CONB_CANEN
ID CONB_CANEN Reviewed; 324 AA.
AC P49347;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Concanavalin B;
DE Short=Con B;
DE Flags: Precursor;
OS Canavalia ensiformis (Jack bean) (Dolichos ensiformis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Canavalia.
OX NCBI_TaxID=3823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC TISSUE=Cotyledon;
RA Schlesier B., Nong V., Horstmann C., Hennig M.;
RL Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).
RX PubMed=7490746; DOI=10.1006/jmbi.1995.0614;
RA Hennig M., Jansonius J.N., van Scheltinga A.C.T., Dijkstra B.W.,
RA Schlesier B.;
RT "Crystal structure of concanavalin B at 1.65-A resolution. An 'inactivated'
RT chitinase from seeds of Canavalia ensiformis.";
RL J. Mol. Biol. 254:237-246(1995).
CC -!- FUNCTION: May act as a carbohydrate-binding protein.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 18 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X83426; CAA58450.1; -; mRNA.
DR PIR; S57649; S57649.
DR PDB; 1CNV; X-ray; 1.65 A; A=26-324.
DR PDB; 6CAF; X-ray; 1.30 A; A=1-324.
DR PDB; 6GWA; X-ray; 2.10 A; A=26-324.
DR PDBsum; 1CNV; -.
DR PDBsum; 6CAF; -.
DR PDBsum; 6GWA; -.
DR AlphaFoldDB; P49347; -.
DR SMR; P49347; -.
DR CAZy; GH18; Glycoside Hydrolase Family 18.
DR EvolutionaryTrace; P49347; -.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd02877; GH18_hevamine_XipI_class_III; 1.
DR InterPro; IPR045321; Cts1-like.
DR InterPro; IPR001223; Glyco_hydro18_cat.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF00704; Glyco_hydro_18; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS51910; GH18_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Glycoprotein; Signal.
FT SIGNAL 1..25
FT CHAIN 26..324
FT /note="Concanavalin B"
FT /id="PRO_0000011989"
FT DOMAIN 30..311
FT /note="GH18"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01258"
FT CARBOHYD 309
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT HELIX 27..29
FT /evidence="ECO:0007829|PDB:6CAF"
FT STRAND 31..36
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 39..41
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 44..49
FT /evidence="ECO:0007829|PDB:6CAF"
FT STRAND 54..61
FT /evidence="ECO:0007829|PDB:6CAF"
FT TURN 76..78
FT /evidence="ECO:0007829|PDB:6CAF"
FT TURN 81..83
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 87..90
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 91..99
FT /evidence="ECO:0007829|PDB:6CAF"
FT STRAND 103..109
FT /evidence="ECO:0007829|PDB:6CAF"
FT STRAND 111..115
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 120..134
FT /evidence="ECO:0007829|PDB:6CAF"
FT STRAND 135..139
FT /evidence="ECO:0007829|PDB:6CAF"
FT STRAND 149..154
FT /evidence="ECO:0007829|PDB:6CAF"
FT STRAND 161..163
FT /evidence="ECO:0007829|PDB:6GWA"
FT HELIX 164..178
FT /evidence="ECO:0007829|PDB:6CAF"
FT STRAND 183..186
FT /evidence="ECO:0007829|PDB:6CAF"
FT STRAND 189..193
FT /evidence="ECO:0007829|PDB:6CAF"
FT TURN 195..197
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 198..202
FT /evidence="ECO:0007829|PDB:6CAF"
FT STRAND 207..212
FT /evidence="ECO:0007829|PDB:6CAF"
FT TURN 217..219
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 227..239
FT /evidence="ECO:0007829|PDB:6CAF"
FT STRAND 240..242
FT /evidence="ECO:0007829|PDB:1CNV"
FT STRAND 247..254
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 255..257
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 266..272
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 274..276
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 280..283
FT /evidence="ECO:0007829|PDB:6CAF"
FT STRAND 284..290
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 292..298
FT /evidence="ECO:0007829|PDB:6CAF"
FT HELIX 300..308
FT /evidence="ECO:0007829|PDB:6CAF"
FT TURN 309..312
FT /evidence="ECO:0007829|PDB:6CAF"
SQ SEQUENCE 324 AA; 36728 MW; 539CA5D4B68397FE CRC64;
MGCERKALIL MVVIWIMSFW TLSLADISST EIAVYWGQRE DGLLRDTCKT NNYKIVFISF
LDKFGCEIRK PELELEGVCG PSVGNPCSFL ESQIKECQRM GVKVFLALGG PKGTYSACSA
DYAKDLAEYL HTYFLSERRE GPLGKVALDG IHFDIQKPVD ELNWDNLLEE LYQIKDVYQS
TFLLSAAPGC LSPDEYLDNA IQTRHFDYIF VRFYNDRSCQ YSTGNIQRIR NAWLSWTKSV
YPRDKNLFLE LPASQATAPG GGYIPPSALI GQVLPYLPDL QTRYAGIALW NRQADKETGY
STNIIRYLNA TAMPFTSNLL KYPS
