ID   CONO2_CONML             Reviewed;           9 AA.
AC   P0DQM7;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   12-AUG-2020, sequence version 1.
DT   07-OCT-2020, entry version 2.
DE   RecName: Full=Conopressin-M2 {ECO:0000303|PubMed:32155768};
OS   Conus miliaris (Thousand-spot cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Virroconus.
OX   NCBI_TaxID=97181;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SYNTHESIS, DISULFIDE BOND, AMIDATION
RP   AT SER-9, AND STRUCTURE BY NMR.
RX   PubMed=32155768; DOI=10.3390/md18030150;
RA   Giribaldi J., Ragnarsson L., Pujante T., Enjalbal C., Wilson D., Daly N.L.,
RA   Lewis R.J., Dutertre S.;
RT   "Synthesis, pharmacological and structural characterization of novel
RT   conopressins from Conus miliaris.";
RL   Mar. Drugs 18:0-0(2020).
CC   -!- FUNCTION: Shows reduced activity vasopressin-oxytocin related
CC       receptors. Is active on fish receptors, but not on their human
CC       counterpart, supporting an evolved role of this conopressin in the
CC       envenomation process. Shows weak agonist activity on the zebrafish
CC       vasopressin-2 receptor (V2R) (EC(50)=1.72 uM). Has no effect on all
CC       other receptors tested. {ECO:0000269|PubMed:32155768}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:32155768}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:32155768}.
CC   -!- DOMAIN: The cysteine framework is C-C. {ECO:0000305}.
CC   -!- PTM: It is unsure whether Ser-9 is amidated or not, it is why the two
CC       forms have been synthesized. The non-amidated form shows a weaker
CC       agonist activity on the zebrafish vasopressin-2 receptor (V2R)
CC       (EC(50)=3.6 uM). The state of the C-terminal residue does not impact
CC       its 3D-structure. {ECO:0000269|PubMed:32155768}.
CC   -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Disulfide bond; G-protein coupled receptor impairing toxin;
KW   Secreted; Toxin.
FT   PEPTIDE         1..9
FT                   /note="Conopressin-M2"
FT                   /evidence="ECO:0000305|PubMed:32155768"
FT                   /id="PRO_0000450674"
FT   SITE            8
FT                   /note="Non-basic residue surely responsible of very weak
FT                   activity"
FT                   /evidence="ECO:0000305|PubMed:32155768"
FT   MOD_RES         9
FT                   /note="Serine amide"
FT                   /evidence="ECO:0000305|PubMed:32155768"
FT   DISULFID        1..6
FT                   /evidence="ECO:0000305|PubMed:32155768"
SQ   SEQUENCE   9 AA;  955 MW;  C935B76EB4487729 CRC64;
     CFLGNCPDS