato; core genistoids; Genisteae; Lupinus.
OX NCBI_TaxID=3871;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND ALLERGEN.
RC STRAIN=cv. Tanjil; TISSUE=Seed;
RX PubMed=21457583; DOI=10.1186/1471-2229-11-59;
RA Foley R.C., Gao L.-L., Spriggs A., Soo L.Y.C., Goggin D.E., Smith P.M.C.,
RA Atkins C.A., Singh K.B.;
RT "Identification and characterisation of seed storage protein transcripts
RT from Lupinus angustifolius.";
RL BMC Plant Biol. 11:59-59(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Tanjil; TISSUE=Seedling;
RX PubMed=27557478; DOI=10.1111/pbi.12615;
RA Hane J.K., Ming Y., Kamphuis L.G., Nelson M.N., Garg G., Atkins C.A.,
RA Bayer P.E., Bravo A., Bringans S., Cannon S., Edwards D., Foley R.,
RA Gao L.L., Harrison M.J., Huang W., Hurgobin B., Li S., Liu C.W.,
RA McGrath A., Morahan G., Murray J., Weller J., Jian J., Singh K.B.;
RT "A comprehensive draft genome sequence for lupin (Lupinus angustifolius),
RT an emerging health food: insights into plant-microbe interactions and
RT legume evolution.";
RL Plant Biotechnol. J. 15:318-330(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 57-585.
RC STRAIN=cv. Merrit;
RA Smith P.M., Goggin D.E., Cameron E.C.;
RT "Cloning of Lupinus angustifolius seed storage protein genes.";
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP SUBUNIT.
RC STRAIN=cv. Zeus;
RX PubMed=22264085; DOI=10.1021/jf2042592;
RA Czubinski J., Dwiecki K., Siger A., Kachlicki P., Neunert G.,
RA Lampart-Szczapa E., Nogala-Kalucka M.;
RT "Release of flavonoids from lupin globulin proteins during digestion in a
RT model system.";
RL J. Agric. Food Chem. 60:1830-1836(2012).
CC -!- FUNCTION: Sulfur-rich seed storage protein. This protein found in the
CC seeds of many leguminous and non-leguminous plants is the source of
CC sulfur-containing amino acids in seed meals.
CC {ECO:0000269|PubMed:21457583}.
CC -!- SUBUNIT: Hexamer; each subunit is composed of an acidic and a basic
CC chain derived from a single precursor and linked by a disulfide bond
CC (By similarity). Component of globulins complexes which accumulate in
CC seeds (Probable). {ECO:0000250|UniProtKB:P04347,
CC ECO:0000305|PubMed:22264085}.
CC -!- DEVELOPMENTAL STAGE: Accumulates during seed development.
CC {ECO:0000269|PubMed:21457583}.
CC -!- ALLERGEN: Causes an allergic reaction in human.
CC {ECO:0000305|PubMed:21457583}.
CC -!- SIMILARITY: Belongs to the 11S seed storage protein (globulins) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACN39600.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; HQ670408; AEB33711.1; -; mRNA.
DR EMBL; KV861864; OIW01699.1; -; Genomic_DNA.
DR EMBL; CM007371; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; FJ263366; ACN39600.1; ALT_INIT; mRNA.
DR RefSeq; XP_019462298.1; XM_019606753.1.
DR RefSeq; XP_019462299.1; XM_019606754.1.
DR RefSeq; XP_019462300.1; XM_019606755.1.
DR AlphaFoldDB; F5B8V8; -.
DR SMR; F5B8V8; -.
DR STRING; 3871.F5B8V8; -.
DR EnsemblPlants; OIW01699; OIW01699; TanjilG_01206.
DR GeneID; 109361312; -.
DR Gramene; OIW01699; OIW01699; TanjilG_01206.
DR KEGG; lang:109361312; -.
DR OMA; PHGKRVH; -.
DR OrthoDB; 603461at2759; -.
DR Proteomes; UP000188354; Chromosome LG11.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.10; -; 2.
DR InterPro; IPR022379; 11S_seedstore_CS.
DR InterPro; IPR006044; 11S_seedstore_pln.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 2.
DR PRINTS; PR00439; 11SGLOBULIN.
DR SMART; SM00835; Cupin_1; 2.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS00305; 11S_SEED_STORAGE; 1.
PE 1: Evidence at protein level;
KW Allergen; Disulfide bond; Reference proteome; Seed storage protein; Signal;
KW Storage protein.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..585
FT /note="Conglutin alpha 3"
FT /id="PRO_5010895108"
FT CHAIN 24..399
FT /note="Conglutin alpha 3A subunit"
FT /evidence="ECO:0000250|UniProtKB:P04347"
FT /id="PRO_0000446137"
FT CHAIN 400..572
FT /note="Conglutin alpha 3B subunit"
FT /evidence="ECO:0000250|UniProtKB:P04347"
FT /id="PRO_0000446138"
FT DOMAIN 37..258
FT /note="Cupin type-1 1"
FT /evidence="ECO:0000255"
FT DOMAIN 412..558
FT /note="Cupin type-1 2"
FT /evidence="ECO:0000255"
FT REGION 113..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 199..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 283..402
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 565..585
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 117..147
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..310
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 318..397
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 32..65
FT /evidence="ECO:0000250|UniProtKB:P04347"
FT DISULFID 108..406
FT /note="Interchain (between A and B chains)"
FT /evidence="ECO:0000250|UniProtKB:P04347"
FT CONFLICT 119
FT /note="Q -> R (in Ref. 3; ACN39600)"
FT /evidence="ECO:0000305"
FT CONFLICT 454
FT /note="Y -> C (in Ref. 3; ACN39600)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 585 AA; 67381 MW; E12486240213BC4C CRC64;
MANPFLLSLS LCLVLLYTSA CLGEGLDRFN ECQLDRLNAL EPDNRIESEG GVTETWNSNK
PELRCAGVAF EKHTIEPKGL HLPSYTNYPQ IIMIVQGEGA LGISVPGCTE TFEEAQQSQS
RQERRRGQRS QSQEQEDSHQ KIRHFREGDI LVIPPGTPYW TYNYGDEQLV AINLLDTTSL
SNQLDPNPRR FYLAGNPEEE YPETQQQRQQ RQQHQRPSGR RHGQHQKEEE QEGKNNILSG
FDPQFLSQAL NIDEDTVHKL QNPNERIKQI IRVEEGLGVI SPKWQEQEEE EEEKEEPRQR
RRRERREERE EEEKEEEDEP RESRRHRGGH EEEEVEEERG RGRGGSEWKR TTRRRHTRGD
EGQEEEETTT TTEERRRRRG GRGSRQEEEE EQSPPRSRNG LEETICTAIL RENIADPTRA
DLYNPTAGRI STANSLTLPI LGWFQLSAEY VNLYRNGIYA PHWNINANSV IYVIRGRGRV
QVVNSQGNSV FNDDLRRGQL LVVPQNFVVA HQAGDEGFEF IAFKTNDQAT TSPLKQVFRG
IPAEVLANAF RLSLNQVSEL KYNGNHNPLV TPQSQSQDHN LVKVA
