ID   CONOT_CONTU             Reviewed;           9 AA.
AC   P0DL76;
DT   22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2017, sequence version 1.
DT   07-OCT-2020, entry version 11.
DE   RecName: Full=Conopressin-T {ECO:0000303|PubMed:18174156};
DE            Short=Con-T {ECO:0000303|PubMed:18174156};
OS   Conus tulipa (Fish-hunting cone snail) (Tulip cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Gastridium.
OX   NCBI_TaxID=6495;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-7,
RP   STRUCTURE BY NMR, DISULFIDE BOND, AMIDATION AT VAL-9, MASS SPECTROMETRY,
RP   AND SYNTHESIS.
RC   TISSUE=Venom;
RX   PubMed=18174156; DOI=10.1074/jbc.m706477200;
RA   Dutertre S., Croker D., Daly N.L., Andersson A., Muttenthaler M.,
RA   Lumsden N.G., Craik D.J., Alewood P.F., Guillon G., Lewis R.J.;
RT   "Conopressin-T from Conus tulipa reveals an antagonist switch in
RT   vasopressin-like peptides.";
RL   J. Biol. Chem. 283:7100-7108(2008).
CC   -!- FUNCTION: Interacts with high affinity with oxytocin receptor (OXTR)
CC       (Ki=108 nM) and vasopressin V1a receptor (V1aR/AVPR1A) (Ki=319 nM).
CC       Acts as a partial agonist on OXTR (Kact=37 nM, 22% of oxytocin maximum
CC       activity at 10 uM), and as a full antagonist on V1aR (Kinact=329 nM).
CC       {ECO:0000269|PubMed:18174156}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18174156}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:18174156}.
CC   -!- DOMAIN: The cysteine framework is C-C. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=1107.54; Method=Electrospray; Note=Monoisotopic
CC       mass.; Evidence={ECO:0000269|PubMed:18174156};
CC   -!- MISCELLANEOUS: Does not show binding with vasopressin V1b and V2
CC       receptors (up to 10 uM). Shows a weak agonist activity on V1bR/AVPR1B
CC       (9% at 10 uM). {ECO:0000269|PubMed:18174156}.
CC   -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Biological Magnetic Resonance Data Bank;
CC       URL="http://www.bmrb.wisc.edu/data_library/summary/index.php?bmrbId=20007";
CC   -!- WEB RESOURCE: Name=Biological Magnetic Resonance Data Bank; Note=L7P
CC       mutant;
CC       URL="http://www.bmrb.wisc.edu/data_library/summary/index.php?bmrbId=20008";
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond;
KW   G-protein coupled receptor impairing toxin; Secreted; Toxin.
FT   PEPTIDE         1..9
FT                   /note="Conopressin-T"
FT                   /evidence="ECO:0000269|PubMed:18174156"
FT                   /id="PRO_0000442232"
FT   SITE            9
FT                   /note="Important residue for antagonist activity on
FT                   V1aR/AVPR1A (a Gly in this position produces agonist
FT                   activity)"
FT                   /evidence="ECO:0000269|PubMed:18174156"
FT   MOD_RES         9
FT                   /note="Valine amide"
FT                   /evidence="ECO:0000269|PubMed:18174156"
FT   DISULFID        1..6
FT                   /evidence="ECO:0000269|PubMed:18174156"
FT   MUTAGEN         7
FT                   /note="L->P: 8-fold increase in affinity for V1aR/AVPR1A,
FT                   no change in affinity for V1b and oxytocin receptors (OXTR)
FT                   and gain of affinity for V2R (Ki=1.8 uM)."
FT                   /evidence="ECO:0000269|PubMed:18174156"
SQ   SEQUENCE   9 AA;  1111 MW;  0D5B072EB456D04B CRC64;
     CYIQNCLRV