ID   CONA_CANRO              Reviewed;         236 AA.
AC   P81364;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Concanavalin-Ma;
DE            Short=Con Ma;
OS   Canavalia rosea (Beach bean) (Canavalia maritima).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Canavalia.
OX   NCBI_TaxID=3825;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=1367779; DOI=10.1016/0031-9422(91)85111-c;
RA   Perez G., Perez C., Sousa-Cavada B., Moreira R., Richardson M.;
RT   "Comparison of the amino acid sequences of the lectins from seeds of
RT   Dioclea lehmanni and Canavalia maritima.";
RL   Phytochemistry 30:2619-2621(1991).
CC   -!- FUNCTION: Glucose/D-mannose specific lectin.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- MISCELLANEOUS: Binds one manganese (or another transition metal) ion
CC       and one calcium ion. The metal ions are essential for the saccharide-
CC       binding and cell-agglutinating activities.
CC   -!- SIMILARITY: Belongs to the leguminous lectin family. {ECO:0000305}.
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DR   PIR; B45587; B45587.
DR   PDB; 2CWM; X-ray; 1.95 A; A/D=1-236.
DR   PDB; 2OW4; X-ray; 1.60 A; A=1-236.
DR   PDB; 2P34; X-ray; 2.10 A; A/B/C/D=1-236.
DR   PDB; 2P37; X-ray; 2.10 A; A/B/C/D=1-236.
DR   PDBsum; 2CWM; -.
DR   PDBsum; 2OW4; -.
DR   PDBsum; 2P34; -.
DR   PDBsum; 2P37; -.
DR   SMR; P81364; -.
DR   UniLectin; P81364; -.
DR   EvolutionaryTrace; P81364; -.
DR   GO; GO:0005537; F:mannose binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR000985; Lectin_LegA_CS.
DR   InterPro; IPR019825; Lectin_legB_Mn/Ca_BS.
DR   InterPro; IPR001220; Legume_lectin_dom.
DR   Pfam; PF00139; Lectin_legB; 2.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   PROSITE; PS00308; LECTIN_LEGUME_ALPHA; 1.
DR   PROSITE; PS00307; LECTIN_LEGUME_BETA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Calcium; Direct protein sequencing; Lectin; Manganese;
KW   Mannose-binding; Metal-binding.
FT   CHAIN           1..236
FT                   /note="Concanavalin-Ma"
FT                   /id="PRO_0000105086"
FT   BINDING         8
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         10
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         10
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         12
FT                   /ligand="a carbohydrate"
FT                   /ligand_id="ChEBI:CHEBI:16646"
FT                   /evidence="ECO:0000250"
FT   BINDING         12
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         14
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         19
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         19
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         24
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         98..99
FT                   /ligand="a carbohydrate"
FT                   /ligand_id="ChEBI:CHEBI:16646"
FT                   /evidence="ECO:0000250"
FT   BINDING         207
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         227
FT                   /ligand="a carbohydrate"
FT                   /ligand_id="ChEBI:CHEBI:16646"
FT                   /evidence="ECO:0000250"
FT   STRAND          4..10
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   HELIX           15..17
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          24..33
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          35..38
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          45..54
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   TURN            55..58
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          59..65
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          71..77
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   HELIX           80..82
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          86..95
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          97..99
FT                   /evidence="ECO:0007829|PDB:2CWM"
FT   STRAND          104..120
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          122..131
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          139..143
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          149..151
FT                   /evidence="ECO:0007829|PDB:2CWM"
FT   STRAND          153..156
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          169..176
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          185..197
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          201..204
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   STRAND          208..215
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   HELIX           226..228
FT                   /evidence="ECO:0007829|PDB:2OW4"
FT   TURN            229..231
FT                   /evidence="ECO:0007829|PDB:2OW4"
SQ   SEQUENCE   236 AA;  25364 MW;  D67791C3B0EB150A CRC64;
     ADTIVAVELD TYPNTDVGDP SYPHXXXXXX SVRXXTAKWN MQNGKVGTAH ISYNSVGKRL
     SAVVSYPNGD SATVSYDVDL DNVLPEWVRV GLSASTGLYK ETNTILSWSF TSKLKSNSTH
     ETNALHFMFN QFTKDQKDLI LQSDATTGTD GNLXXTRVSS NGPSQGSTVG RALFYAPVHI
     WESSATVAGF DATFXXLIKS PDSHPADGIA FFISNIDSSI PSGSTGRLLG LFPDAN